Contrasting regulation of macrophage iron homeostasis in response to infection with Listeria monocytogenes depending on localization of bacteria

Metallomics ◽  
2015 ◽  
Vol 7 (6) ◽  
pp. 1036-1045 ◽  
Author(s):  
David Haschka ◽  
Manfred Nairz ◽  
Egon Demetz ◽  
Sebastian Wienerroither ◽  
Thomas Decker ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Iron Homeostasis ◽  
First Time

We describe for the first time the changes of iron homeostasis in response to infection of macrophages withListeria monocytogenes.

Regulation of macrophage iron homeostasis is associated with the localization of bacteria

Metallomics ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 454-461 ◽  
Author(s):  
Zhenshun Gan ◽  
Xueyou Tang ◽  
Zhenjie Wang ◽  
Jiahui Li ◽  
Zhen Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Iron Homeostasis ◽  
Intracellular Bacteria ◽  
First Time

We describe, for the first time, the changes of iron homeostasis in response to the infection of macrophages with extracellular bacteriaEscherichia coliK88 and intracellular bacteriaSalmonella typhimurium.

scholarly journals Lipopolysaccharide Induces a Significant Increase in Expression of Iron Regulatory Hormone Hepcidin in the Cortex and Substantia Nigra in Rat Brain

Endocrinology ◽  
2008 ◽  
Vol 149 (8) ◽  
pp. 3920-3925 ◽  
Author(s):  
Qin Wang ◽  
Fang Du ◽  
Zhong-Ming Qian ◽  
Xiao Hu Ge ◽  
Li Zhu ◽  
...  
Keyword(s):  
Substantia Nigra ◽  
Iron Homeostasis ◽  
Brain Regions ◽  
Iron Regulation ◽  
Rt Pcr ◽  
Immunofluorescence Analysis ◽  
Specific Regulation ◽  
First Time ◽  
The Brain ◽  
Hepcidin Mrna

Hepcidin plays an essential role in maintaining normal iron homeostasis outside the brain. This recently discovered iron regulation hormone is predominantly expressed in the liver, and regulated by iron and hypoxia. As an antimicrobial peptide, this hormone is also elevated during infections and inflammation. In this study we investigated the expression of hepcidin mRNA and protein in different brain regions, including the cortex, hippocampus, striatum, and substantia nigra, and the effects of lipopolysaccharide (LPS) on the expression of hepcidin using quantitative real-time RT-PCR and immunofluorescence analysis. Our data provided further evidence for the existence of hepcidin in all the regions we examined. We also demonstrated for the first time that LPS administration by iv injection can regulate the expression of hepcidin mRNA and protein not only in peripheral organs such as the liver, but also in the brain. LPS induced a significant increase in the expression of hepcidin mRNA and protein in the cortex and substantia nigra, but not in the hippocampus and striatum, indicating a regionally specific regulation of LPS on hepcidin in the brain. The relevant mechanisms and the functions of hepcidin in the brain remain to be elucidated.

scholarly journals Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols

Marine Drugs ◽  
2021 ◽  
Vol 19 (8) ◽  
pp. 459
Author(s):  
Gennaro Riccio ◽  
Genoveffa Nuzzo ◽  
Gianluca Zazo ◽  
Daniela Coppola ◽  
Giuseppina Senese ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Lipid Peroxidation ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Hepg2 Cells ◽  
Iron Homeostasis ◽  
Protein Levels ◽  
Antarctic Research ◽  
First Time ◽  
Antarctic Sponges

Sponges are known to produce a series of compounds with bioactivities useful for human health. This study was conducted on four sponges collected in the framework of the XXXIV Italian National Antarctic Research Program (PNRA) in November-December 2018, i.e., Mycale (Oxymycale) acerata, Haliclona (Rhizoniera) dancoi, Hemimycale topsenti, and Hemigellius pilosus. Sponge extracts were fractioned and tested against hepatocellular carcinoma (HepG2), lung carcinoma (A549), and melanoma cells (A2058), in order to screen for antiproliferative or cytotoxic activity. Two different chemical classes of compounds, belonging to mycalols and suberitenones, were identified in the active fractions. Mycalols were the most active compounds, and their mechanism of action was also investigated at the gene and protein levels in HepG2 cells. Of the differentially expressed genes, ULK1 and GALNT5 were the most down-regulated genes, while MAPK8 was one of the most up-regulated genes. These genes were previously associated with ferroptosis, a programmed cell death triggered by iron-dependent lipid peroxidation, confirmed at the protein level by the down-regulation of GPX4, a key regulator of ferroptosis, and the up-regulation of NCOA4, involved in iron homeostasis. These data suggest, for the first time, that mycalols act by triggering ferroptosis in HepG2 cells.

