Proteomic analysis of Listeria monocytogenes exposed to free and nanostructured antimicrobial lipopeptides

2021 ◽  
Author(s):  
Paolo Stincone ◽  
Carolina Comerlato ◽  
A. Brandelli
Keyword(s):  
Listeria Monocytogenes ◽  
Proteomic Analysis ◽  
Antimicrobial Lipopeptide ◽  
Proteomics Approach ◽  
Sublethal Doses ◽  
Antimicrobial Lipopeptides ◽  
First Time

In this work, the effect of antimicrobial lipopeptide P34 on Listeria monocytogenes was evaluated for the first time through a proteomics approach. Bacteria were treated with sublethal doses of peptide...

Control of Listeria monocytogenes biofilm by paenibacterin, a natural antimicrobial lipopeptide

Food Control ◽  
2018 ◽  
Vol 84 ◽  
pp. 529-535 ◽  
Author(s):  
Rui Li ◽  
Wenfang Du ◽  
Jun Yang ◽  
Zhiguo Liu ◽  
Ahmed E. Yousef
Keyword(s):  
Listeria Monocytogenes ◽  
Natural Antimicrobial ◽  
Antimicrobial Lipopeptide

scholarly journals Global Proteomic Analysis of Listeria monocytogenes’ Response to Linalool

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2449
Author(s):  
Zhipeng Gao ◽  
Weiming Zhong ◽  
Ting Liu ◽  
Tianyu Zhao ◽  
Jiajing Guo
Keyword(s):  
Listeria Monocytogenes ◽  
Foodborne Pathogens ◽  
Proteomic Analysis ◽  
Drug Targets ◽  
Enrichment Analysis ◽  
Good Evidence ◽  
New Drugs ◽  
Organic System ◽  
Flow Cytometry Data ◽  
Theoretical Support

Listeria monocytogenes (LM) is one of the most serious foodborne pathogens. Listeriosis, the disease caused by LM infection, has drawn attention worldwide because of its high hospitalization and mortality rates. Linalool is a vital constituent found in many essential oils; our previous studies have proved that linalool exhibits strong anti-Listeria activity. In this study, iTRAQ-based quantitative proteomics analysis was performed to explore the response of LM exposed to linalool, and to unravel the mode of action and drug targets of linalool against LM. A total of 445 differentially expressed proteins (DEPs) were screened out, including 211 up-regulated and 234 down-regulated proteins which participated in different biological functions and pathways. Thirty-one significantly enriched gene ontology (GO) functional categories were obtained, including 12 categories in “Biological Process”, 10 categories in “Cell Component”, and 9 categories in “Molecular Function”. Sixty significantly enriched biological pathways were classified, including 6 pathways in “Cell Process”, 6 pathways in “Environmental Information Processing”, 3 pathways in “Human Disease”, 40 pathways in “Metabolism”, and 2 pathways in “Organic System”. GO and Kyoto Encyclopedia of Genes (KEGG) enrichment analysis together with flow cytometry data implied that cell membranes, cell walls, nucleoids, and ribosomes might be the targets of linalool against LM. Our study provides good evidence for the proteomic analysis of bacteria, especially LM, exposed to antibacterial agents. Further, those drug targets discovered by proteomic analysis can provide theoretical support for the development of new drugs against LM.

Contrasting regulation of macrophage iron homeostasis in response to infection with Listeria monocytogenes depending on localization of bacteria

Metallomics ◽  
2015 ◽  
Vol 7 (6) ◽  
pp. 1036-1045 ◽  
Author(s):  
David Haschka ◽  
Manfred Nairz ◽  
Egon Demetz ◽  
Sebastian Wienerroither ◽  
Thomas Decker ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Iron Homeostasis ◽  
First Time

We describe for the first time the changes of iron homeostasis in response to infection of macrophages withListeria monocytogenes.

scholarly journals Changes in the Salivary Proteome Associated With Canine Pyometra

2020 ◽  
Vol 7 ◽  
Author(s):  
Lorena Franco-Martínez ◽  
Anita Horvatić ◽  
Andrea Gelemanović ◽  
Marko Samardžija ◽  
Vladimir Mrljak ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Calcium Binding ◽  
Acute Phase Proteins ◽  
Future Studies ◽  
Non Invasive ◽  
Proinflammatory Mediators ◽  
Time Changes ◽  
Classification Tool ◽  
First Time ◽  
Saliva Composition

