A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity

2020 ◽  
Vol 56 (40) ◽  
pp. 5358-5361
Author(s):  
Yanglingzhi Chen ◽  
Keiji Murayama ◽  
Hiromu Kashida ◽  
Yukiko Kamiya ◽  
Hiroyuki Asanuma
Keyword(s):  
High Sensitivity ◽  
High Signal ◽  
Duplex Dna ◽  
Specific Detection ◽  
Background Ratio ◽  
Double Stranded Dna ◽  
Linear Probe ◽  
Specific Manner ◽  
Triplex Forming Oligonucleotide

We have developed a d-threoninol-based triplex-forming oligonucleotide (TFO) linear probe that can fluorescently detect target double-stranded DNA in a sequence-specific manner by forming stable triplex with high signal/background ratio.

Computationally Assisted Design of High Signal-to-Noise Photoinduced Electron Transfer-Based Voltage-Sensitive Dyes

2020 ◽  
Author(s):  
Rishikesh Kulkarni ◽  
Anneliese Gest ◽  
Chun Kei Lam ◽  
Benjamin Raliski ◽  
Feroz James ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Dft Calculations ◽  
Photoinduced Electron Transfer ◽  
Density Functional ◽  
Embryonic Stem ◽  
High Sensitivity ◽  
Md Simulations ◽  
High Signal ◽  
Signal To Noise ◽  
Green Fluorescent

<p>High signal-to-noise optical voltage indicators will enable simultaneous interrogation of membrane potential in large ensembles of neurons. However, design principles for voltage sensors with high sensitivity and brightness remain elusive, limiting the applicability of voltage imaging. In this paper, we use molecular dynamics (MD) simulations and density functional theory (DFT) calculations to guide the design of a bright and sensitive green-fluorescent voltage-sensitive fluorophore, or VoltageFluor (VF dye), that uses photoinduced electron transfer (PeT) as a voltage-sensing mechanism. MD simulations predict an 11% increase in sensitivity due to membrane orientation, while DFT calculations predict an increase in fluorescence quantum yield, but a decrease in sensitivity due to a decrease in rate of PeT. We confirm these predictions by synthesizing a new VF dye and demonstrating that it displays the expected improvements by doubling the brightness and retaining similar sensitivity to prior VF dyes. Combining theoretical predictions and experimental validation has resulted in the synthesis of the highest signal-to-noise green VF dye to date. We use this new voltage indicator to monitor the electrophysiological maturation of human embryonic stem cell-derived medium spiny neurons. </p>

scholarly journals Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV

2021 ◽  
Vol 9 (6) ◽  
pp. 1234
Author(s):  
Dejan Vidanović ◽  
Bojana Tešović ◽  
Milanko Šekler ◽  
Zoran Debeljak ◽  
Nikola Vasković ◽  
...  
Keyword(s):  
Diagnostic Performance ◽  
High Sensitivity ◽  
Animal Disease ◽  
Wild Type ◽  
Notifiable Disease ◽  
Specific Detection ◽  
Lumpy Skin Disease ◽  
Systemic Reactions ◽  
Vaccination Campaigns ◽  
Significant Health

Lumpy skin disease (LSD) is an important animal disease with significant health and economic impacts. It is considered a notifiable disease by the OIE. Attenuated strains of LSDV have been successfully used as vaccines (LAV) but can also produce mild or systemic reactions. Vaccination campaigns using LAVs are therefore only viable if accompanying DIVA assays are available. Two DIVA qPCR assays able to distinguish Neethling-based LAVs and wild-type LSDV were developed. Upon validation, both assays were shown to have high sensitivity and specificity with a diagnostic performance comparable to other published DIVA assays. This confirmed their potential as reliable tools to confirm infection in animals during vaccination campaigns based on Neethling vaccine strains.

scholarly journals Mid-infrared photoacoustic gas monitoring driven by a gas-filled hollow-core fiber laser

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yazhou Wang ◽  
Yuyang Feng ◽  
Abubakar I. Adamu ◽  
Manoj K. Dasa ◽  
J. E. Antonio-Lopez ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Laser Source ◽  
High Signal ◽  
Mid Infrared ◽  
Raman Fiber Laser ◽  
Custom Made ◽  
Core Fiber ◽  
Detection Technologies

