Enhancement of inflection point focusing and rare-cell separations from untreated whole blood

Lab on a Chip ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 2861-2871 ◽  
Author(s):  
Dongwoo Lee ◽  
Yo-han Choi ◽  
Wonhee Lee
Keyword(s):  
High Throughput ◽  
Whole Blood ◽  
Lift Force ◽  
Inflection Point ◽  
Combined Effect ◽  
Inflection Points ◽  
Cell Separations

Inertial lift force towards inflection points is enhanced by the combined effect of U-shape channel and co-flow, which enables unprecedented high-throughput cell separations from untreated whole blood.

scholarly journals Resistance behaviour of carbon fibre-reinforced polymers subjected to lightning strikes: Experimental investigation and application

2019 ◽  
Vol 28 ◽  
pp. 2633366X1989227 ◽  
Author(s):  
Jian Chen ◽  
Zhengcai Fu ◽  
Yang Zhao
Keyword(s):  
Carbon Fibre ◽  
Surface Resistance ◽  
Inflection Point ◽  
Front Surface ◽  
Resistance Change ◽  
Reinforced Polymers ◽  
Change Rate ◽  
Lightning Strikes ◽  
Inflection Points ◽  
Carbon Fibre Reinforced Polymers

It is difficult to monitor lightning damage to carbon fibre-reinforced polymers (CFRPs) online. This work experimentally investigates the changes associated with the electrical resistance of CFRPs subjected to lightning strikes. Two kinds of simulated lightning currents with different amplitudes in the range of 10–80 kA were injected into the CFRP samples. By measuring and comparing the changes in the struck-side (front) surface resistance, the surface resistance of the side opposite to the struck-side (back) and the oblique resistance of each sample before and after the lightning strike, it was observed that inflection points exist in the curve of the resistance change rate. The resistance decreases with increasing peak currents before the inflection point and increases when the peak current goes beyond the inflection point. The change rate of the front surface resistance is more sensitive to the lightning damage than are those of the back surface resistance and the oblique resistance. Different simulated lightning currents have approximately the same action integrals at the inflection points of resistance change rate. The characteristics indicate that resistance change detection could be a possible method for the online monitoring of CFRP lightning damage.

scholarly journals High-throughput screening of large volumes of whole blood using structured illumination and fluorescent on-chip imaging

Lab on a Chip ◽  
2012 ◽  
Vol 12 (23) ◽  
pp. 4968 ◽  
Author(s):  
Serap Altay Arpali ◽  
Caglar Arpali ◽  
Ahmet F. Coskun ◽  
Hsin-Hao Chiang ◽  
Aydogan Ozcan
Keyword(s):  
High Throughput ◽  
Whole Blood ◽  
High Throughput Screening ◽  
Structured Illumination ◽  
On Chip

scholarly journals A GROWTH STUDY OF BEEF CATTLE

1981 ◽  
Vol 61 (4) ◽  
pp. 1041-1048 ◽  
Author(s):  
L. A. GOONEWARDENE ◽  
R. T. BERG ◽  
R. T. HARDIN
Keyword(s):  
Birth Weight ◽  
Beef Cattle ◽  
Inflection Point ◽  
Prediction Errors ◽  
Von Bertalanffy ◽  
University Of Alberta ◽  
Inflection Points ◽  
Richards Function ◽  
Residual Variances ◽  
The University

Four growth equations (Richards, Brody, Von Bertalanffy and Logistic) were fitted to data from two breed groups of female beef cattle, purebred Hereford (HE) and synthetic of Charolais, Angus and Galloway (SY), which were maintained at the University of Alberta beef breeding research ranch at Kinsella, Alberta. All functions fitted three common parameters (an asymptote, an integration constant and a maturing rate parameter). Two functions (Von Bertalanffy and Logistic) had fixed inflection points while the Richards function fitted the inflection point as a fourth parameter. The Brody function had no inflection point. Based on the R2, mean prediction errors (MPE) and residual variances, the Richards function, with a variable inflection point, provided the best overall and the most consistent fit to both sets of data, followed by the Brody function with no inflection point. The Richards was the only function that could predict birth weight with any degree of accuracy. The Logistic and Von Bertalanffy functions, with fixed inflection points, provided poor estimates of actual weights at each end of the growth curve, overestimating birth weight and converging too early leading to underestimation of adult weight or the asymptote. Intermediate weights were reasonably well estimated by these functions. The HE group showed a 28% greater maturing rate compared with the SY based on the Richards function.

