scholarly journals Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in vivo optical imaging

2020 ◽  
Vol 1 (5) ◽  
pp. 967-987 ◽  
Author(s):  
Dhermendra K. Tiwari ◽  
Manisha Tiwari ◽  
Takashi Jin
Keyword(s):  
High Resolution ◽  
Optical Imaging ◽  
Recent Progress ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Imaging Techniques ◽  
Infrared Fluorescent Protein ◽  
In Vivo Optical Imaging

This review presents the recent progress on NIR fluorescent protein and bioluminescence-based probes with high-resolution in vivo imaging techniques.

scholarly journals Quantitative in vivo bioluminescence imaging of orthotopic patient-derived glioblastoma xenografts

2020 ◽  
Author(s):  
Anna L. Koessinger ◽  
Dominik Koessinger ◽  
Katrina Stevenson ◽  
Catherine Cloix ◽  
Louise Mitchell ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Growth ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Imaging Techniques ◽  
Glioblastoma Cells ◽  
Non Invasive ◽  
In Vivo Bioluminescence Imaging ◽  
Infrared Fluorescent Protein

AbstractDespite extensive research, little progress has been made in glioblastoma therapy, owing in part to a lack of adequate preclinical in vivo models to study this disease. To mitigate this, primary patient-derived cell lines, which maintain their specific stem-like phenotypes, have replaced established glioblastoma cell lines. However, due to heterogenous tumour growth inherent in glioblastoma, the use of primary cells for orthotopic in vivo studies often requires large experimental group sizes. Therefore, when using intracranial patient-derived xenograft (PDX) approaches, it is advantageous to deploy imaging techniques to monitor tumour growth and allow stratification of mice. Here we show that stable expression of near-infrared fluorescent protein (iRFP) in patient-derived glioblastoma cells enables rapid direct non-invasive monitoring of tumour development without compromising tumour stemness or tumorigenicity. Moreover, as this approach does not depend on the use of agents like luciferin, which can cause variability due to changing bioavailability, it can be used for quantitative longitudinal monitoring of tumour growth. Notably, we show that this technique also allows quantitative assessment of tumour burden in highly invasive models spreading throughout the brain. Thus, iRFP transduction of primary patient-derived glioblastoma cells is a reliable, cost- and time-effective way to monitor heterogenous orthotopic PDX growth.

scholarly journals Role of Fluorophore Charge on the In Vivo Optical Imaging Properties of Near-Infrared Cyanine Dye/Monoclonal Antibody Conjugates

2015 ◽  
Vol 27 (2) ◽  
pp. 404-413 ◽  
Author(s):  
Kazuhide Sato ◽  
Alexander P. Gorka ◽  
Tadanobu Nagaya ◽  
Megan S. Michie ◽  
Roger R. Nani ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Optical Imaging ◽  
Near Infrared ◽  
Cyanine Dye ◽  
Antibody Conjugates ◽  
Imaging Properties ◽  
In Vivo Optical Imaging

scholarly journals Bright and stable near-infrared Pluronic–silica nanoparticles as contrast agents for in vivo optical imaging

2016 ◽  
Vol 4 (33) ◽  
pp. 5560-5566 ◽  
Author(s):  
Lesan Yan ◽  
Huiquan Wang ◽  
Anqi Zhang ◽  
Calvin Zhao ◽  
Yongping Chen ◽  
...  
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Contrast Agents ◽  
Optical Imaging ◽  
Silica Nanoparticles ◽  
Near Infrared ◽  
Circulation Time ◽  
In Vivo Optical Imaging

The IR780@NPs exhibited excellent characteristics for in vivo imaging with a long circulation time and high retention in tumor and sentinel lymph node.

scholarly journals Near-Infrared Fluorescent Nanoprobes for in Vivo Optical Imaging

Nanomaterials ◽  
2012 ◽  
Vol 2 (2) ◽  
pp. 92-112 ◽  
Author(s):  
Chai-Hoon Quek ◽  
Kam W. Leong
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
In Vivo Optical Imaging

scholarly journals Near-infrared-emissive polymersomes: Self-assembled soft matter for in vivo optical imaging

2005 ◽  
Vol 102 (8) ◽  
pp. 2922-2927 ◽  
Author(s):  
P. P. Ghoroghchian ◽  
P. R. Frail ◽  
K. Susumu ◽  
D. Blessington ◽  
A. K. Brannan ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Soft Matter ◽  
Near Infrared ◽  
Self Assembled ◽  
In Vivo Optical Imaging

Synthesis and evaluation of curcumin-based near-infrared fluorescent probes for the in vivo optical imaging of amyloid-β plaques

2021 ◽  
pp. 105167
Author(s):  
Yong Dae Park ◽  
Mayank Kinger ◽  
Changho Min ◽  
Sang Yeob Lee ◽  
Youngjoo Byun ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Amyloid Β ◽  
In Vivo Optical Imaging

scholarly journals Quantitative assessment of near-infrared fluorescent proteins

2021 ◽  
Author(s):  
Kiryl Piatkevich ◽  
Hanbin Zhang ◽  
Stavrini Papadaki ◽  
Xiaoting Sun ◽  
Luxia Yao ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Quantitative Assessment ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Oligomeric State ◽  
C Elegans ◽  
The Right ◽  
Infrared Fluorescent Protein

Abstract Recent progress in fluorescent protein development has generated a large diversity of near-infrared fluorescent proteins, which are rapidly becoming popular probes for a variety of imaging applications. To assist end-users with a selection of the right near-infrared fluorescent protein for a given application, we will conduct a quantitative assessment of intracellular brightness, photostability, and oligomeric state of 19 near-infrared fluorescent proteins in cultured mammalian cells. The top-performing proteins will be further validated for in vivo imaging of neurons in C. elegans, zebrafish, and mice. We will also assess the applicability of the selected NIR FPs for expansion microscopy and two-photon imaging.

Synthesis of chitosan-coated near-infrared layered double hydroxide nanoparticles for in vivo optical imaging

2012 ◽  
Vol 22 (12) ◽  
pp. 5503 ◽  
Author(s):  
Pei-Ru Wei ◽  
Shih-Hsun Cheng ◽  
Wei-Neng Liao ◽  
Kun-Che Kao ◽  
Ching-Feng Weng ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Layered Double Hydroxide ◽  
Near Infrared ◽  
Double Hydroxide ◽  
In Vivo Optical Imaging ◽  
Layered Double Hydroxide Nanoparticles

scholarly journals Photoacoustic imaging of the near-infrared fluorescent protein iRFP in vivo

2012 ◽  
Author(s):  
Arie Krumholz ◽  
Grigory S. Filonov ◽  
Jun Xia ◽  
Junjie Yao ◽  
Vladislav V. Verkhusha ◽  
...  
Keyword(s):  
Near Infrared ◽  
Fluorescent Protein ◽  
Photoacoustic Imaging ◽  
Infrared Fluorescent Protein
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