Surface charge modulates the internalization vs. penetration of gold nanoparticles: comprehensive scrutiny on monolayer cancer cells, multicellular spheroids and solid tumors by SERS modality

Nanoscale ◽  
2020 ◽  
Vol 12 (13) ◽  
pp. 6971-6975 ◽  
Author(s):  
Palasseri T. Sujai ◽  
Manu M. Joseph ◽  
Giridharan Saranya ◽  
Jyothi B. Nair ◽  
Vishnu Priya Murali ◽  
...  
Keyword(s):  
Cell Culture ◽  
Gold Nanoparticles ◽  
Surface Charge ◽  
Cancer Cells ◽  
Solid Tumors ◽  
Mouse Models ◽  
Surface Charges ◽  
Multicellular Spheroids ◽  
Differential Distribution ◽  
Monolayer Cell Culture

Differential distribution of gold nanoparticles with respect to surface charges on monolayer cell culture, multicellular spheroids and in mouse models.

In-Vitro Study of Cancer Cell Binding and Photothermal Inhibition Efficiency by Gold Nanoparticles-Capped Iron Oxide Nanospheres

Nano LIFE ◽  
2019 ◽  
Vol 09 (01n02) ◽  
pp. 1940004
Author(s):  
Zi Yang ◽  
Haocheng Yang ◽  
Donglu Shi ◽  
Yilong Wang
Keyword(s):  
Gold Nanoparticles ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Surface Charges ◽  
Cell Binding ◽  
Au Nps ◽  
Photothermal Property ◽  
Positively Charged

Different from the traditional cancer cell targeting through protein biomarkers, strong negative surface charges of cancer cells over normal cells derived from the dramatic excretion of lactate acid by cancer cells due to their glycolysis have been testified as an effective cancer cell binding strategy. In our previous work, polyethylenimine (PEI)-functionalized iron oxide nanoparticles (NPs) with strong positive surface charge and good photothermal property have been applied to kill cancer cells in vitro. Considering the obvious cytotoxicity of the first generation of positively charged nanotherapeutics, second generation of magnetic nanotherapeutics with tunable surface charges was designed and prepared by shield of positively charged surface areas by negatively charged gold nanoparticles (AU NPs). In addition to reducing the cytotoxicity of original NPs, the photothermal property of magnetic NP core was simultaneously enhanced. Herein, the correlation of the capping degree of the positive surface charge by AU NPs and the photothermal inhibition of cancer cells was investigated.

scholarly journals Size-Dependent Localization and Penetration of Ultrasmall Gold Nanoparticles in Cancer Cells, Multicellular Spheroids, and Tumors in Vivo

ACS Nano ◽  
2012 ◽  
Vol 6 (5) ◽  
pp. 4483-4493 ◽  
Author(s):  
Keyang Huang ◽  
Huili Ma ◽  
Juan Liu ◽  
Shuaidong Huo ◽  
Anil Kumar ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Multicellular Spheroids ◽  
Size Dependent

scholarly journals Massive programmed cell death in intestinal epithelial cells induced by three-dimensional growth conditions: suppression by mutant c-H-ras oncogene expression.

1995 ◽  
Vol 131 (6) ◽  
pp. 1587-1598 ◽  
Author(s):  
J Rak ◽  
Y Mitsuhashi ◽  
V Erdos ◽  
S N Huang ◽  
J Filmus ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Death ◽  
Epithelial Cells ◽  
Programmed Cell Death ◽  
Intestinal Epithelial Cells ◽  
Three Dimensional ◽  
Intestinal Epithelial ◽  
Ras Oncogene ◽  
Multicellular Spheroids ◽  
Monolayer Cell Culture

Deregulation of molecular pathways controlling cell survival and death, including programmed cell death, are thought to be important factors in tumor formation, disease progression, and response to therapy. Studies devoted to analyzing the role of programmed cell death in cancer have been carried out primarily using conventional monolayer cell culture systems. However the majority of cancers grow as three-dimensional solid tumors. Because gene expression, and possibly function, can be significantly altered under such conditions, we decided to analyze the control and characteristics of cell death using a compatible three-dimensional tissue culture system (multicellular spheroids) and compare the results obtained to those using two-dimensional monolayer cell culture. To do so we selected for study an immortalized, but nontumorigenic line of rat intestinal epithelial cells, called IEC-18, and several tumorigenic variants of IEC-18 obtained by transfection with a mutant (activated) c-H-ras oncogene. The rationale for choosing these cell lines was based in part on the fact that intestinal epithelial cells grow in vivo in a monolayer-like manner and form solid tumors only after sustaining certain genetic mutations, including those involving the ras gene family. We found that the IEC-18 cells, which grow readily and survive in monolayer cell culture, undergo massive cell death within 48-72 h when cultured as multicellular spheroids on a nonadhesive surface. This process was accompanied by a number of features associated with programmed cell death including chromatin condensation (Hoechst 33258 staining) apoptotic morphology, DNA degradation, and a virtual complete loss of colony forming (clonogenic) ability in the absence of apparent membrane damage as well as accumulation of lipid containing vacuoles in the cytoplasm. Moreover, enforced over-expression of a transfected bcl-2 gene could prevent this cell death process from taking place. In marked contrast, three different stably transfected ras clones of IEC-18 survived when grown as multicellular spheroids. In addition, an IEC cell line (called clone 25) carrying its mutant transfected ras under a glucocorticoid inducible promoter survived in three-dimensional culture only when the cells were exposed to dexamethasone. If exposure to dexamethasone was delayed for as long as 48 h the cells nevertheless survived, whereas the cells became irreversibly committed to programmed cell death (PCD) if exposed to dexamethasone after 72 h. These results suggest that intestinal epithelial cells may be programmed to activate a PCD pathway upon detachment from a physiologic two-dimensional monolayer configuration, and that this process of adhesion regulated programmed cell death (ARPCD) can be substantially suppressed by expression of a mutant ras oncogene.(ABSTRACT TRUNCATED AT 400 WORDS)