scholarly journals A rapid high-throughput sequencing-based approach for the identification of unknown bacterial pathogens in whole blood

2020 ◽  
Vol 6 (6) ◽  
pp. FSO476
Author(s):  
Ofir Israeli ◽  
Efi Makdasi ◽  
Inbar Cohen-Gihon ◽  
Anat Zvi ◽  
Shirley Lazar ◽  
...  
Keyword(s):  
High Throughput ◽  
Whole Blood ◽  
High Throughput Sequencing ◽  
High Background ◽  
Blood Samples ◽  
Bacterial Enrichment ◽  
Host Pathogen ◽  
High Throughput Dna Sequencing ◽  
Human Blood Samples ◽  
Pathogen Dna

High-throughput DNA sequencing (HTS) of pathogens in whole blood samples is hampered by the high host/pathogen nucleic acids ratio. We describe a novel and rapid bacterial enrichment procedure whose implementation is exemplified in simulated bacteremic human blood samples. The procedure involves depletion of the host DNA, rapid HTS and bioinformatic analyses. Following this procedure, Y. pestis, F. tularensis and B. anthracis spiked-in samples displayed an improved host/pathogen DNA ratio of 2.5–5.9 orders of magnitude, in samples with bacteria spiked-in at 103–105 CFU/ml. The procedure described in this study enables rapid and detailed metagenomic profiling of pathogens within 8–9 h, circumventing the challenges imposed by the high background present in the bacteremic blood and by the unknown nature of the sample.

Microfluidic Pipette Tip for High-Purity and High-Throughput Blood Plasma Separation from Whole Blood

2017 ◽  
Vol 89 (3) ◽  
pp. 1439-1444 ◽  
Author(s):  
Byeongyeon Kim ◽  
Sein Oh ◽  
Dongwon You ◽  
Sungyoung Choi
Keyword(s):  
Blood Plasma ◽  
High Throughput ◽  
High Purity ◽  
Whole Blood ◽  
Plasma Separation ◽  
Pipette Tip ◽  
Blood Plasma Separation

A Microchannel With Repeated Sharp Corners for Single Stream Particle Focusing

2014 ◽  
Author(s):  
Liangliang Fan ◽  
Xukun He ◽  
Liang Zhao ◽  
Yu Han ◽  
Jiang Zhe
Keyword(s):  
Centrifugal Force ◽  
High Throughput ◽  
Flow Rate ◽  
Lift Force ◽  
Channel Wall ◽  
Sharp Corner ◽  
Force Effect ◽  
Particle Focusing ◽  
Wide Range ◽  
Sharp Corners

A new microfluidic device for fast and high throughput microparticle focusing is reported. The particle focusing is based on the combination of inertial lift force effect and centrifugal force effect generated in a microchannel with a series of repeated sharp corners on one side of the channel wall. The inertial lift force effect induces two focused particles streams in the microchannel, and the centrifugal force generated at the sharp corner structures tends to drive the particles laterally away from the corner. With the use of a series of the repeated, sharp corner structures, a single and highly focused particle stream was achieved near the straight channel wall at a wide range of flow rates. In comparison to other hydrodynamic particle focusing methods, this method is less sensitive to the flow rate and can work at a higher flow rate (high throughput). With its simple structure and operation, and high throughput, this method can be potentially used in microparticle focusing processes in a variety of lab-on-a chip applications.

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