Evaluation of chemical labeling strategies for monitoring HCV RNA using vibrational microscopy

2007 ◽  
Vol 5 (15) ◽  
pp. 2380 ◽  
Author(s):  
Matthew Noestheden ◽  
Qingyan Hu ◽  
Angela M. Tonary ◽  
Li-Lin Tay ◽  
John Paul Pezacki
Keyword(s):  
Hcv Rna ◽  
Chemical Labeling ◽  
Labeling Strategies

Chemical labeling strategies for recognition and determination of protein and peptide

2011 ◽  
Vol 41 (4) ◽  
pp. 663-677 ◽  
Author(s):  
XiaoWen YAN ◽  
LiMin YANG ◽  
QiuQuan WANG ◽  
Ming XU
Keyword(s):  
Chemical Labeling ◽  
Labeling Strategies

Chemical labeling strategies for cell biology

2006 ◽  
Vol 3 (8) ◽  
pp. 591-596 ◽  
Author(s):  
Kevin M Marks ◽  
Garry P Nolan
Keyword(s):  
Cell Biology ◽  
Chemical Labeling ◽  
Labeling Strategies

Chemical labeling strategies for small molecule natural product detection and isolation

2021 ◽  
Author(s):  
Chambers C. Hughes
Keyword(s):  
Natural Product ◽  
Small Molecule ◽  
Complex Mixtures ◽  
Untargeted Metabolomics ◽  
Chemical Labeling ◽  
Labeling Strategies

Chemical labeling enhances the analysis of complex mixtures via HPLC-MS in both targeted and untargeted metabolomics workflows.

Hepatitis G Viral RNA in Serum and in Peripheral Blood Mononuclear Cells and Its Relation to HCV-RNA in Patients with Clotting Disorders

1997 ◽  
Vol 77 (05) ◽  
pp. 0868-0872 ◽  
Author(s):  
Li Sheng ◽  
Ann Soumillion ◽  
Kathelijne Peerlinck ◽  
Chris Verslype ◽  
Lan Lin ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Elevated Serum ◽  
Normal Serum ◽  
Hcv Rna ◽  
Peripheral Blood Mononuclear ◽  
Hepatitis G Virus ◽  
Blood Mononuclear Cells ◽  
Hepatitis G

SummaryThe hepatitis G virus (HGV) has recently been identified as a new member of the Flaviviridae family. Infection by this virus is thought to be associated with blood borne hepatitis. In this study, the presence of HCV- and HGV-RNAs in serum or plasma (175 patients) and in peripheral blood mononuclear cells (PBMC) (133 patients) was investigated in patients with clotting disorders using a sensitive reverse transcriptase polymerase chain reaction (RT-PCR). HGV-RNA was detected in serum of 26 patients (14.8%). In apparently healthy blood donors, serum HGV-RNA was detected in 4 of 358 individuals investigated (1.12%). Ninety two percent of the 26 serum HGV-RNA positive patients had coinfection with the hepatitis C virus (HGV), especially with HCV genotype lb, the most common genotype in Belgium. Of these coinfected patients, 15 (62.5%) showed elevated serum ALT levels. Two patients who were solely infected with HGV had normal serum ALT. HGV-RNA in PBMC was found in 18 patients, of whom 3 were negative for serum HGV-RNA. As in case of HCV, HGV-RNA in PBMC is preferentially sensitive to interferon treatment. Nevertheless, rapid reappearance of HGV-RNA in PBMC was observed after cessation of treatment. In one patient, persistent serum ALT elevation seems to be associated with continued HGV viremia, despite the disappearance of serum HCV-RNA.

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