Auricularia polytricha ethanol crude extract from sequential maceration induces lipid accumulation and inflammatory suppression in RAW264.7 macrophages

2021 ◽  
Author(s):  
Naunpun Sangphech ◽  
Chanin Sillapachaiyaporn ◽  
Sunita Nilkhet ◽  
Siriporn Chuchawankul
Keyword(s):  
Crude Extract ◽  
Lipid Accumulation ◽  
In Silico ◽  
Bioactive Compound ◽  
Auricularia Polytricha ◽  
Raw264.7 Macrophages ◽  
Anti Inflammation

Using LC-MS combine with in silico approch, we predict that 15d-PGJ2-G is a bioactive compound in Auricularia polytricha ethanol crude interacted with PPARγ to regulate lipid accumulation and anti-inflammation in RAW264.7 macrophages.

scholarly journals Upregulation of miR-150-5p alleviates LPS-induced inflammatory response and apoptosis of RAW264.7 macrophages by targeting Notch1

2020 ◽  
Vol 15 (1) ◽  
pp. 544-552
Author(s):  
Xiaoyan Deng ◽  
Zhixing Lin ◽  
Chao Zuo ◽  
Yanjie Fu
Keyword(s):  
Inflammatory Response ◽  
Underlying Mechanism ◽  
Notch Receptor ◽  
Raw264.7 Cells ◽  
Luciferase Reporter ◽  
Raw264.7 Macrophages ◽  
Factor Α ◽  
Anti Inflammation ◽  
Necrosis Factor ◽  
Dual Luciferase Reporter Assay

AbstractCirculating miR-150-5p has been identified as a prognostic marker in patients with critical illness and sepsis. Herein, we aimed to further explore the role and underlying mechanism of miR-150-5p in sepsis. Quantitative real-time-PCR assay was performed to detect the expression of miR-150-5p upon stimulation with lipopolysaccharide (LPS) in RAW264.7 cells. The levels of tumor necrosis factor-α, interleukin (IL)-6 and IL-1β were measured by ELISA assay. Cell apoptosis was determined using flow cytometry. Western blot was used to assess notch receptor 1 (Notch1) expression in LPS-induced RAW264.7 cells. Dual-luciferase reporter assay was employed to validate the target of miR-150-5p. Our data showed that miR-150-5p was downregulated and Notch1 was upregulated in LPS-stimulated RAW264.7 cells. miR-150-5p overexpression or Notch1 silencing alleviated LPS-induced inflammatory response and apoptosis in RAW264.7 cells. Moreover, Notch1 was a direct target of miR-150-5p. Notch1 abated miR-150-5p-mediated anti-inflammation and anti-apoptosis in LPS-induced RAW264.7 cells. miR-150-5p alleviated LPS-induced inflammatory response and apoptosis at least partly by targeting Notch1 in RAW264.7 cells, highlighting miR-150-5p as a target in the development of anti-inflammation and anti-apoptosis drugs for sepsis treatment.

GR-mediated anti-inflammation of α-boswellic acid: Insights from in vitro and in silico studies

2021 ◽  
Vol 155 ◽  
pp. 112379
Author(s):  
Jie Zhang ◽  
Jiarui Zhao ◽  
Yantong Sun ◽  
Yuan Liang ◽  
Jingqi Zhao ◽  
...  
Keyword(s):  
In Silico ◽  
Boswellic Acid ◽  
In Silico Studies ◽  
Anti Inflammation

scholarly journals TOXICITY TEST OF EXTRACTS OF THE FOUR SEA CUCUMBER (FAMILY HOLOTHURIIDAE) FROM EAST PENJALIRAN ISLAND, SERIBU ISLANDS, JAKARTA BASED ON THE BRINE SHRIMP LETHALITY TEST (BSLT)

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Arum Albuntana ◽  
Yasman Yasman ◽  
Wisnu Wardhana
Keyword(s):  
Crude Extract ◽  
Brine Shrimp ◽  
National Park ◽  
Sea Cucumber ◽  
Bioactive Compound ◽  
Active Species ◽  
Brine Shrimp Lethality ◽  
Water Fraction ◽  
Lc50 Value ◽  
Toxicity Level