scholarly journals First Report on the Finding of Listeria mnocytogenes ST121 Strain in a Dolphin Brain

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 802
Author(s):  
Yann Sévellec ◽  
Marina Torresi ◽  
Benjamin Félix ◽  
Féderica Palma ◽  
Gabriella Centorotola ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Mediterranean Sea ◽  
Food Production ◽  
Polychlorinated Biphenyl ◽  
Foodborne Illness ◽  
Production Environment ◽  
Related Strain ◽  
Genomic Features ◽  
First Time ◽  
Production Industry

Listeria monocytogenes (Lm) is a ubiquitous bacterium that causes the foodborne illness, listeriosis. Clonal complexes (CC), such as CC121, are overrepresented in the food production industry, and are rarely reported in animals and the environment. Working within a European-wide project, we investigated the routes by which strains are transmitted from environments and animals to food and the food production environment (FPE). In this context, we report, for the first time, the occurrence of a ST121 (CC121) strain isolated from a dolphin brain. The genome was compared with the genomes of 376 CC121 strains. Genomic comparisons showed that 16 strains isolated from food were the closest to the dolphin strain. Like most of the food strains analyzed here, the dolphin strain included genomic features (transposon Tn6188, plasmid pLM6179), both described as being associated with the strain’s adaptation to the FPE. Like all 376 strains, the dolphin strain contained a truncated actA gene and inlA gene, both described as being associated with attenuated virulence. Despite this fact, the strain was able to cross blood-brain barrier in immunosuppressed dolphin exposed polychlorinated biphenyl and invaded by parasites. Our data suggest that the dolphin was infected by a food-related strain released into the Mediterranean Sea.

scholarly journals Hepatic Iron Overload following Liver Transplantation from a C282Y/H63D Compound Heterozygous Donor

2018 ◽  
Vol 2018 ◽  
pp. 1-4 ◽  
Author(s):  
E. Veitsman ◽  
E. Pras ◽  
O. Pappo ◽  
A. Arish ◽  
R. Eshkenazi ◽  
...  
Keyword(s):  
Iron Overload ◽  
Genetic Disease ◽  
Sclerosing Cholangitis ◽  
Iron Homeostasis ◽  
Hereditary Hemochromatosis ◽  
Compound Heterozygous ◽  
Primary Role ◽  
Hepatic Iron Overload ◽  
Liver Biopsies ◽  
First Time

Hereditary hemochromatosis (HH) is a genetic disease associated with progressive iron overload, eventually leading in some cases to damage of parenchymal organs, such as the liver, pancreas, and heart. Although the gene had been identified (HFE), HH pathogenesis remains to be fully elucidated. We report here, for the first time, a case of inadvertent transplantation of a liver from a donor with C282Y/H63D compound heterozygosity into a nonhemochromatotic 19-year-old Caucasian male recipient with primary sclerosing cholangitis. Progressive iron overload occurred over 1.5 years, as observed in liver biopsies and iron studies, after ruling out secondary causes of iron overload. This case strengthens the hypothesis that the liver, rather than the small intestine, plays a primary role in the maintenance of iron homeostasis.

scholarly journals AsnB Mediates Amidation of Meso-Diaminopimelic Acid Residues in the Peptidoglycan of Listeria monocytogenes and Affects Bacterial Surface Properties and Host Cell Invasion

2021 ◽  
Vol 12 ◽  
Author(s):  
Lei Sun ◽  
Gil Rogiers ◽  
Pascal Courtin ◽  
Marie-Pierre Chapot-Chartier ◽  
Hélène Bierne ◽  
...  
Keyword(s):  
Cell Wall ◽  
Listeria Monocytogenes ◽  
Cell Surface ◽  
Bacterial Species ◽  
Functional Characterization ◽  
Diaminopimelic Acid ◽  
Bacterial Surface ◽  
Gram Positive Bacteria ◽  
First Time