AbstractDevelopment of novel mid-infrared (MIR) lasers could ultimately boost emerging detection technologies towards innovative spectroscopic and imaging solutions. Photoacoustic (PA) modality has been heralded for years as one of the most powerful detection tools enabling high signal-to-noise ratio analysis. Here, we demonstrate a novel, compact and sensitive MIR-PA system for carbon dioxide (CO2) monitoring at its strongest absorption band by combining a gas-filled fiber laser and PA technology. Specifically, the PA signals were excited by a custom-made hydrogen (H2) based MIR Raman fiber laser source with a pulse energy of ⁓ 18 μJ, quantum efficiency of ⁓ 80% and peak power of ⁓ 3.9 kW. A CO2 detection limit of 605 ppbv was attained from the Allan deviation. This work constitutes an alternative method for advanced high-sensitivity gas detection.

scholarly journals Methods for Voltage-Sensitive Dye Imaging of Rat Cortical Activity With High Signal-to-Noise Ratio

2007 ◽  
Vol 98 (1) ◽  
pp. 502-512 ◽  
Author(s):  
Michael T. Lippert ◽  
Kentaroh Takagaki ◽  
Weifeng Xu ◽  
Xiaoying Huang ◽  
Jian-Young Wu
Keyword(s):  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
Field Potential ◽  
High Sensitivity ◽  
High Dynamic Range ◽  
High Signal ◽  
Blue Dye ◽  
Voltage Sensitive Dye ◽  
Imaging Device

We describe methods to achieve high sensitivity in voltage-sensitive dye (VSD) imaging from rat barrel and visual cortices in vivo with the use of a blue dye RH1691 and a high dynamic range imaging device (photodiode array). With an improved staining protocol and an off-line procedure to remove pulsation artifact, the sensitivity of VSD recording is comparable with that of local field potential recording from the same location. With this sensitivity, one can record from ∼500 individual detectors, each covering an area of cortical tissue 160 μm in diameter (total imaging field ∼4 mm in diameter) and a temporal resolution of 1,600 frames/s, without multiple-trial averaging. We can record 80–100 trials of intermittent 10-s trials from each imaging field before the VSD signal reduces to one half of its initial amplitude because of bleaching and wash-out. Taken together, the methods described in this report provide a useful tool for visualizing evoked and spontaneous waves from rodent cortex.

Specific Detection of Staphylococcus Enterotoxin B in Milk Using Piezoresistive Cantilever Aptasensor

2015 ◽  
Vol 645-646 ◽  
pp. 1298-1302
Author(s):  
Rui Zhao ◽  
Yong Zheng Wen ◽  
Wei Ma ◽  
Jian Cheng Yang ◽  
Xiao Mei Yu
Keyword(s):  
High Sensitivity ◽  
Rapid Response ◽  
Experimental Results ◽  
Quick Response ◽  
Specific Detection ◽  
Detection Range ◽  
Coated Surface ◽  
Enterotoxin B ◽  
Food Commodities ◽  
Staphylococcus Enterotoxin B

In this paper, an aptasensor with high sensitivity and rapid response was developed for the detection of staphylococcus enterotoxin B (SEB) by using thiol-modified piezoresistive cantilever. Thiol-modified aptamers, acting as the functionalized sensing elements, were immobilized on the nanogold-coated surface of the sensing cantilever as agents for detecting SEB. By using the functionalized aptasensors, different concentrations of SEB were detected with a wide detection range of 6-100 ng/mL and a quick response in milk. The experimental results indicated that the cantilever-based aptasensors had sufficient sensitivity for the detection of SEB in real food commodities and might provide an economical platform for on-site detections of different toxicants with the advantages of portability, high sensitivity, and rapid response.

Thioflavin T specifically brightening “Guanine Island” in duplex-DNA: a novel fluorescent probe for single-nucleotide mutation

The Analyst ◽  
2019 ◽  
Vol 144 (7) ◽  
pp. 2284-2290 ◽  
Author(s):  
Wei Zhou ◽  
Ze Yu ◽  
Ge Ma ◽  
Tian Jin ◽  
Yunchao Li ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Thioflavin T ◽  
Duplex Dna ◽  
Single Nucleotide ◽  
Single Nucleotide Mutation ◽  
Double Stranded Dna ◽  
Nucleotide Mutation

Here, we found that Thioflavin T (ThT) could specifically bind with a G-GGG unit (named as “Guanine Island”) in double stranded DNA (ds-DNA).

