Upconversion nanoparticles-labelled immunochromatographic assay for quantitative biosensing

2020 ◽  
Vol 44 (36) ◽  
pp. 15498-15506
Author(s):  
Fengping Hou ◽  
Haiyun Liu ◽  
Yun Zhang ◽  
Zhendong Gao ◽  
Shiqi Sun ◽  
...  

Quantitative determination of FMDV antibody using immunochromatographic strips with high sensitivity and specificity was achieved within 20 minutes.

Fire Safety ◽  
2018 ◽  
pp. 74-79
Author(s):  
O. Scherbyna

The methods of isolation, purification and qualitative and quantitative determination of phenol in air and biological fluids of an organism are offered. Isolation was carried out by distillation with water vapor, purification by extraction with chloroform, and identification by qualitative reactions, by chromatography in a thin layer of sorbent and by liquid chromatography. The quantitative determination of phenol was carried out by liquid chromatography in a reverse phase (chromatograph "Tsvet-304" with an ultraviolet detector). Calculation of the quantitative content of phenol was carried out by the method of absolute calibration. As a result of the research, it was found that by using the molecular liquid chromatography method, it is possible to determine 17-20% of phenol isolated from urine and 21-24% isolated from the air. The retention time of phenol 1h.14s, the results of the analysis are well reproducible. The worked out techniques have high resolution, high sensitivity and speed of analysis.


RSC Advances ◽  
2014 ◽  
Vol 4 (93) ◽  
pp. 51315-51320 ◽  
Author(s):  
Xiang Lin ◽  
Wu-Li-Ji Hasi ◽  
Xiu-Tao Lou ◽  
Shuang Lin ◽  
Fang Yang ◽  
...  

Trace melamine can be quantitatively detected without any sample pretreatment using a new SERS measurement configuration.


2021 ◽  
Vol 22 (4) ◽  
pp. 1842
Author(s):  
Dmitry Gribanyov ◽  
Gleb Zhdanov ◽  
Andrei Olenin ◽  
Georgii Lisichkin ◽  
Alexandra Gambaryan ◽  
...  

Development of sensitive techniques for rapid detection of viruses is on a high demand. Surface-enhanced Raman spectroscopy (SERS) is an appropriate tool for new techniques due to its high sensitivity. DNA aptamers are short structured oligonucleotides that can provide specificity for SERS biosensors. Existing SERS-based aptasensors for rapid virus detection had several disadvantages. Some of them lacked possibility of quantitative determination, while others had sophisticated and expensive implementation. In this paper, we provide a new approach that combines rapid specific detection and the possibility of quantitative determination of viruses using the example of influenza A virus.


2021 ◽  
Vol 113 ◽  
pp. 103225
Author(s):  
Lei Zhang ◽  
Yingshan Chen ◽  
Qin Zhu ◽  
Wenjin Ji ◽  
Suqing Zhao

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Raziyeh Behzadmehr ◽  
Khadije Rezaie Keikhaie ◽  
Rehaneh Behzadmehr

Background: Early determination of fetal gender during pregnancy is essential for the early detection of gender-linked diseases in the fetus. Thus, the purpose of this study was to evaluate the sensitivity and specificity of ultrasonography in determining fetal gender in pregnant mothers at 11 to 14 weeks of gestation. Methods: The study included 227 pregnant mothers at 11 to 14 weeks of gestational age. Ultrasonography results were recorded for fetal gender determination based on gestational age and body mass index (BMI). Results: The sensitivity and specificity of ultrasonography for male gender determination were 91.73% and 99.05%, respectively. This value for female gender determination was 99.05% and 91.73%, respectively. Conclusions: The results of our study showed that ultrasonography at 11 to 14 weeks of gestation had high sensitivity and specificity in detecting gender, and its sensitivity in female gender determination was higher.


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