<p>Sea cucumber is one of the marine bioactive compounds resources . The properties of the bioactive compound are biologically shown by their functions as anticancer, antifungi, hemolytic, and immunomodulatoryt. The purpose of this research is to predict (determine) the toxicity level of the extracts of Actinopyga miliaris, Holothuria leucospilota, Bohadschia argus, and Bohadschia marmorata collected from Penjaliran Timur Island, Seribu Island National Park Jakarta. The method used in this research is Brine Shrimp Lethality Test (BSLT). The result of this research showed that all of the extract from those species are active in BSLT, indicated by LC50 values of less than 1000 μg/ml. Bohadcshia argus is highest active species indicated by LC50 value 69,254 μg/ml. Water fraction of H. leucospilota’s crude extract is the most active fraction indicated by LC50 50,968 μg/ml.</p><p>Keywords: Brine Shrimp Lethality Test, Crude Extract, Fractionation, Family Holothuriidae, Sea Cucumber</p>

Bioactive potential of culturable fungal endophytes isolated from leaf of Catharanthus roseus (L.) G. Don

2021 ◽  
Vol 21 ◽  
Author(s):  
Sucheta Singh ◽  
Surjeet Verma ◽  
Dharmendra Kumar Yadav ◽  
Anant Kumar ◽  
Rekha Tyagi ◽  
...  
Keyword(s):  
Catharanthus Roseus ◽  
Crude Extract ◽  
Inhibitory Concentration ◽  
Efflux Pump ◽  
Fungal Endophytes ◽  
Bacterial Pathogen ◽  
Bioactive Compound ◽  
Major Constituent ◽  
Anti Inflammatory ◽  
Bioactive Potential

Introduction: Endophyte is considered as a source of natural bioactive secondary metabolites that provides an array of bioactive lead compounds. The present study was aimed to determine the antimicrobial and anti-inflammatory potential of fungal endophytes isolated from Catharanthus roseus. Methods: A total of seven fungal endophytes crude extract were screened against bacterial pathogens. Of these, Curvularia geniculata CATDLF7 crude extract exhibited the most potent inhibitory activity against bacterial pathogen. Hence, CATDLF7 crude extract was subjected to the chromatographic separation. This purification leads to the isolation of six pure compounds (1PS - 6PS). Of these, 3PS was found to be a major constituent and most effective against clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) with minimum inhibitory concentration (MIC) values ranging from 100 to 200 μg/ml. Based on the spectroscopic data, 3PS was characterized as α,β-dehydrocurvularin. This compound also showed synergistic interaction with norfloxacin, and reduced its MIC up to 32-folds with fractional inhibitory concentration index (FICI) of 0.09. Results: To understand the possible antibacterial mechanism of action, α,β-dehydrocurvularin alone (100 μg/ml) exhibited efflux pump inhibitory potential by 0.84 fold decreasing in ethidium bromide (EtBr) fluorescence. In addition, α,β-dehydrocurvularin inhibited inflammatory cytokines TNF-α and IL-6 production which is further validated by molecular docking score -4.921 and -5.641 respectively for understanding orientation and binding affinity. Conclusion: Overall the results highlighted to identifying bioactive compound α,β-dehydrocurvularin which could be used as an antimicrobial and anti-inflammatory agents.

Pomegranate peel polyphenols inhibit lipid accumulation and enhance cholesterol efflux in raw264.7 macrophages

2016 ◽  
Vol 7 (7) ◽  
pp. 3201-3210 ◽  
Author(s):  
Shengjuan Zhao ◽  
Jianke Li ◽  
Lifang Wang ◽  
Xiaoxia Wu
Keyword(s):  
Lipid Accumulation ◽  
Cholesterol Efflux ◽  
Foam Cell ◽  
Cell Formation ◽  
Foam Cell Formation ◽  
Pomegranate Peel ◽  
Raw264.7 Macrophages

Pomegranate peel polyphenols hindered ox-LDL-induced raw264.7 foam cell formation, by decreasing CD36 and promoting ABCA1 and LXRα expression.

scholarly journals Quercetin Suppresses the Progression of Atherosclerosis by Regulating MST1-Mediated Autophagy in ox-LDL-Induced RAW264.7 Macrophage Foam Cells

