scholarly journals Principal component analysis facilitated fast and noninvasive Raman spectroscopic imaging of plant cell wall pectin distribution and interaction with enzymatic hydrolysis

2020 ◽  
Vol 51 (12) ◽  
pp. 2458-2467
Author(s):  
Qing He ◽  
Olga A. Zabotina ◽  
Chenxu Yu
Keyword(s):  
Principal Component Analysis ◽  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Spectroscopic Imaging ◽  
Principal Component ◽  
Component Analysis ◽  
Raman Spectroscopic

scholarly journals The Mechanisms of Plant Cell Wall Deconstruction during Enzymatic Hydrolysis

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e108313 ◽  
Author(s):  
Lisbeth G. Thygesen ◽  
Emil E. Thybring ◽  
Katja S. Johansen ◽  
Claus Felby
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Plant Cell ◽  
Plant Cell Wall

scholarly journals The effect of switchgrass plant cell wall properties on its deconstruction by thermochemical pretreatments coupled with fungal enzymatic hydrolysis or Clostridium thermocellum consolidated bioprocessing

2020 ◽  
Vol 22 (22) ◽  
pp. 7924-7945
Author(s):  
Ninad Kothari ◽  
Samarthya Bhagia ◽  
Yunqiao Pu ◽  
Chang Geun Yoo ◽  
Mi Li ◽  
...  
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Plant Cell ◽  
Clostridium Thermocellum ◽  
Plant Cell Wall ◽  
Consolidated Bioprocessing ◽  
Wall Properties ◽  
Cell Wall Properties

Switchgrass, thermochemically pretreated switchgrass, and corresponding biologically digested residues were characterized to understand the process of lignocelluose deconstruction.

scholarly journals Structural Polymorphism of Chitin and Chitosan in Fungal Cell Walls From Solid-State NMR and Principal Component Analysis

2021 ◽  
Vol 8 ◽  
Author(s):  
Liyanage D. Fernando ◽  
Malitha C. Dickwella Widanage ◽  
Jackson Penfield ◽  
Andrew S. Lipton ◽  
Nancy Washton ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Cell Wall ◽  
Cell Walls ◽  
Chemical Shifts ◽  
Principal Component ◽  
Component Analysis ◽  
Antifungal Drugs ◽  
Fungal Cell ◽  
Structural Resistance ◽  
Chitin And Chitosan

Chitin is a major carbohydrate component of the fungal cell wall and a promising target for novel antifungal agents. However, it is technically challenging to characterize the structure of this polymer in native cell walls. Here, we recorded and compared 13C chemical shifts of chitin using isotopically enriched cells of six Aspergillus, Rhizopus, and Candida strains, with data interpretation assisted by principal component analysis (PCA) and linear discriminant analysis (LDA) methods. The structure of chitin is found to be intrinsically heterogeneous, with peak multiplicity detected in each sample and distinct fingerprints observed across fungal species. Fungal chitin exhibits partial similarity to the model structures of α- and γ-allomorphs; therefore, chitin structure is not significantly affected by interactions with other cell wall components. Addition of antifungal drugs and salts did not significantly perturb the chemical shifts, revealing the structural resistance of chitin to external stress. In addition, the structure of the deacetylated form, chitosan, was found to resemble a relaxed two-fold helix conformation. This study provides high-resolution information on the structure of chitin and chitosan in their cellular contexts. The method is applicable to the analysis of other complex carbohydrates and polymer composites.

The role of ester groups in resistance of plant cell wall polysaccharides to enzymatic hydrolysis

1989 ◽  
Vol 20-21 (1) ◽  
pp. 45-61 ◽  
Author(s):  
K. Grohmann ◽  
D. J. Mitchell ◽  
M. E. Himmel ◽  
B. E. Dale ◽  
H. A. Schroeder
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Polysaccharides
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