3D printed gelatin/hydroxyapatite scaffolds for stem cell chondrogenic differentiation and articular cartilage repair

2021 ◽  
Author(s):  
Jianghong Huang ◽  
Zhiwang Huang ◽  
Yujie Liang ◽  
Weihao Yuan ◽  
Liming Bian ◽  
...  
Keyword(s):  
Stem Cell ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Chondrogenic Differentiation ◽  
Pig Model ◽  
Cartilage Defects ◽  
Knee Cartilage ◽  
Articular Cartilage Repair ◽  
3D Printed

The hUCB-MSC-laden 3D printed gelatin/HAP scaffold effectively repairs knee cartilage defects in a pig model.

scholarly journals 3D Printing of Collagen/Oligomeric Proanthocyanidin/Oxidized Hyaluronic Acid Composite Scaffolds for Articular Cartilage Repair

Polymers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 3123
Author(s):  
Chung-Fei Lee ◽  
Yung-Heng Hsu ◽  
Yu-Chien Lin ◽  
Thu-Trang Nguyen ◽  
Hsiang-Wen Chen ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Articular Cartilage ◽  
3D Printing ◽  
Cartilage Repair ◽  
Joint Disease ◽  
Tissue Loss ◽  
Cartilage Defects ◽  
Natural Polymers ◽  
Composite Scaffolds ◽  
Articular Cartilage Repair

Articular cartilage defects affect millions of people worldwide, including children, adolescents, and adults. Progressive wear and tear of articular cartilage can lead to progressive tissue loss, further exposing the bony ends and leaving them unprotected, which may ultimately cause osteoarthritis (degenerative joint disease). Unlike other self-repairing tissues, cartilage has a low regenerative capacity; once injured, the cartilage is much more difficult to heal. Consequently, developing methods to repair this defect remains a challenge in clinical practice. In recent years, tissue engineering applications have employed the use of three-dimensional (3D) porous scaffolds for growing cells to regenerate damaged cartilage. However, these scaffolds are mainly chemically synthesized polymers or are crosslinked using organic solvents. Utilizing 3D printing technologies to prepare biodegradable natural composite scaffolds could replace chemically synthesized polymers with more natural polymers or low-toxicity crosslinkers. In this study, collagen/oligomeric proanthocyanidin/oxidized hyaluronic acid composite scaffolds showing high biocompatibility and excellent mechanical properties were prepared. The compressive strengths of the scaffolds were between 0.25–0.55 MPa. Cell viability of the 3D scaffolds reached up to 90%, which indicates that they are favorable surfaces for the deposition of apatite. An in vivo test was performed using the Sprague Dawley (SD) rat skull model. Histological images revealed signs of angiogenesis and new bone formation. Therefore, 3D collagen-based scaffolds can be used as potential candidates for articular cartilage repair.

Activation of the Mouse Resident Skeletal Stem Cell for Articular Cartilage Repair

2017 ◽  
Vol 225 (4) ◽  
pp. S160
Author(s):  
Matthew P. Murphy ◽  
Michael Lopez ◽  
Ryan C. Ransom ◽  
Owen Marecic ◽  
Gunsagar S. Gulati ◽  
...  
Keyword(s):  
Stem Cell ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Articular Cartilage Repair ◽  
Skeletal Stem Cell

scholarly journals A comparison of BMP2 delivery by coacervate and gene therapy for promoting human muscle-derived stem cell-mediated articular cartilage repair

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Xueqin Gao ◽  
Haizi Cheng ◽  
Hassan Awada ◽  
Ying Tang ◽  
Sarah Amra ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Raman Spectroscopy ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Chondrogenic Differentiation ◽  
Human Muscle ◽  
Articular Cartilage Repair ◽  
Sustain Release

