scholarly journals Size-Dependent Localization and Penetration of Ultrasmall Gold Nanoparticles in Cancer Cells, Multicellular Spheroids, and Tumors in Vivo

ACS Nano ◽  
2012 ◽  
Vol 6 (5) ◽  
pp. 4483-4493 ◽  
Author(s):  
Keyang Huang ◽  
Huili Ma ◽  
Juan Liu ◽  
Shuaidong Huo ◽  
Anil Kumar ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Multicellular Spheroids ◽  
Size Dependent

Receptor-Mediated In Vivo Targeting of Breast Cancer Cells with 17α-Ethynylestradiol-Conjugated Silica-Coated Gold Nanoparticles

Langmuir ◽  
2020 ◽  
Vol 36 (48) ◽  
pp. 14819-14828
Author(s):  
Alexander M. Renner ◽  
Shaista Ilyas ◽  
Hans A. Schlößer ◽  
Annika Szymura ◽  
Stefan Roitsch ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells

scholarly journals Targeting Cancer Resistance via Multifunctional Gold Nanoparticles

2019 ◽  
Vol 20 (21) ◽  
pp. 5510 ◽  
Author(s):  
Pedro Pedrosa ◽  
M. Luísa Corvo ◽  
Margarida Ferreira-Silva ◽  
Pedro Martins ◽  
Manuela Colla Carvalheiro ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Targeted Delivery ◽  
Drug Stability ◽  
Chemotherapeutic Agents ◽  
In Vivo Studies ◽  
Cancer Resistance ◽  
Selective Targeting ◽  
New Compounds

Resistance to chemotherapy is a major problem facing current cancer therapy, which is continuously aiming at the development of new compounds that are capable of tackling tumors that developed resistance toward common chemotherapeutic agents, such as doxorubicin (DOX). Alongside the development of new generations of compounds, nanotechnology-based delivery strategies can significantly improve the in vivo drug stability and target specificity for overcoming drug resistance. In this study, multifunctional gold nanoparticles (AuNP) have been used as a nanoplatform for the targeted delivery of an original anticancer agent, a Zn(II) coordination compound [Zn(DION)2]Cl2 (ZnD), toward better efficacy against DOX-resistant colorectal carcinoma cells (HCT116 DR). Selective delivery of the ZnD nanosystem to cancer cells was achieved by active targeting via cetuximab, NanoZnD, which significantly inhibited cell proliferation and triggered the death of resistant tumor cells, thus improving efficacy. In vivo studies in a colorectal DOX-resistant model corroborated the capability of NanoZnD for the selective targeting of cancer cells, leading to a reduction of tumor growth without systemic toxicity. This approach highlights the potential of gold nanoformulations for the targeting of drug-resistant cancer cells.

scholarly journals Size-dependent in vivo toxicity of PEG-coated gold nanoparticles

2011 ◽  
pp. 2071 ◽  
Author(s):  
Xiao-Dong Zhang ◽  
Wu ◽  
Shen ◽  
Liu ◽  
Sun ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
In Vivo Toxicity ◽  
Size Dependent

scholarly journals Enhanced radiation sensitivity in prostate cancer by gold-nanoparticles

2008 ◽  
Vol 31 (3) ◽  
pp. 160 ◽  
Author(s):  
Xiaojing Zhang ◽  
James Z. Xing ◽  
Jie Chen ◽  
Lawrence Ko ◽  
John Amanie ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticles ◽  
Growth Inhibition ◽  
Cancer Cells ◽  
Radiation Sensitivity ◽  
Human Prostate ◽  
In Vivo Studies ◽  
Prostate Cancer Cells ◽  
Nanoparticle Uptake

Purpose: Nanotechnology is an emerging field with significant translational potential in medicine. In this study, we applied gold nanoparticles (GNP) to enhance radiation sensitivity and growth inhibition in radiation-resistant human prostate cancer cells. Methods: Gold nanoparticles (GNPs) were synthesized using HAuCl4 as the gold particle source and NaBH4 as the reductant. Either thio-glucose or sodium citrate was then added to the solution separately to bind the GNPs to form thio-glucose-capped gold nanoparticles (Glu-GNP) and neutral gold nanoparticles (TGS-GNPs). Human prostate carcinoma DU-145 cells were exposed to vehicle, irradiation, 15nM TGS-GNPs, or 15nM Glu-GNPs, or GNPs plus irradiation. The uptake assays of GNP were performed using hemocytometer to count cells and the mass spectrometry was applied to calculate gold mass. The cytotoxicity induced by GNPs, irradiation, or GNPs plus irradiation was measured using a standard colorimetric MTT assay. Results: Exposure to Glu-GNPs resulted in a three times increase of nanoparticle uptake compared to that of TGS-GNPs in each target cell (p < 0.005). Cytoplasmic intracellular uptake of both TGS-GNPs and Glu-GNPs resulted in a growth inhibition by 30.57% and 45.97% respectively, comparing to 15.88% induced by irradiation alone, in prostate cancer cells after exposure to the irradiation. Glu-GNPs showed a greater enhancement, 1.5 to 2 fold increases within 72 hours, on irradiation cytotoxicity compared to TGS-GNPs. Tumour killing, however, did not appear to correlate linearly with nanoparticle uptake concentrations. Conclusion: These results showed that functional glucose-bound gold nanoparticles enhanced radiation sensitivity and toxicity in prostate cancer cells. In vivo studies will be followed to verify our research findings.