2019 ◽  
Vol 20 (23) ◽  
pp. 6093 ◽  
Author(s):  
Hui Cao ◽  
Qingling Jia ◽  
Li Yan ◽  
Chuan Chen ◽  
Sanli Xing ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Foam Cell ◽  
Cell Model ◽  
Foam Cells ◽  
Foam Cell Formation ◽  
Raw264.7 Macrophages ◽  
Age Related ◽  
Raw264.7 Macrophage ◽  
Senescence Phenotype

Objective: To investigate the process by which quercetin suppresses atherosclerosis by upregulating MST1-mediated autophagy in RAW264.7 macrophages. Methods: An in vitro foam cell model was established by culturing RAW264.7 macrophages with oxidized low-density lipoprotein (ox-LDL). The cells were treated with quercetin alone or in combination with the autophagy inhibitor, 3-methyladenine, and autophagy agonist, rapamycin. Cell viability was detected with a CCK-8 kit. Lipid accumulation was detected by oil red O staining, senescence was detected by SA-β-gal (senescence-associated β-galactosidase) staining, reactive oxygen species were detected by ROS assay kit. Autophagosomes and mitochondria were detected by transmission electron microscope (TEM), and expression of MST1, LC3-II/I, Beclin1, Bcl-2, P21, and P16 were detected by immunofluorescence and Western blot. Results: Ox-LDL induced RAW264.7 macrophage-derived foam cell formation, reduced survival, aggravated cell lipid accumulation, and induced a senescence phenotype. This was accompanied by decreased formation of autophagosome; increased expression of P53, P21, and P16; and decreased expression of LC3-II/I and Beclin1. After intervention with quercetin, the cell survival rate was increased, and lipid accumulation and senescence phenotype were reduced. Furthermore, the expression of LC3-II/I and Beclin1 were increased, which was consistent with the ability of quercetin to promote autophagy. Ox-LDL also increased the expression of MST1, and this increase was blocked by quercetin, which provided a potential mechanism by which quercetin may protect foam cells against age-related detrimental effects. Conclusion: Quercetin can inhibit the formation of foam cells induced by ox-LDL and delay senescence. The mechanism may be related to the regulation of MST1-mediated autophagy of RAW264.7 cells.

scholarly journals Momordica charantia Suppresses Inflammation and Glycolysis in Lipopolysaccharide-Activated RAW264.7 Macrophages

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3783
Author(s):  
Shi Yan Lee ◽  
Won Fen Wong ◽  
Jiyang Dong ◽  
Kian-Kai Cheng
Keyword(s):  
Glucose Metabolism ◽  
Inflammatory Response ◽  
Macrophage Activation ◽  
Nuclear Translocation ◽  
Lactate Production ◽  
Bioactive Compound ◽  
Momordica Charantia ◽  
Activated Macrophages ◽  
Raw264.7 Macrophages ◽  
Tnf Α

Macrophage activation is a key event that triggers inflammatory response. The activation is accompanied by metabolic shift such as upregulated glucose metabolism. There are accumulating evidences showing the anti-inflammatory activity of Momordica charantia. However, the effects of M. charantia on inflammatory response and glucose metabolism in activated macrophages have not been fully established. The present study aimed to examine the effect of M. charantia in modulating lipopolysaccharide (LPS)-induced inflammation and perturbed glucose metabolism in RAW264.7 murine macrophages. The results showed that LPS-induced NF-κB (p65) nuclear translocation was inhibited by M. charantia treatment. In addition, M. charantia was found to reduce the expression of inflammatory genes including IL6, TNF-α, IL1β, COX2, iNOS, and IL10 in LPS-treated macrophages. Furthermore, the data showed that M. charantia reduced the expression of GLUT1 and HK2 genes and lactate production (−28%), resulting in suppression of glycolysis. Notably, its effect on GLUT1 gene expression was found to be independent of LPS-induced inflammation. A further experiment also indicated that the bioactivities of M. charantia may be attributed to its key bioactive compound, charantin. Taken together, the study provided supporting evidences showing the potential of M. charantia for the treatment of inflammatory disorders.

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