scholarly journals The effect of switchgrass plant cell wall properties on its deconstruction by thermochemical pretreatments coupled with fungal enzymatic hydrolysis or Clostridium thermocellum consolidated bioprocessing

2020 ◽  
Vol 22 (22) ◽  
pp. 7924-7945
Author(s):  
Ninad Kothari ◽  
Samarthya Bhagia ◽  
Yunqiao Pu ◽  
Chang Geun Yoo ◽  
Mi Li ◽  
...  
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Plant Cell ◽  
Clostridium Thermocellum ◽  
Plant Cell Wall ◽  
Consolidated Bioprocessing ◽  
Wall Properties ◽  
Cell Wall Properties

Switchgrass, thermochemically pretreated switchgrass, and corresponding biologically digested residues were characterized to understand the process of lignocelluose deconstruction.

Evaluation of plant cell wall degrading enzyme production by Clostridium thermocellum B8 in the presence of raw agricultural wastes

2015 ◽  
Vol 105 ◽  
pp. 97-105 ◽  
Author(s):  
Pedro Ricardo V. Hamann ◽  
Dayane L. Serpa ◽  
Amanda Souza Barreto da Cunha ◽  
Brenda R. de Camargo ◽  
Karen Ofuji Osiro ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Clostridium Thermocellum ◽  
Enzyme Production ◽  
Plant Cell Wall ◽  
Agricultural Wastes ◽  
Degrading Enzyme ◽  
Cell Wall Degrading Enzyme

Mannan specific family 35 carbohydrate-binding module (CtCBM35) of Clostridium thermocellum: structure analysis and ligand binding

Biologia ◽  
2014 ◽  
Vol 69 (10) ◽  
Author(s):  
Arabinda Ghosh ◽  
Anil Verma ◽  
Ana Luis ◽  
Joana Bras ◽  
Carlos Fontes ◽  
...  
Keyword(s):  
Cell Wall ◽  
Ligand Binding ◽  
Binding Affinity ◽  
Plant Cell ◽  
Clostridium Thermocellum ◽  
Plant Cell Wall ◽  
Konjac Glucomannan ◽  
Carbohydrate Binding ◽  
Carbohydrate Binding Module ◽  
Affinity Electrophoresis

AbstractThe three-dimensional model of the CtCBM35 (Cthe 2811), i.e. the family 35 carbohydrate binding module (CBM) from the Clostridium thermocellum family 26 glycoside hydrolase (GH) β-mannanase, generated by Modeller9v8 displayed predominance of β-sheets arranged as β-sandwich fold. Multiple sequence alignment of CtCBM35 with other CBM35s showed a conserved signature sequence motif Trp-Gly-Tyr, which is probably a specific determinant for mannan binding. Cloned CtCBM35 from Clostridium thermocellum ATCC 27405 was a homogenous, soluble 16 kDa protein. Ligand binding analysis of CtCBM35 by affinity electrophoresis displayed higher binding affinity against konjac glucomannan (K a = 2.5 × 105 M−1) than carob galactomannan (K a = 1.4 × 105 M−1). The presence of Ca2+ ions imparted slightly higher binding affinity of CtCBM35 against carob galactomannan and konjac glucomannan than without Ca2+ ion additive. However, CtCBM35 exhibited a low ligand-binding affinity K a = 2.5 × 10−5 M−1 with insoluble ivory nut mannan. Ligand binding study by fluorescence spectroscopy showed K a against konjac glucomannan and carob galactomannan, 2.4 × 105 M−1 and 1.44 × 105 M−1, and ΔG of binding −27.0 and −25.0 kJ/mol, respectively, substantiating the findings of affinity electrophoresis. Ca2+ ions escalated the thermostability of CtCBM35 and its melting temperature was shifted to 70°C from initial 55°C. Therefore thermostable CtCBM35 targets more β-(1,4)-manno-configured ligands from plant cell wall hemicellulosic reservoir. Thus a non-catalytic CtCBM35 of multienzyme cellulosomal enzymes may gain interest in the biofuel and food industry in the form of released sugars by targeting plant cell wall polysaccharides.

scholarly journals The Mechanisms of Plant Cell Wall Deconstruction during Enzymatic Hydrolysis

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e108313 ◽  
Author(s):  
Lisbeth G. Thygesen ◽  
Emil E. Thybring ◽  
Katja S. Johansen ◽  
Claus Felby
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Plant Cell ◽  
Plant Cell Wall

The role of ester groups in resistance of plant cell wall polysaccharides to enzymatic hydrolysis

1989 ◽  
Vol 20-21 (1) ◽  
pp. 45-61 ◽  
Author(s):  
K. Grohmann ◽  
D. J. Mitchell ◽  
M. E. Himmel ◽  
B. E. Dale ◽  
H. A. Schroeder
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Polysaccharides

Structural elucidation of whole lignin from Eucalyptus based on preswelling and enzymatic hydrolysis

2015 ◽  
Vol 17 (3) ◽  
pp. 1589-1596 ◽  
Author(s):  
Jia-Long Wen ◽  
Shao-Long Sun ◽  
Tong-Qi Yuan ◽  
Run-Cang Sun
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Structural Elucidation ◽  
Molecular Characteristics ◽  
Green Chemicals

The structural elucidation of whole lignin in the plant cell wall is extremely important for providing a representative lignin to understand the molecular characteristics of lignin in plants, and develop lignin-based polymers and green chemicals under the current biorefinery scenario.

scholarly journals Cell-wall properties contributing to improved deconstruction by alkaline pre-treatment and enzymatic hydrolysis in diverse maize (Zea maysL.) lines

2015 ◽  
Vol 66 (14) ◽  
pp. 4305-4315 ◽  
Author(s):  
Muyang Li ◽  
Marlies Heckwolf ◽  
Jacob D. Crowe ◽  
Daniel L. Williams ◽  
Timothy D. Magee ◽  
...  
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Wall Properties ◽  
Pre Treatment ◽  
Cell Wall Properties

scholarly journals Heterologous Expression of Plant Cell Wall Degrading Enzymes for Effective Production of Cellulosic Biofuels

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Sang-Kyu Jung ◽  
Vinuselvi Parisutham ◽  
Seong Hun Jeong ◽  
Sung Kuk Lee
Keyword(s):  
Cell Wall ◽  
Heterologous Expression ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Plant Biomass ◽  
Consolidated Bioprocessing ◽  
Host Organism ◽  
Cell Wall Degrading Enzymes ◽  
Degrading Enzymes ◽  
The Cost

A major technical challenge in the cost-effective production of cellulosic biofuel is the need to lower the cost of plant cell wall degrading enzymes (PCDE), which is required for the production of sugars from biomass. Several competitive, low-cost technologies have been developed to produce PCDE in different host organisms such asEscherichia coli, Zymomonas mobilis, and plant. Selection of an ideal host organism is very important, because each host organism has its own unique features. Synthetic biology-aided tools enable heterologous expression of PCDE in recombinantE. coliorZ. mobilisand allow successful consolidated bioprocessing (CBP) in these microorganisms.In-plantaexpression provides an opportunity to simplify the process of enzyme production and plant biomass processing and leads to self-deconstruction of plant cell walls. Although the future of currently available technologies is difficult to predict, a complete and viable platform will most likely be available through the integration of the existing approaches with the development of breakthrough technologies.

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