scholarly journals Engineering of Biofilms with a Glycosylation Circuit for Biomaterial Applications

2021 ◽  
Author(s):  
Ebru Sahin Kehribar ◽  
Musa E İsilak ◽  
Eray U. Bozkurt ◽  
Jozef Adamcik ◽  
Raffaele Mezzenga ◽  
...  

Glycosylation is a crucial post-translational modification for a wide range of functionalities. Adhesive protein-based biomaterials in nature rely on heavily glycosylated proteins such as spider silk and mussel adhesive proteins....

Soft Matter ◽  
2017 ◽  
Vol 13 (42) ◽  
pp. 7704-7716 ◽  
Author(s):  
Hyo Jeong Kim ◽  
Byeongseon Yang ◽  
Tae Yoon Park ◽  
Seonghye Lim ◽  
Hyung Joon Cha

Recombinant mussel adhesive protein-based complex coacervates are an effective underwater adhesive and have significant potential in practical applications that require underwater adhesion.


RSC Advances ◽  
2016 ◽  
Vol 6 (90) ◽  
pp. 87044-87048 ◽  
Author(s):  
Hyunjung Lee ◽  
Yu-Mi Ha ◽  
Sang Hyun Lee ◽  
Young-il Ko ◽  
Hiroyuki Muramatsu ◽  
...  

We demonstrated the pH-mediated self-healing performance of an electrically conductive gel comprising mussel adhesive proteins (MAPs) and carbon nanotubes (CNTs).


Author(s):  
Ingo Grunwald ◽  
Klaus Rischka ◽  
Stefan M Kast ◽  
Thomas Scheibel ◽  
Hendrik Bargel

Proteins are ubiquitous biopolymers that adopt distinct three-dimensional structures and fulfil a multitude of elementary functions in organisms. Recent systematic studies in molecular biology and biotechnology have improved the understanding of basic functional and architectural principles of proteins, making them attractive candidates as concept generators for technological development in material science, particularly in biomedicine and nano(bio)technology. This paper highlights the potential of molecular biomimetics in mimicking high-performance proteins and provides concepts for applications in four case studies, i.e. spider silk, antifreeze proteins, blue mussel adhesive proteins and viral ion channels.


2017 ◽  
Vol 9 (8) ◽  
pp. 7866-7872 ◽  
Author(s):  
Michael A. North ◽  
Chelsey A. Del Grosso ◽  
Jonathan J. Wilker

2004 ◽  
Vol 70 (6) ◽  
pp. 3352-3359 ◽  
Author(s):  
Dong Soo Hwang ◽  
Hyo Jin Yoo ◽  
Jong Hyub Jun ◽  
Won Kyu Moon ◽  
Hyung Joon Cha

ABSTRACT Mussel adhesive proteins have been suggested as a basis for environmentally friendly adhesives for use in aqueous conditions and in medicine. However, attempts to produce functional and economical recombinant mussel adhesive proteins (mainly foot protein type 1) in several systems have failed. Here, the cDNA coding for Mytilus galloprovincialis foot protein type 5 (Mgfp-5) was isolated for the first time. Using this cDNA, we produced a recombinant Mgfp-5 fused with a hexahistidine affinity ligand, which was expressed in a soluble form in Escherichia coli and was highly purified using affinity chromatography. The adhesive properties of purified recombinant Mgfp-5 were compared with the commercial extracted mussel adhesive Cell-Tak by investigating adhesion force using atomic force microscopy, material surface coating, and quartz crystal microbalance. Even though further macroscale assays are needed, these microscale assays showed that recombinant Mgfp-5 has significant adhesive ability and may be useful as a bioadhesive in medical or underwater environments.


2020 ◽  
Vol 164 ◽  
pp. 108351 ◽  
Author(s):  
Jie Cheng ◽  
Sulin Chen ◽  
Fan Zhang ◽  
Bin Shen ◽  
Xinchun Lu ◽  
...  

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