scholarly journals Iron Fenton oxidation of 2′-deoxyguanosine in physiological bicarbonate buffer yields products consistent with the reactive oxygen species carbonate radical anion not the hydroxyl radical

2020 ◽  
Vol 56 (68) ◽  
pp. 9779-9782 ◽  
Author(s):  
Aaron M. Fleming ◽  
Cynthia J. Burrows
Keyword(s):  
Reactive Oxygen Species ◽  
Hydroxyl Radical ◽  
Fenton Reaction ◽  
Radical Anion ◽  
Reactive Oxygen ◽  
Fenton Oxidation ◽  
Oxygen Species ◽  
Bicarbonate Buffer ◽  
Carbonate Radical ◽  
Carbonate Radical Anion

Fe(ii)-Fenton reaction in bicarbonate buffer yields CO3˙−, not HO˙, oxidizing 2′-deoxyguanosine to yield 8-oxo-7,8-dihydro-2′-deoxyguanosine with no ribose damage.

scholarly journals On the irrelevancy of hydroxyl radical to DNA damage from oxidative stress and implications for epigenetics

2020 ◽  
Vol 49 (18) ◽  
pp. 6524-6528 ◽  
Author(s):  
Aaron M. Fleming ◽  
Cynthia J. Burrows
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Dna Damage ◽  
Hydroxyl Radical ◽  
Radical Anion ◽  
Oxidation Products ◽  
Oxygen Species ◽  
Carbonate Radical ◽  
Guanine Oxidation ◽  
Carbonate Radical Anion

Carbonate radical anion, not hydroxyl radical, is the principal reactive oxygen species generated from endogenous oxidative stress endowing epigenetic features to guanine oxidation products in DNA.

A Novel pH-responsive Fe-MOF System for Enhanced Cancer Treatment Mediated by Fenton Reaction

2021 ◽  
Author(s):  
Senlin Wang ◽  
Hong-Shuai Wu ◽  
Kai Sun ◽  
Jinzhong Hu ◽  
Fanghui Chen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydroxyl Radical ◽  
Cancer Treatment ◽  
Cell Apoptosis ◽  
Fenton Reaction ◽  
Reactive Oxygen ◽  
Cationic Polymer ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Ph Responsive

Recently, the toxic hydroxyl radical (·OH) has received wide interest in inducing cell apoptosis by increasing the intracellular reactive oxygen species (ROS) levels. Herein, a cationic polymer (MV-PAH) was rationally...

scholarly journals Reactive Oxygen Species Contribute to the Bactericidal Effects of the Fluoroquinolone Moxifloxacin in Streptococcus pneumoniae

2015 ◽  
Vol 60 (1) ◽  
pp. 409-417 ◽  
Author(s):  
M. J. Ferrándiz ◽  
A. J. Martín-Galiano ◽  
C. Arnanz ◽  
T. Zimmerman ◽  
A. G. de la Campa
Keyword(s):  
Reactive Oxygen Species ◽  
Streptococcus Pneumoniae ◽  
Hydroxyl Radical ◽  
Fenton Reaction ◽  
Reactive Oxygen ◽  
Fold Increase ◽  
Oxygen Species ◽  
Acetyl Coa ◽  
Transcriptomic Response ◽  
Content Type

ABSTRACTWe studied the transcriptomic response ofStreptococcus pneumoniaeto the fluoroquinolone moxifloxacin at a concentration that inhibits DNA gyrase. Treatment of the wild-type strain R6, at a concentration of 10× the MIC, triggered a response involving 132 genes after 30 min of treatment. Genes from several metabolic pathways involved in the production of pyruvate were upregulated. These included 3 glycolytic enzymes, which ultimately convert fructose 6-phosphate to pyruvate, and 2 enzymes that funnel phosphate sugars into the glycolytic pathway. In addition, acetyl coenzyme A (acetyl-CoA) carboxylase was downregulated, likely leading to an increase in acetyl-CoA. When coupled with an upregulation in formate acetyltransferase, an increase in acetyl-CoA would raise the production of pyruvate. Since pyruvate is converted by pyruvate oxidase (SpxB) into hydrogen peroxide (H2O2), an increase in pyruvate would augment intracellular H2O2. Here, we confirm a 21-fold increase in the production of H2O2and a 55-fold increase in the amount of hydroxyl radical in cultures treated during 4 h with moxifloxacin. This increase in hydroxyl radical through the Fenton reaction would damage DNA, lipids, and proteins. These reactive oxygen species contributed to the lethality of the drug, a conclusion supported by the observed protective effects of an SpxB deletion. These results support the model whereby fluoroquinolones cause redox alterations. The transcriptional response ofS. pneumoniaeto moxifloxacin is compared with the response to levofloxacin, an inhibitor of topoisomerase IV. Levofloxacin triggers the transcriptional activation of iron transport genes and also enhances the Fenton reaction.

