High Strength Underwater Bonding with Polymer Mimics of Mussel Adhesive Proteins

2017 ◽  
Vol 9 (8) ◽  
pp. 7866-7872 ◽  
Author(s):  
Michael A. North ◽  
Chelsey A. Del Grosso ◽  
Jonathan J. Wilker
Keyword(s):  
High Strength ◽  
Mussel Adhesive Proteins ◽  
Mussel Adhesive ◽  
Adhesive Proteins

scholarly journals Engineering of Biofilms with a Glycosylation Circuit for Biomaterial Applications

2021 ◽  
Author(s):  
Ebru Sahin Kehribar ◽  
Musa E İsilak ◽  
Eray U. Bozkurt ◽  
Jozef Adamcik ◽  
Raffaele Mezzenga ◽  
...  
Keyword(s):  
Spider Silk ◽  
Post Translational Modification ◽  
Adhesive Protein ◽  
Mussel Adhesive Proteins ◽  
Wide Range ◽  
Mussel Adhesive ◽  
Adhesive Proteins ◽  
Glycosylated Proteins

Glycosylation is a crucial post-translational modification for a wide range of functionalities. Adhesive protein-based biomaterials in nature rely on heavily glycosylated proteins such as spider silk and mussel adhesive proteins....

Complex coacervates based on recombinant mussel adhesive proteins: their characterization and applications

Soft Matter ◽  
2017 ◽  
Vol 13 (42) ◽  
pp. 7704-7716 ◽  
Author(s):  
Hyo Jeong Kim ◽  
Byeongseon Yang ◽  
Tae Yoon Park ◽  
Seonghye Lim ◽  
Hyung Joon Cha
Keyword(s):  
Practical Applications ◽  
Adhesive Protein ◽  
Underwater Adhesion ◽  
Significant Potential ◽  
Mussel Adhesive Proteins ◽  
Mussel Adhesive Protein ◽  
Mussel Adhesive ◽  
Adhesive Proteins

Recombinant mussel adhesive protein-based complex coacervates are an effective underwater adhesive and have significant potential in practical applications that require underwater adhesion.

scholarly journals Expression of Functional Recombinant Mussel Adhesive Protein Mgfp-5 in Escherichia coli

2004 ◽  
Vol 70 (6) ◽  
pp. 3352-3359 ◽  
Author(s):  
Dong Soo Hwang ◽  
Hyo Jin Yoo ◽  
Jong Hyub Jun ◽  
Won Kyu Moon ◽  
Hyung Joon Cha
Keyword(s):  
Escherichia Coli ◽  
Adhesion Force ◽  
Soluble Form ◽  
Material Surface ◽  
Adhesive Properties ◽  
Affinity Ligand ◽  
Mussel Adhesive Proteins ◽  
Mussel Adhesive ◽  
Aqueous Conditions ◽  
Adhesive Proteins

ABSTRACT Mussel adhesive proteins have been suggested as a basis for environmentally friendly adhesives for use in aqueous conditions and in medicine. However, attempts to produce functional and economical recombinant mussel adhesive proteins (mainly foot protein type 1) in several systems have failed. Here, the cDNA coding for Mytilus galloprovincialis foot protein type 5 (Mgfp-5) was isolated for the first time. Using this cDNA, we produced a recombinant Mgfp-5 fused with a hexahistidine affinity ligand, which was expressed in a soluble form in Escherichia coli and was highly purified using affinity chromatography. The adhesive properties of purified recombinant Mgfp-5 were compared with the commercial extracted mussel adhesive Cell-Tak by investigating adhesion force using atomic force microscopy, material surface coating, and quartz crystal microbalance. Even though further macroscale assays are needed, these microscale assays showed that recombinant Mgfp-5 has significant adhesive ability and may be useful as a bioadhesive in medical or underwater environments.

Corrosion- and wear-resistant composite film of graphene and mussel adhesive proteins on carbon steel

2020 ◽  
Vol 164 ◽  
pp. 108351 ◽  
Author(s):  
Jie Cheng ◽  
Sulin Chen ◽  
Fan Zhang ◽  
Bin Shen ◽  
Xinchun Lu ◽  
...  
Keyword(s):  
Carbon Steel ◽  
Composite Film ◽  
Corrosion And Wear ◽  
Wear Resistant ◽  
Mussel Adhesive Proteins ◽  
Mussel Adhesive ◽  
Adhesive Proteins

scholarly journals Optimization of Polydopamine Coatings onto Poly–ε–Caprolactone Electrospun Fibers for the Fabrication of Bio-Electroconductive Interfaces

2020 ◽  
Vol 11 (1) ◽  
pp. 19 ◽  
Author(s):  
Simona Zuppolini ◽  
Iriczalli Cruz-Maya ◽  
Vincenzo Guarino ◽  
Anna Borriello
Keyword(s):  
Self Assembly ◽  
Fluid Dynamic ◽  
Electrospun Fibers ◽  
Oxidation Reactions ◽  
Mussel Adhesive Proteins ◽  
Mussel Adhesive ◽  
Adhesive Proteins ◽  
Conductive Properties

In recent years, mussel adhesive proteins have attracted much attention because they can form strong adhesive interface interactions with various substrates in a wet environment. Inspired by their catechol- and amine-based molecular structure, polydopamine (PDA), a dopamine derived synthetic eumelanin polymer, was recognized as a suitable bio-interface coating. PDA was successfully used to improve adhesion due to the availability of copious functional groups for covalently immobilizing biomolecules and anchoring reactive species and ions. Recently, it has been demonstrated that PDA and its derivatives can be successfully used for the surface modification of implants interfaces to modulate in vitro cellular responses in order to enhance the in vivo functionality of biomedical implants (i.e., prosthesis). Herein, we propose the development of multifunctional scaffolds based on polyε–caprolactone (PCL) electrospun fibers coated with PDA via electro fluid dynamic methods, by optimizing polymerization/oxidation reactions capable of driving PDA self–assembly, and, ultimately, investigating the effects on cell response. Morphological analyses have confirmed the possibility to obtain different surface topographies as a function of the coating process while in vitro studies proved the ability of PDA coating to interact with cells no compromising in vitro viability. In perspective, in vitro conductive properties of fibers will be further investigated in order to validate their promising use as bioconductive interfaces for tissue engineering applications.

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