Cell elasticity measurement using a microfluidic device with real-time pressure feedback

Lab on a Chip ◽  
2020 ◽  
Vol 20 (13) ◽  
pp. 2343-2353 ◽  
Author(s):  
Zhenlin Chen ◽  
Yonggang Zhu ◽  
Dong Xu ◽  
Md. Mahbub Alam ◽  
Lingling Shui ◽  
...  
Keyword(s):  
Real Time ◽  
Microfluidic Device ◽  
Cell Biology ◽  
Time Pressure ◽  
Disease Diagnosis ◽  
Mechanical State ◽  
Pressure Feedback ◽  
State Variation ◽  
Cell Elasticity ◽  
Elasticity Measurement

The study of cell elasticity provides new insights into not only cell biology but also disease diagnosis based on cell mechanical state variation.

scholarly journals A Microfluidic Micropipette Aspiration Device to Study Single-Cell Mechanics Inspired by the Principle of Wheatstone Bridge

Micromachines ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 131 ◽  
Author(s):  
Yong-Jiang Li ◽  
Yu-Nong Yang ◽  
Hai-Jun Zhang ◽  
Chun-Dong Xue ◽  
De-Pei Zeng ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Hela Cells ◽  
Cell Biology ◽  
Flow Direction ◽  
Single Cells ◽  
Wheatstone Bridge ◽  
Biomechanical Properties ◽  
Disease Diagnosis ◽  
Micropipette Aspiration

The biomechanical properties of single cells show great potential for early disease diagnosis and effective treatments. In this study, a microfluidic device was developed for quantifying the mechanical properties of a single cell. Micropipette aspiration was integrated into a microfluidic device that mimics a classical Wheatstone bridge circuit. This technique allows us not only to effectively alter the flow direction for single-cell trapping, but also to precisely control the pressure exerted on the aspirated cells, analogous to the feature of the Wheatstone bridge that can precisely control bridge voltage and current. By combining the micropipette aspiration technique into the microfluidic device, we can effectively trap the microparticles and Hela cells as well as measure the deformability of cells. The Young’s modulus of Hela cells was evaluated to be 387 ± 77 Pa, which is consistent with previous micropipette aspiration studies. The simplicity, precision, and usability of our device show good potential for biomechanical trials in clinical diagnosis and cell biology research.

101-LB: Real-Time Monitoring of Cholesterol Levels Using a Microfluidic Device

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 101-LB
Author(s):  
ABHINAV BHUSHAN ◽  
SONALI J. KARNIK
Keyword(s):  
Real Time ◽  
Microfluidic Device ◽  
Real Time Monitoring ◽  
Cholesterol Levels

A nanostructured microfluidic device for plasmonic-assisted electrochemical detection of hydrogen peroxide released from cancer cells

Nanoscale ◽  
2021 ◽  
Author(s):  
Carolina del Real Mata ◽  
Roozbeh Siavash Moakhar ◽  
Sayed Iman Isaac Hosseini ◽  
Mahsa Jalali ◽  
Sara Mahshid
Keyword(s):  
Hydrogen Peroxide ◽  
Cancer Cells ◽  
Real Time ◽  
Electrochemical Detection ◽  
Microfluidic Device ◽  
Cancer Biomarker ◽  
Cancer Diagnostics ◽  
Liquid Biopsies ◽  
Non Invasive

Non-invasive liquid biopsies offer hope for a rapid, risk-free, real-time glimpse into cancer diagnostics. Recently, hydrogen peroxide (H2O2) is identified as a cancer biomarker due to continued release from cancer...

Using Real-Time Pressure Data for Well Placement Planning

2006 ◽  
Author(s):  
Mike Parker ◽  
Robert N. Bradford ◽  
Laurence Ward Corbett ◽  
Robin Noel Heim ◽  
Christina Leigh Isakson ◽  
...  
Keyword(s):  
Real Time ◽  
Time Pressure ◽  
Pressure Data ◽  
Well Placement

Real-Time Detection of Escherichia coli in Water Pipe Using a Microfluidic Device with One-Step Latex Immunoagglutination Assay

2009 ◽  
Vol 52 (3) ◽  
pp. 1031-1039
Author(s):  
J.-Y. Yoon ◽  
J.-H. Han ◽  
C. Y. Choi ◽  
M. Bui ◽  
R. G. Sinclair
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Microfluidic Device ◽  
Water Pipe ◽  
One Step ◽  
Real Time Detection

scholarly journals Guided Axon Outgrowth of Neurons by Real-Time Femtosecond Laser Penetration on a 4 μm Thick Thin-Glass Sheet

2021 ◽  
Author(s):  
Dian Anggraini ◽  
Xun Liu ◽  
Kazunori Okano ◽  
Yo Tanaka ◽  
Naoyuki Inagaki ◽  
...  
Keyword(s):  
Real Time ◽  
Microfluidic Device ◽  
Glass Sheet ◽  
Axon Outgrowth ◽  
Neuronal Regeneration ◽  
Thin Glass ◽  
Manipulation System ◽  
Micro Holes ◽  
Fs Laser ◽  
Guidance Molecule

Transplantation of scaffold-embedded guided neurons has been reported to increase neuronal regeneration following brain injury. However, precise axonal integration between host and transplant neurons to form functional synapses remains a major problem. This study aims to develop a real-time femtosecond (fs) laser penetration on a 4 μm thick thin-glass sheet to promote guided axon outgrowth influenced by molecular gradients in a microfluidic device. The device enables the introduction of the guidance molecule (i.e., netrin-1), neuronal culture, and manipulation by fs laser. After fabricating multiple micro-holes on the thin-glass sheet using fs laser, netrin-1 gradients with radial concentrations are generated in the chamber, affecting axon outgrowth and guidance. A majority of axons (~92%) experiences guided outgrowth with positive angular changes towards netrin-1 gradients. These results demonstrate the capability of the precise and real-time manipulation system based on a fs laser and a microfluidic device to control the growth of neurons.

Sign in / Sign up

Export Citation Format

Share Document