scholarly journals Effect of Modification Protocols on the Effectiveness of Gold Nanoparticles as Drug Delivery Vehicles for Killing of Breast Cancer Cells

2016 ◽  
Vol 69 (12) ◽  
pp. 1402 ◽  
Author(s):  
Zahrah Alhalili ◽  
Daniela Figueroa ◽  
Martin R. Johnston ◽  
Joe Shapter ◽  
Barbara Sanderson
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Culture Media ◽  
High Dose ◽  
Dependent Manner ◽  
T47d Cells

The current study evaluated the potential of gold nanoparticles (AuNPs) for the delivery of Taxol to breast cancer cells (T47D) using an in vitro cell culture model. For this study, new loading approaches and novel chemical attachments were investigated. Five different gold nanoparticle-based complexes were used to determine their cytotoxicity towards T47D cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assay. There was no significant decrease (P > 0.05) in cell viability when T47D cells were treated with AuNPs that did not contain Taxol. However, cells were significantly killed by gold nanoparticles chemically conjugated to Taxol using three different approaches and one novel hybrid AuNP-Taxol nanoparticle, wherein no chemical bonds were involved. These Taxol-loaded AuNPs were more effective at inducing cell death in vitro than a solution of free Taxol used to treat cells. This result demonstrated that Taxol could be released from the particles in the cell culture media for subsequent therapeutic action. Additionally, the experiments proved that the Taxol-loaded AuNPs were more toxic in a dose dependent manner than Taxol as a formulation for the treatment of breast cancer cells. The results of this study suggest that gold nanoparticles have potential for the efficient delivery of Taxol to breast cancer cells. This could provide a future solution as an alternative application method to overcome adverse side effects resulting from current high-dose treatment regimes.

scholarly journals One-Step Synthesis of PEGylated Gold Nanoparticles with Tunable Surface Charge

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Rares Stiufiuc ◽  
Cristian Iacovita ◽  
Raul Nicoara ◽  
Gabriela Stiufiuc ◽  
Adrian Florea ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Charge ◽  
Colloidal Stability ◽  
Spherical Shape ◽  
Average Diameter ◽  
Molecular Chain ◽  
Surface Charges ◽  
One Step

The present work reports a rapid, simple and efficient one-step synthesis and detailed characterisation of stable aqueous colloids of gold nanoparticles (AuNPs) coated with unmodified poly(ethylene)glycol (PEG) molecules of different molecular weights and surface charges. By mixing and heating aqueous solutions of PEG with variable molecular chain and gold(III) chloride hydrate (HAuCl4) in the presence of NaOH, we have successfully produced uniform colloidal 5 nm PEG coated AuNPs of spherical shape with tunable surface charge and an average diameter of 30 nm within a few minutes. It has been found out that PEGylated AuNPs provide optical enhancement of the characteristic vibrational bands of PEG molecules attached to the gold surface when they are excited with both visible (532 nm) and NIR (785 nm) laser lines. The surface enhanced Raman scattering (SERS) signal does not depend on the length of the PEG molecular chain enveloping the AuNPs, and the stability of the colloid is not affected by the addition of concentrated salt solution (0.1 M NaCl), thus suggesting their potential use forin vitroandin vivoapplications. Moreover, by gradually changing the chain length of the biopolymer, we were able to control nanoparticles’ surface charge from −28 to −2 mV, without any modification of the Raman enhancement properties and of the colloidal stability.

Gold nanoparticles decorated with oligo(ethylene glycol) thiols: Surface charges and interactions with proteins in solution

2014 ◽  
Vol 426 ◽  
pp. 31-38 ◽  
Author(s):  
Moritz Schollbach ◽  
Fajun Zhang ◽  
Felix Roosen-Runge ◽  
Maximilian W.A. Skoda ◽  
Robert M.J. Jacobs ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Ethylene Glycol ◽  
Surface Charges
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