A mutant of Listeria monocytogenes ScottA with a transposon in the 5' untranslated region of the asnB gene was identified to be hypersensitive to the antimicrobial t-cinnamaldehyde. Here, we report the functional characterization of AsnB in peptidoglycan (PG) modification and intracellular infection. While AsnB of Listeria is annotated as a glutamine-dependent asparagine synthase, sequence alignment showed that this protein is closely related to a subset of homologs that catalyze the amidation of meso-diaminopimelic acid (mDAP) residues in the peptidoglycan of other bacterial species. Structural analysis of peptidoglycan from an asnB mutant, compared to that of isogenic wild-type (WT) and complemented mutant strains, confirmed that AsnB mediates mDAP amidation in L. monocytogenes. Deficiency in mDAP amidation caused several peptidoglycan- and cell surface-related phenotypes in the asnB mutant, including formation of shorter but thicker cells, susceptibility to lysozyme, loss of flagellation and motility, and a strong reduction in biofilm formation. In addition, the mutant showed reduced invasion of human epithelial JEG-3 and Caco-2 cells. Analysis by immunofluorescence microscopy revealed that asnB inactivation abrogated the proper display at the listerial surface of the invasion protein InlA, which normally gets cross-linked to mDAP via its LPXTG motif. Together, this work shows that AsnB of L. monocytogenes, like several of its homologs in related Gram-positive bacteria, mediates the amidation of mDAP residues in the peptidoglycan and, in this way, affects several cell wall and cell surface-related properties. It also for the first time implicates the amidation of peptidoglycan mDAP residues in cell wall anchoring of InlA and in bacterial virulence.

scholarly journals Chitin Attenuates Expression of Listeria monocytogenes Virulence Genes in vitro

2020 ◽  
Vol 11 ◽  
Author(s):  
Miguel Villoria Recio ◽  
Bo-Hyung Lee ◽  
Eva Maria Sternkopf Lillebæk ◽  
Birgitte H. Kallipolitis ◽  
Cormac G. M. Gahan ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Disease Risk ◽  
Foodborne Pathogen ◽  
Post Translational Modification ◽  
Competitive Environments ◽  
Virulence Potential ◽  
Alternative Approach ◽  
External Signals ◽  
First Time

External signals are crucial for bacteria to sense their immediate environment and fine-tune gene expression accordingly. The foodborne pathogen Listeria monocytogenes senses a range of environmental cues in order to activate or deactivate the virulence-inducing transcriptional factor PrfA during transition between infectious and saprophytic lifecycles. Chitin is an abundant biopolymer formed from linked β-(1–4)-N-acetyl-D-glucosamine residues associated with fungi, the exoskeleton of insects and often incorporated into foods as a thickener or stabilizer. L. monocytogenes evolved to hydrolyse chitin, presumably, to facilitate nutrient acquisition from competitive environments such as soil where the polymer is abundant. Since mammals do not produce chitin, we reasoned that the polymer could serve as an environmental signal contributing to repression of L. monocytogenes PrfA-dependent expression. This study shows a significant downregulation of the core PrfA-regulon during virulence-inducing conditions in vitro in the presence of chitin. Our data suggest this phenomenon occurs through a mechanism that differs from PTS-transport of oligosaccharides generated from either degradation or chitinase-mediated hydrolysis of the polymer. Importantly, an indication that chitin can repress virulence expression of a constitutively active PrfA∗ mutant is shown, possibly mediated via a post-translational modification inhibiting PrfA∗ activity. To our knowledge, this is the first time that chitin is reported as a molecule with anti-virulence properties against a pathogenic bacterium. Thus, our findings identify chitin as a signal which may downregulate the virulence potential of the pathogen and may provide an alternative approach toward reducing disease risk.

scholarly journals Macroalgal–bacterial interactions: identification and role of thallusin in morphogenesis of the seaweed Ulva (Chlorophyta)

2020 ◽  
Vol 71 (11) ◽  
pp. 3340-3349 ◽  
Author(s):  
Taghreed Alsufyani ◽  
Gianmaria Califano ◽  
Michael Deicke ◽  
Jan Grueneberg ◽  
Anne Weiss ◽  
...  
Keyword(s):  
Cell Wall ◽  
Iron Homeostasis ◽  
Solid Phase ◽  
Algal Growth ◽  
Iron Complex ◽  
Intracellular Iron ◽  
Axenic Conditions ◽  
New Applications ◽  
Ecological Reconstruction ◽  
First Time

