scholarly journals Metachronal actuation of microscopic magnetic artificial cilia generates strong microfluidic pumping

Lab on a Chip ◽  
2020 ◽  
Vol 20 (19) ◽  
pp. 3569-3581
Author(s):  
Shuaizhong Zhang ◽  
Zhiwei Cui ◽  
Ye Wang ◽  
Jaap M. J. den Toonder
Keyword(s):  
Microfluidic Flow ◽  
Artificial Cilia ◽  
Microfluidic Pumping

Microscopic magnetic artificial cilia (μMAC) performing metachronal motion are experimentally demonstrated to generate unprecedented strong microfluidic flow.

A continuous roll-pulling approach for the fabrication of magnetic artificial cilia with microfluidic pumping capability

Lab on a Chip ◽  
2016 ◽  
Vol 16 (12) ◽  
pp. 2277-2286 ◽  
Author(s):  
Ye Wang ◽  
Jaap den Toonder ◽  
Ruth Cardinaels ◽  
Patrick Anderson
Keyword(s):  
Large Scale ◽  
Microfluidic Flow ◽  
Flow Generation ◽  
Artificial Cilia ◽  
Microfluidic Pumping

Magnetic artificial cilia capable of microfluidic flow generation fabricated using a novel roll pulling method with the potential for large-scale manufacturing.

scholarly journals Versatile microfluidic flow generated by moulded magnetic artificial cilia

2018 ◽  
Vol 263 ◽  
pp. 614-624 ◽  
Author(s):  
Shuaizhong Zhang ◽  
Ye Wang ◽  
Reinoud Lavrijsen ◽  
Patrick R. Onck ◽  
Jaap M.J. den Toonder
Keyword(s):  
Microfluidic Flow ◽  
Artificial Cilia

Open, Microfluidic Flow Cell for Studies of Interfacial Processes at Gas−Liquid Interfaces

2006 ◽  
Vol 78 (5) ◽  
pp. 1657-1664 ◽  
Author(s):  
Khanh C. Hoang ◽  
Dmitry Malakhov ◽  
William E. Momsen ◽  
Howard L. Brockman
Keyword(s):  
Flow Cell ◽  
Liquid Interfaces ◽  
Interfacial Processes ◽  
Microfluidic Flow

scholarly journals Biomechanics of Neutrophil Tethers

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 515
Author(s):  
Andrea Cugno ◽  
Alex Marki ◽  
Klaus Ley
Keyword(s):  
Cell Activation ◽  
Optical Trap ◽  
Biomechanical Properties ◽  
Force Microscopy ◽  
Advantages And Disadvantages ◽  
Atomic Force ◽  
Microfluidic Flow ◽  
Biomembrane Force Probe ◽  
Membrane Reservoir

Leukocytes, including neutrophils, which are propelled by blood flow, can roll on inflamed endothelium using transient bonds between selectins and their ligands, and integrins and their ligands. When such receptor–ligand bonds last long enough, the leukocyte microvilli become extended and eventually form thin, 20 m long tethers. Tether formation can be observed in blood vessels in vivo and in microfluidic flow chambers. Tethers can also be extracted using micropipette aspiration, biomembrane force probe, optical trap, or atomic force microscopy approaches. Here, we review the biomechanical properties of leukocyte tethers as gleaned from such measurements and discuss the advantages and disadvantages of each approach. We also review and discuss viscoelastic models that describe the dependence of tether formation on time, force, rate of loading, and cell activation. We close by emphasizing the need to combine experimental observations with quantitative models and computer simulations to understand how tether formation is affected by membrane tension, membrane reservoir, and interactions of the membrane with the cytoskeleton.

A droplet-based microfluidic flow cytometry enabling absolute quantification of single-cell proteins leveraging constriction channel

2021 ◽  
Vol 25 (4) ◽  
Author(s):  
Hongyu Yang ◽  
Yuanchen Wei ◽  
Beiyuan Fan ◽  
Lixing Liu ◽  
Ting Zhang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Absolute Quantification ◽  
Microfluidic Flow

scholarly journals Chemotactic Responses of Jurkat Cells in Microfluidic Flow-Free Gradient Chambers

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 384 ◽  
Author(s):  
Utku M. Sonmez ◽  
Adam Wood ◽  
Kyle Justus ◽  
Weijian Jiang ◽  
Fatima Syed-Picard ◽  
...  
Keyword(s):  
Immune Response ◽  
Cancer Progression ◽  
Soft Lithography ◽  
Cell Movement ◽  
Population Level ◽  
Jurkat Cells ◽  
Dependent Manner ◽  
Diverse Range ◽  
Microfluidic Flow ◽  
Cell Motion

Gradients of soluble molecules coordinate cellular communication in a diverse range of multicellular systems. Chemokine-driven chemotaxis is a key orchestrator of cell movement during organ development, immune response and cancer progression. Chemotaxis assays capable of examining cell responses to different chemokines in the context of various extracellular matrices will be crucial to characterize directed cell motion in conditions which mimic whole tissue conditions. Here, a microfluidic device which can generate different chemokine patterns in flow-free gradient chambers while controlling surface extracellular matrix (ECM) to study chemotaxis either at the population level or at the single cell level with high resolution imaging is presented. The device is produced by combining additive manufacturing (AM) and soft lithography. Generation of concentration gradients in the device were simulated and experimentally validated. Then, stable gradients were applied to modulate chemotaxis and chemokinetic response of Jurkat cells as a model for T lymphocyte motility. Live imaging of the gradient chambers allowed to track and quantify Jurkat cell migration patterns. Using this system, it has been found that the strength of the chemotactic response of Jurkat cells to CXCL12 gradient was reduced by increasing surface fibronectin in a dose-dependent manner. The chemotaxis of the Jurkat cells was also found to be governed not only by the CXCL12 gradient but also by the average CXCL12 concentration. Distinct migratory behaviors in response to chemokine gradients in different contexts may be physiologically relevant for shaping the host immune response and may serve to optimize the targeting and accumulation of immune cells to the inflammation site. Our approach demonstrates the feasibility of using a flow-free gradient chamber for evaluating cross-regulation of cell motility by multiple factors in different biologic processes.

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