A Carbon-Based DNA Framework Nano–Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio

ACS Sensors ◽  
2020 ◽  
Vol 5 (12) ◽  
pp. 3979-3987
Author(s):  
Jing Su ◽  
Wenhan Liu ◽  
Shixing Chen ◽  
Wangping Deng ◽  
Yanzhi Dou ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
High Signal ◽  
Signal To Noise ◽  
Noise Ratio ◽  
Carbon Based

Bis-intercalation-triggered fluorescence: Specific detection of double stranded DNA and AT content estimation

The Analyst ◽  
2004 ◽  
Vol 129 (10) ◽  
pp. 886 ◽  
Author(s):  
Hiroyuki Ueyama ◽  
Keiji Mizuki ◽  
Takahiko Nojima ◽  
Shigeori Takenaka
Keyword(s):  
Specific Detection ◽  
Double Stranded Dna

SPECIFIC DETECTION OF FIBRIN IN HUMAN TISSUES BY A NEW IMMUNOHISTOCHEMICAL TECHNIQUE.

1987 ◽  
Author(s):  
A Bini ◽  
R Mesa-Tejada ◽  
J Fenoglio ◽  
B Kudryk ◽  
K L Kaplan
Keyword(s):  
Molecular Form ◽  
High Sensitivity ◽  
Polyclonal Antiserum ◽  
Immunoperoxidase Technique ◽  
Pericellular Matrix ◽  
Specific Detection ◽  
Human Biopsy ◽  
Embryonic Origin ◽  
Avidin Biotin Complex

Human biopsy (30), surgical (50) and autopsy (14) specimens of different embryonic origin (skin, blood vessel, kidney, lymph nodes, prostate, lung, liver, and intestine) were stained by the avidin-biotin complex immunoperoxidase technique (ABC-IP) with monoclonal antibodies (MAbs). MAb T2G1 (recognizes 315-42 and detects fibrin II in tissues), MAb I8C6 (recognizes BS1-42 and indicates fibrinogen and fibrin I), MAb GC4 (specific for fragments D and D-D), and a polyclonal antiserum for fibrinogen. The method can be applied to frozen or Boilin’s fixed paraffin embedded tissues with good preservation of morphology and high sensitivity at the light microscopy level. The results were compared with conventional histochemical stains currently used in surgical pathology to demonstrate fibrin deposits in tissues. These stains are based on the acidophilic properties of fibrin (Fraser-Lendrum for “more recent” and Mallory’s PTAH for “older” fibrin). ABC-IP staining with MAb T2G1 clearly detected fibrin in areas where Lendrum and PTAH failed to reveal fibrin deposits, e.g., in the intercellular and pericellular matrix, as well as in areas where staining occurred with conventional techniques, indicating greater sensitivity of the ABC-IP method. Fibrin was specifically detected in strands or clumps in some areas of inflammation and granulation tissue and seemed to be associated with platelets and macrophages. Moreover, ABC-IP with MAb I8C6 and MAb GC4 permits the distinction between fibrinogen or fibrin I, and D and D dimer which are poorly reactive with the Lendrum and PTAH methods. The polyclonal antiserum for fibrinogen showed reactivity with all the material stained with the MAbs and with some additional areas due to the epitopes of fibrinogen and fibrin not detected by the monoclonals. The ABC-IP technique with MAbs allows specific demonstration of fibrin deposits in tissues. Moreover, these results indicate that this method facilitates the correlation of the morphologic appearance of fibrinogen) -related deposits in tissues with their molecular form and will be useful to elucidate the role of fibrin in diseases such as atherosclerosis, kidney disease, and tumors.

Efficient red luminogen with aggregation-induced emission for in vivo three-photon brain vascular imaging

2020 ◽  
Vol 4 (6) ◽  
pp. 1634-1642 ◽  
Author(s):  
Haiyan Tian ◽  
Dongyu Li ◽  
Xi Tang ◽  
Yubo Zhang ◽  
Zhiyong Yang ◽  
...  
Keyword(s):  
High Resolution ◽  
Penetration Depth ◽  
Vascular Imaging ◽  
Quantum Yields ◽  
High Signal ◽  
Aggregation Induced Emission ◽  
Background Ratio ◽  
High Quantum

DCPE-TPA exhibits three morphologies with high quantum yields and nanoparticles of DCPE-TPA are utilized for in vivo 3PF imaging, achieving a penetration depth of 300 μm with a high resolution of 1.8 μm and a high signal-to-background ratio of 14.

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