Abstract Macroalgal microbiomes have core functions related to biofilm formation, growth, and morphogenesis of seaweeds. In particular, the growth and development of the sea lettuce Ulva spp. (Chlorophyta) depend on bacteria releasing morphogenetic compounds. Under axenic conditions, the macroalga Ulva mutabilis develops a callus-like phenotype with cell wall protrusions. However, co-culturing with Roseovarius sp. (MS2) and Maribacter sp. (MS6), which produce various stimulatory chemical mediators, completely recovers morphogenesis. This ecological reconstruction forms a tripartite community which can be further studied for its role in cross-kingdom interactions. Hence, our study sought to identify algal growth- and morphogenesis-promoting factors (AGMPFs) capable of phenocopying the activity of Maribacter spp. We performed bioassay-guided solid-phase extraction in water samples collected from U. mutabilis aquaculture systems. We uncovered novel ecophysiological functions of thallusin, a sesquiterpenoid morphogen, identified for the first time in algal aquaculture. Thallusin, released by Maribacter sp., induced rhizoid and cell wall formation at a concentration of 11 pmol l−1. We demonstrated that gametes acquired the iron complex of thallusin, thereby linking morphogenetic processes with intracellular iron homeostasis. Understanding macroalgae–bacteria interactions permits further elucidation of the evolution of multicellularity and cellular differentiation, and development of new applications in microbiome-mediated aquaculture systems.

scholarly journals Dual Transcriptional Profile of Aspergillus flavus during Co-Culture with Listeria monocytogenes and Aflatoxin B1 Production: A Pathogen–Pathogen Interaction

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 198
Author(s):  
Iliada K. Lappa ◽  
Angeliki Maria Dionysopoulou ◽  
Spiros Paramithiotis ◽  
Maria Georgiadou ◽  
Eleftherios H. Drosinos
Keyword(s):  
Listeria Monocytogenes ◽  
Aspergillus Flavus ◽  
Aflatoxin B1 ◽  
Transcriptional Profile ◽  
Malt Extract ◽  
Natural Ecosystems ◽  
Complex Dynamic ◽  
Virulence Potential ◽  
First Time ◽  
Insight Into

The objective of this study was to investigate the effect of growth temperature and co-culture of Aspergillus flavus with Listeria monocytogenes on the production of Aflatoxin B1 (AFB1) and the transcriptional profile of associated regulatory and biosynthetic genes. The transcription of virulence- and homeostasis-associated genes of L. monocytogenes was also assessed. For this purpose, mono- and co-cultures of L. monocytogenes strain LQC 15257 and A. flavus strain 18.4 were inoculated into Malt Extract broth and allowed to grow for seven days at 25 °C and 30 °C. AFB1 quantification was performed by HPLC analysis and gene expression assessment by RT-qPCR. AFB1 production was lower at 30 °C compared to 25 °C during monoculture and also lower during co-cultures at both temperatures. This was accompanied by downregulation of aflM, aflR, aflP, and aflS during monoculture and aflM and aflS during co-culture at 30 °C. On the other hand, transcription of prfA, plcA, plcB, inlA, inlB, inlJ, murE, accA, acpP, as well as fapR, was not affected. sigB gene was downregulated after co-culture with the fungus at 25 °C and hly was downregulated after monoculture at 30 °C compared to 25 °C. In this work, the molecular interactions between A. flavus and L. monocytogenes were studied for the first time, offering a novel insight into their co-occurrence. Monitoring of their toxigenic and virulence potential at the molecular level revealed a complex dynamic in natural ecosystems.

Proteomic analysis of Listeria monocytogenes exposed to free and nanostructured antimicrobial lipopeptides

2021 ◽  
Author(s):  
Paolo Stincone ◽  
Carolina Comerlato ◽  
A. Brandelli
Keyword(s):  
Listeria Monocytogenes ◽  
Proteomic Analysis ◽  
Antimicrobial Lipopeptide ◽  
Proteomics Approach ◽  
Sublethal Doses ◽  
Antimicrobial Lipopeptides ◽  
First Time

In this work, the effect of antimicrobial lipopeptide P34 on Listeria monocytogenes was evaluated for the first time through a proteomics approach. Bacteria were treated with sublethal doses of peptide...

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