Therapeutic iminoboronate-based polymersomes with a Cu(ii)-mediated Fenton reaction-enhanced ROS-response

2020 ◽  
Vol 56 (81) ◽  
pp. 12246-12249
Author(s):  
Ruidong Cheng ◽  
Guo Li ◽  
Li Fan ◽  
Jinqiang Jiang ◽  
Yue Zhao
Keyword(s):  
Reactive Oxygen Species ◽  
Fenton Reaction ◽  
Reactive Oxygen ◽  
Oxygen Species

The iminoboronate-terminalized starlike prodrug, N3-(OEG-IBCAPE)4, is prepared to investigate the Cu(ii)-mediated Fenton reaction-enhanced response to reactive oxygen species.

scholarly journals Role of mitochondrial superoxide dismutase in contraction-induced generation of reactive oxygen species in skeletal muscle extracellular space

2004 ◽  
Vol 286 (5) ◽  
pp. C1152-C1158 ◽  
Author(s):  
A. McArdle ◽  
J. van der Meulen ◽  
G. L. Close ◽  
D. Pattwell ◽  
H. Van Remmen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydroxyl Radical ◽  
Hydroxyl Radicals ◽  
Extracellular Space ◽  
Superoxide Anion ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Superoxide Anions ◽  
Wild Type ◽  
Mnsod Activity

Contractions of skeletal muscles produce increases in concentrations of superoxide anions and activity of hydroxyl radicals in the extracellular space. The sources of these reactive oxygen species are not clear. We tested the hypothesis that, after a demanding isometric contraction protocol, the major source of superoxide and hydroxyl radical activity in the extracellular space of muscles is mitochondrial generation of superoxide anions and that, with a reduction in MnSOD activity, concentration of superoxide anions in the extracellular space is unchanged but concentration of hydroxyl radicals is decreased. For gastrocnemius muscles from adult (6–8 mo old) wild-type ( Sod2+/+) mice and knockout mice heterozygous for the MnSOD gene ( Sod2+/-), concentrations of superoxide anions and hydroxyl radical activity were measured in the extracellular space by microdialysis. A 15-min protocol of 180 isometric contractions induced a rapid, equivalent increase in reduction of cytochrome c as an index of superoxide anion concentrations in the extracellular space of Sod2+/+ and Sod2+/- mice, whereas hydroxyl radical activity measured by formation of 2,3-dihydroxybenzoate from salicylate increased only in the extracellular space of muscles of Sod2+/+ mice. The lack of a difference in increase in superoxide anion concentration in the extracellular space of Sod2+/+ and Sod2+/- mice after the contraction protocol supported the hypothesis that superoxide anions were not directly derived from mitochondria. In contrast, the data obtained suggest that the increase in hydroxyl radical concentration in the extracellular space of muscles from wild-type mice after the contraction protocol most likely results from degradation of hydrogen peroxide generated by MnSOD activity.

scholarly journals Insight on the generation of reactive oxygen species in the CaO2/Fe(II) Fenton system and the hydroxyl radical advancing strategy

2018 ◽  
Vol 353 ◽  
pp. 657-665 ◽  
Author(s):  
Yunfei Xue ◽  
Qian Sui ◽  
Mark L. Brusseau ◽  
Xiang Zhang ◽  
Zhaofu Qiu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydroxyl Radical ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Fenton System

scholarly journals Reactivity of Several Reactive Oxygen Species (ROS) with the Sesquiterpene Cacalol

2008 ◽  
Vol 3 (4) ◽  
pp. 1934578X0800300
Author(s):  
Manuel Jiménez-Estrada ◽  
Ricardo Reyes-Chilpa ◽  
Arturo Navarro-Ocaña ◽  
Daniel Arrieta-Báez
Keyword(s):  
Reactive Oxygen Species ◽  
Hydroxyl Radical ◽  
Singlet Oxygen ◽  
Furan Ring ◽  
Reactive Oxygen ◽  
Antioxidant Effect ◽  
Oxygen Species ◽  
Site Reaction ◽  
Hexanedioic Acid ◽  
Antioxidant Effects

To analyze the antioxidant effects of cacalol we determined its reactivity with different reactive oxygen species (ROS). Cacalol gave rise to cacalone by a specific site reaction with a hydroxyl radical. Singlet oxygen reacted only with the double bond of the furan ring, causing its rupture. On the other hand, ozone reacted with all double bonds in cacalol affording 2-methyl-hexanedioic acid as an end product. No reaction was observed with either superoxide or hydrogen peroxide. The potential antioxidant effect of cacalol as a scavenger of hydroxyl radical and singlet oxygen could be related to its function in the plant roots.

Sign in / Sign up

Export Citation Format

Share Document