Abstract Background Osteoarthritis and cartilage injury treatment is an unmet clinical need. Therefore, development of new approaches to treat these diseases is critically needed. Previous work in our laboratory has shown that murine muscle-derived stem cells (MDSCs) can efficiently repair articular cartilage in an osteochondral and osteoarthritis model. However, the cartilage repair capacity of human muscle-derived stem cells has not been studied which prompt this study. Method In this study, we tested the in vitro chondrogenesis ability of six populations of human muscle-derived stem cells (hMDSCs), before and after lenti-BMP2/GFP transduction using pellet culture and evaluated chondrogenic differentiation of via histology and Raman spectroscopy. We further compared the in vivo articular cartilage repair of hMDSCs stimulated with BMP2 delivered through coacervate sustain release technology and lenti-viral gene therapy-mediated gene delivery in a monoiodoacetate (MIA)-induced osteoarthritis (OA) model. We used microCT and histology to evaluate the cartilage repair. Results We observed that all hMDSCs were able to undergo chondrogenic differentiation in vitro. As expected, lenti-BMP2/GFP transduction further enhanced the chondrogenic differentiation capacities of hMDSCs, as confirmed by Alcian blue and Col2A1staining as well as Raman spectroscopy analysis. We observed through micro-CT scanning, Col2A1 staining, and histological analyses that delivery of BMP2 with coacervate could achieve a similar articular cartilage repair to that mediated by hMDSC-LBMP2/GFP. We also found that the addition of soluble fms-like tyrosine kinase-1 (sFLT-1) protein further improved the regenerative potential of hMDSCs/BMP2 delivered through the coacervate sustain release technology. Donor cells did not primarily contribute to the repaired articular cartilage since most of the repair cells are host derived as indicated by GFP staining. Conclusions We conclude that the delivery of hMDSCs and BMP2 with the coacervate technology can achieve a similar cartilage repair relative to lenti-BMP2/GFP-mediated gene therapy. The use of coacervate technology to deliver BMP2/sFLT1 with hMDSCs for cartilage repair holds promise for possible clinical translation into an effective treatment modality for osteoarthritis and traumatic cartilage injury.

Current Progress in Stem Cell-Based Gene Therapy for Articular Cartilage Repair

2015 ◽  
Vol 10 (2) ◽  
pp. 121-131 ◽  
Author(s):  
Janina Frisch ◽  
Jagadeesh Venkatesan ◽  
Ana Rey-Rico ◽  
Henning Madry ◽  
Magali Cucchiarini
Keyword(s):  
Gene Therapy ◽  
Stem Cell ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Articular Cartilage Repair

scholarly journals Articular Cartilage Repair of the Knee in Children and Adolescents

2018 ◽  
Vol 6 (3) ◽  
pp. 232596711876019 ◽  
Author(s):  
Gian M. Salzmann ◽  
Philipp Niemeyer ◽  
Alfred Hochrein ◽  
Martin J. Stoddart ◽  
Peter Angele
Keyword(s):  
Articular Cartilage ◽  
Children And Adolescents ◽  
Cartilage Repair ◽  
Joint Surface ◽  
Cartilage Defects ◽  
Complication Rates ◽  
Articular Cartilage Repair ◽  
Biological Potential ◽  
Cartilage Injuries ◽  
Articular Cartilage Injuries

Articular cartilage predominantly serves a biomechanical function, which begins in utero and further develops during growth and locomotion. With regard to its 2-tissue structure (chondrocytes and matrix), the regenerative potential of hyaline cartilage defects is limited. Children and adolescents are increasingly suffering from articular cartilage and osteochondral deficiencies. Traumatic incidents often result in damage to the joint surfaces, while repetitive microtrauma may cause osteochondritis dissecans. When compared with their adult counterparts, children and adolescents have a greater capacity to regenerate articular cartilage defects. Even so, articular cartilage injuries in this age group may predispose them to premature osteoarthritis. Consequently, surgery is indicated in young patients when conservative measures fail. The operative techniques for articular cartilage injuries traditionally performed in adults may be performed in children, although an individualized approach must be tailored according to patient and defect characteristics. Clear guidelines for defect dimension–associated techniques have not been reported. Knee joint dimensions must be considered and correlated with respect to the cartilage defect size. Particular attention must be given to the subchondral bone, which is frequently affected in children and adolescents. Articular cartilage repair techniques appear to be safe in this cohort of patients, and no differences in complication rates have been reported when compared with adult patients. Particularly, autologous chondrocyte implantation has good biological potential, especially for large-diameter joint surface defects.

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