Surface charge modulates the internalization vs. penetration of gold nanoparticles: comprehensive scrutiny on monolayer cancer cells, multicellular spheroids and solid tumors by SERS modality

Nanoscale ◽  
2020 ◽  
Vol 12 (13) ◽  
pp. 6971-6975 ◽  
Author(s):  
Palasseri T. Sujai ◽  
Manu M. Joseph ◽  
Giridharan Saranya ◽  
Jyothi B. Nair ◽  
Vishnu Priya Murali ◽  
...  
Keyword(s):  
Cell Culture ◽  
Gold Nanoparticles ◽  
Surface Charge ◽  
Cancer Cells ◽  
Solid Tumors ◽  
Mouse Models ◽  
Surface Charges ◽  
Multicellular Spheroids ◽  
Differential Distribution ◽  
Monolayer Cell Culture

Differential distribution of gold nanoparticles with respect to surface charges on monolayer cell culture, multicellular spheroids and in mouse models.

scholarly journals Biodegradable Nanoparticles Combining Cancer Cell Targeting and Anti-Angiogenic Activity for Synergistic Chemotherapy in Epithelial Cancer

2021 ◽  
Author(s):  
Francesca moret ◽  
Claudia Conte ◽  
Diletta Esposito ◽  
Giovanni Dal Poggetto ◽  
Concetta Avitabile ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Folate Receptor ◽  
Tube Formation ◽  
Multicellular Spheroids ◽  
Angiogenic Activity ◽  
Biodegradable Nanoparticles ◽  
Therapeutic Concepts ◽  
Poly Ethylene

Abstract A biodegradable engineered nanoplatform combining anti-angiogenic activity and targeting of cancer cells to improve the anticancer activity of docetaxel (DTX) is here proposed. Indeed, we have developed biodegradable nanoparticles (NPs) of poly(ethylene glycol)-poly(ε-caprolactone), exposing on the surface both folate motifs (Fol) for recognition in cells overexpressing Folate Receptor- a (FRa) and the anti-angiogenic hexapeptide aFLT1. The presence of Fol on NPs did not impair the anti-angiogenic activity of aFLT1, as assessed by in vitro tube formation assay in HUVEC endothelial cells. In both 2D and 3D KB cell cultures in vitro , the cytotoxicity of DTX loaded in NPs was not significantly affected by Fol/aFLT1 double decoration as compared to free DTX. Remarkably, NPs distributed differently in 3D multicellular spheroids of FRa-positive KB cancer cells depending on the type of ligand displayed on the surface. When tested in vivo in zebrafish embryos xenografted with KB cells, NPs displaying Fol/aFLT1 reduced DTX systemic toxicity and inhibited in a synergistic way the growth of the tumor mass and associated vasculature. Overall, nanotechnology offers excellent ground for combining therapeutic concepts in cancer, paving the way to the development of novel multifunctional nanopharmaceuticals where surface decoration with bioactive elements can significantly improve therapeutic outcomes.

scholarly journals The Ultrastructural Analysis of Human Colorectal Cancer Stem Cell-Derived Spheroids and Their Mouse Xenograft Shows That the Same Cells Types Have Different Ratios

Biology ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 929
Author(s):  
Michela Relucenti ◽  
Federica Francescangeli ◽  
Maria Laura De De Angelis ◽  
Vito D'Andrea ◽  
Selenia Miglietta ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Stem Cell ◽  
Cancer Cells ◽  
Drug Testing ◽  
Flow Cytometric Analysis ◽  
Experimental Models ◽  
Stem Cell Marker ◽  
Multicellular Spheroids

Spheroids from primary colorectal cancer cells and their mice xenografts have emerged as useful preclinical models for cancer research as they replicate tumor features more faithfully as compared to cell lines. While 3D models provide a reliable system for drug discovery and testing, their structural complexity represents a challenge and their structure-function relationships are only partly understood. Here, we present a comparative ultrastructural and flow citometric analysis of patient colorectal cancer-derived spheroids and their mice xenografts. Ultrastructural observations highlighted that multicellular spheroids and their xenografts contain the same cancer cell types but with different ratios, specifically multicellular spheroids were enriched in cells with a stem-like phenotype, while xenografts had an increased amount of lipid droplets-containing cells. The flow cytometric analysis for stem cell marker and activity showed enrichment of stem-like cells presence and activity in spheroids while xenografts had the inverse response. Our results evidence the effects on cancer cells of different in vitro and in vivo microenvironments. Those differences have to be paid into account in designing innovative experimental models for personalized drug testing.

scholarly journals Size-Dependent Interactions of Lipid-Coated Gold Nanoparticles: Developing a Better Mechanistic Understanding Through Model Cell Membranes and in vivo Toxicity

2020 ◽  
Vol Volume 15 ◽  
pp. 4091-4104 ◽  
Author(s):  
Arek M Engstrom ◽  
Ryan A Faase ◽  
Grant W Marquart ◽  
Joe E Baio ◽  
Marilyn R Mackiewicz ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Membranes ◽  
In Vivo Toxicity ◽  
Size Dependent ◽  
Model Cell ◽  
Mechanistic Understanding

Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study

2012 ◽  
Vol 22 (39) ◽  
pp. 21305 ◽  
Author(s):  
Riccardo Marega ◽  
Linda Karmani ◽  
Lionel Flamant ◽  
Praveen Ganesh Nageswaran ◽  
Vanessa Valembois ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Cells ◽  
In Vivo Study
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