The present study evaluated for the first time changes in the saliva proteome in bitches with pyometra through a high-throughput quantitative proteomic analysis. The aims were to explore whether saliva composition could reflect the physiopathological changes occurring in canine pyometra and to identify potential biomarkers of the disease. Saliva samples from six healthy (H) and six bitches with pyometra (P) were analyzed using tandem mass tags–based approach. Additionally, 15 samples were used for the validation of changes in haptoglobin (Hp) concentration in saliva of dogs with pyometra. Proteomic analysis quantified 707 proteins in saliva. Comparison of the two groups revealed 16 unique proteins significantly modulated in saliva, with S100A calcium-binding protein 12 (S100A12), vimentin, and Hp the most up-regulated in canine pyometra. According to PANTHER (Protein Analysis Through Evolutionary Relationships) classification tool, these proteins are mainly related to proinflammatory mediators, acute-phase proteins, and sepsis. In conclusion, it can be stated that there are changes in various proteins in saliva in canine pyometra reflecting different physiopathological changes occurring in this disease. These proteins could be a source of potential non-invasive biomarkers for this disease that should be confirmed in future studies.

scholarly journals Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S

2008 ◽  
Vol 8 (1) ◽  
pp. 102 ◽  
Author(s):  
Efstathios S Giotis ◽  
Arunachalam Muthaiyan ◽  
Ian S Blair ◽  
Brian J Wilkinson ◽  
David A McDowell
Keyword(s):  
Listeria Monocytogenes ◽  
Proteomic Analysis ◽  
Tolerance Response ◽  
Alkali Tolerance

scholarly journals First Report on the Finding of Listeria mnocytogenes ST121 Strain in a Dolphin Brain

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 802
Author(s):  
Yann Sévellec ◽  
Marina Torresi ◽  
Benjamin Félix ◽  
Féderica Palma ◽  
Gabriella Centorotola ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Mediterranean Sea ◽  
Food Production ◽  
Polychlorinated Biphenyl ◽  
Foodborne Illness ◽  
Production Environment ◽  
Related Strain ◽  
Genomic Features ◽  
First Time ◽  
Production Industry

Listeria monocytogenes (Lm) is a ubiquitous bacterium that causes the foodborne illness, listeriosis. Clonal complexes (CC), such as CC121, are overrepresented in the food production industry, and are rarely reported in animals and the environment. Working within a European-wide project, we investigated the routes by which strains are transmitted from environments and animals to food and the food production environment (FPE). In this context, we report, for the first time, the occurrence of a ST121 (CC121) strain isolated from a dolphin brain. The genome was compared with the genomes of 376 CC121 strains. Genomic comparisons showed that 16 strains isolated from food were the closest to the dolphin strain. Like most of the food strains analyzed here, the dolphin strain included genomic features (transposon Tn6188, plasmid pLM6179), both described as being associated with the strain’s adaptation to the FPE. Like all 376 strains, the dolphin strain contained a truncated actA gene and inlA gene, both described as being associated with attenuated virulence. Despite this fact, the strain was able to cross blood-brain barrier in immunosuppressed dolphin exposed polychlorinated biphenyl and invaded by parasites. Our data suggest that the dolphin was infected by a food-related strain released into the Mediterranean Sea.

scholarly journals Proteomic analysis of desmosomes reveals novel components required for epidermal integrity

2020 ◽  
Vol 31 (11) ◽  
pp. 1140-1153
Author(s):  
Kwabena A. Badu-Nkansah ◽  
Terry Lechler
Keyword(s):  
Cell Adhesion ◽  
Proteomic Analysis ◽  
Neonatal Lethality ◽  
Proteomics Approach ◽  
Associated Proteins ◽  
Cell Cell

Desmosomes are cell-cell adhesion structures that are required for the integrity of the skin and heart. Here, we have used a proteomics approach to identify novel desmosome-associated proteins. Ablation of Crk and Crkl, two such proteins identified, results in desmosome defects, epidermal fragility and neonatal lethality.

Proteomic analysis of the response of Listeria monocytogenes to bile salts under anaerobic conditions

2013 ◽  
Vol 62 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Angela Payne ◽  
Ty B. Schmidt ◽  
Bindu Nanduri ◽  
Ken Pendarvis ◽  
Joseph R. Pittman ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Proteomic Analysis ◽  
Bile Salts ◽  
Anaerobic Conditions

Comparative proteomic analysis of Listeria monocytogenes tolerance to bile stress

2012 ◽  
Vol 63 (1) ◽  
pp. 117-128 ◽  
Author(s):  
Qingchun Zhou ◽  
Qiang Zhang ◽  
Huiping Qu ◽  
Li Wang ◽  
Feifei Feng ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis
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