Chemical labeling strategies for small molecule natural product detection and isolation

2021 ◽  
Author(s):  
Chambers C. Hughes
Keyword(s):  
Natural Product ◽  
Small Molecule ◽  
Complex Mixtures ◽  
Untargeted Metabolomics ◽  
Chemical Labeling ◽  
Labeling Strategies

Chemical labeling enhances the analysis of complex mixtures via HPLC-MS in both targeted and untargeted metabolomics workflows.

An overview on interactions between natural product-derived β-glucan and small-molecule compounds

2021 ◽  
Vol 261 ◽  
pp. 117850
Author(s):  
Xiao-Hui Fang ◽  
Ming-Yue Zou ◽  
Fu-Quan Chen ◽  
Hui Ni ◽  
Shao-Ping Nie ◽  
...  
Keyword(s):  
Natural Product ◽  
Small Molecule

scholarly journals Biology-oriented synthesis of a natural-product inspired oxepane collection yields a small-molecule activator of the Wnt-pathway

2011 ◽  
Vol 108 (17) ◽  
pp. 6805-6810 ◽  
Author(s):  
S. Basu ◽  
B. Ellinger ◽  
S. Rizzo ◽  
C. Deraeve ◽  
M. Schurmann ◽  
...  
Keyword(s):  
Natural Product ◽  
Small Molecule ◽  
Wnt Pathway

Abstract 3429: Coordination of cell cycle repression and reduced cytoskeletal tension by the small molecule natural product, Schweinfurthin A.

2013 ◽  
Author(s):  
Thomas Turbyville ◽  
Alla Brafman ◽  
De Chen ◽  
Kathryn Romanchuck ◽  
John Beutler ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Natural Product ◽  
Small Molecule ◽  
Cytoskeletal Tension

Evaluation of chemical labeling strategies for monitoring HCV RNA using vibrational microscopy

2007 ◽  
Vol 5 (15) ◽  
pp. 2380 ◽  
Author(s):  
Matthew Noestheden ◽  
Qingyan Hu ◽  
Angela M. Tonary ◽  
Li-Lin Tay ◽  
John Paul Pezacki
Keyword(s):  
Hcv Rna ◽  
Chemical Labeling ◽  
Labeling Strategies

Ex Vivo Expansion of Hematopoietic Stem and Progenitor Cells Using a Natural Product, Garcinol

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 1174-1174
Author(s):  
Taito Nishino ◽  
Atsushi Iwama
Keyword(s):  
Stem Cell ◽  
Natural Product ◽  
Progenitor Cells ◽  
Small Molecule ◽  
Inhibitory Activity ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells ◽  
Cells Cultured

Abstract Abstract 1174 Ex vivo expansion of hematopoietic stem and progenitor cells (HSPCs) have recently been explored to optimize autologous and allogeneic HSPC transplantation and shown to be effective in the field of stem cell biology. However, to our knowledge, identification of culture conditions that allow HSPCs expansion and long-term hematopoietic reconstitution have remained incomplete, and clinical methods to expand human HSPCs have yet to be realized. In this study, we assumed that some small molecule compounds may preferentially activate signals that are required for optimal HSPC expansion and facilitate self-renewal of hematopoietic stem cells (HSCs). Thus, we evaluated the effects of several biologically active compounds on the ex vivo expansion of CD34+ hematopoietic stem and progenitor cells from human cord blood (hCB) and identified Garcinol, a plant-derived natural product as a novel modulator of HSPC proliferation. We cultured hCB CD34+ cells in serum-free medium supplemented with human thrombopoietin, human stem cell factor and Garcinol for 7 days and analyzed the cellular phenotype of the cultured cells by flow cytometry and colony assay. Although the total number of cells cultured with Garcinol was similar to those cultured without Garcinol, the cultures with Garcinol showed >2-fold increase in the number of CD34+CD38- hematopoietic stem and progenitor cells and contained 2-fold more high-proliferative-potential colony-forming cells (HPP-CFCs; >1mm in diameter) compared to control cultures. Correspondingly, SCID-repopulating cells (SRCs) were increased 2-fold during a 7-day culture with Garcinol compared to cultures without Garcinol. These findings suggest that Garcinol efficiently promotes the net expansion of HPSCs. To investigate the structure-activity relationship of Garcinol, we synthesized the chemical derivatives of Garcinol and evaluated the effect of Garcinol and its derivatives, Isogarcinol and O, O'-dimethylisogarcinol, on the proliferation of CD34+CD38- cells. Although Isogarcinol exhibited almost the same activity as Garcinol, O, O'-dimethyl isogarcinol was scarcely effective in the CD34+CD38- cell proliferation. Correspondingly, O, O'-dimethylisogarcinol had no effect on numbers of HPP-CFCs. These results indicate that dihydroxybenzoyl moiety is crucial for the positive effect of Gacinol on HSPCs.Garcinol has been reported to be a potent inhibitor of histone acetyltransferases (HAT). Thus, we estimated the HAT activity in cells treated with Garcinol and its derivatives. Garcinol and Isogarcinol inhibited HAT activity while O, O'-dimethylisogarcinol showed much less HAT inhibitory activity as compared to Garcinol and Isogarcinol, which suggested that HAT inhibitory activity of Garcinol is correlate with the expansion of HPSCs. We are now investigating gene expression profiling in cells cultured with Garcinol using DNA microarray analysis and Q-PCR. In conclusion, we have identified Garcinol, a plant-derived small-molecule compound, which exhibits inhibitory effect on HAT activity, as a novel stimulator of HSPC expansion. The results reported here indicate that Garcinol would be applied as a useful tool for the development of novel and efficient technologies for hematopoietic stem cell and gene therapies. Disclosures: No relevant conflicts of interest to declare.

Chemical labeling strategies for recognition and determination of protein and peptide

2011 ◽  
Vol 41 (4) ◽  
pp. 663-677 ◽  
Author(s):  
XiaoWen YAN ◽  
LiMin YANG ◽  
QiuQuan WANG ◽  
Ming XU
Keyword(s):  
Chemical Labeling ◽  
Labeling Strategies
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