scholarly journals Articular cartilage repair using dedifferentiated articular chondrocytes and bone morphogenetic protein 4 in a rabbit model of articular cartilage defects

2008 ◽  
Vol 58 (4) ◽  
pp. 1067-1075 ◽  
Author(s):  
Lin Lin ◽  
Chunyan Zhou ◽  
Xuelei Wei ◽  
Yu Hou ◽  
Liheng Zhao ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Bone Morphogenetic Protein ◽  
Cartilage Repair ◽  
Articular Chondrocytes ◽  
Rabbit Model ◽  
Cartilage Defects ◽  
Bone Morphogenetic Protein 4 ◽  
Articular Cartilage Repair ◽  
Morphogenetic Protein ◽  
Articular Cartilage Defects

Facilitating In Vivo Articular Cartilage Repair by Tissue-Engineered Cartilage Grafts Produced From Auricular Chondrocytes

2017 ◽  
Vol 46 (3) ◽  
pp. 713-727 ◽  
Author(s):  
Chin-Chean Wong ◽  
Chih-Hwa Chen ◽  
Li-Hsuan Chiu ◽  
Yang-Hwei Tsuang ◽  
Meng-Yi Bai ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Articular Chondrocytes ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Articular Cartilage Repair ◽  
3 Dimensional ◽  
Cell Conversion

Background: Insufficient cell numbers still present a challenge for articular cartilage repair. Converting heterotopic auricular chondrocytes by extracellular matrix may be the solution. Hypothesis: Specific extracellular matrix may convert the phenotype of auricular chondrocytes toward articular cartilage for repair. Study Design: Controlled laboratory study. Methods: For in vitro study, rabbit auricular chondrocytes were cultured in monolayer for several passages until reaching status of dedifferentiation. Later, they were transferred to chondrogenic type II collagen (Col II)–coated plates for further cell conversion. Articular chondrogenic profiles, such as glycosaminoglycan deposition, articular chondrogenic gene, and protein expression, were evaluated after 14-day cultivation. Furthermore, 3-dimensional constructs were fabricated using Col II hydrogel-associated auricular chondrocytes, and their histological and biomechanical properties were analyzed. For in vivo study, focal osteochondral defects were created in the rabbit knee joints, and auricular Col II constructs were implanted for repair. Results: The auricular chondrocytes converted by a 2-step protocol expressed specific profiles of chondrogenic molecules associated with articular chondrocytes. The histological and biomechanical features of converted auricular chondrocytes became similar to those of articular chondrocytes when cultivated with Col II 3-dimensional scaffolds. In an in vivo animal model of osteochondral defects, the treated group (auricular Col II) showed better cartilage repair than did the control groups (sham, auricular cells, and Col II). Histological analyses revealed that cartilage repair was achieved in the treated groups with abundant type II collagen and glycosaminoglycans syntheses rather than elastin expression. Conclusion: The study confirmed the feasibility of applying heterotopic chondrocytes for cartilage repair via extracellular matrix–induced cell conversion. Clinical Relevance: This study proposes a feasible methodology to convert heterotopic auricular chondrocytes for articular cartilage repair, which may serve as potential alternative sources for cartilage repair.

The Inductive Effect of Bone Morphogenetic Protein-4 on Chondral-Lineage Differentiation and In Situ Cartilage Repair

2010 ◽  
Vol 16 (5) ◽  
pp. 1621-1632 ◽  
Author(s):  
YangZi Jiang ◽  
Long Kun Chen ◽  
Ding Cheng Zhu ◽  
Guo Rong Zhang ◽  
Chao Guo ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Cartilage Repair ◽  
Inductive Effect ◽  
Bone Morphogenetic Protein 4 ◽  
Lineage Differentiation ◽  
Morphogenetic Protein

Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage Defects in a Rabbit Model

2016 ◽  
Vol 34 (11) ◽  
pp. 543-549 ◽  
Author(s):  
Reza Fekrazad ◽  
Mohamadreza Baghaban Eslaminejad ◽  
Arman M. Shayan ◽  
Katayoun A.M. Kalhori ◽  
Fatemeh Mashhadi Abbas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Rabbit Model ◽  
Cartilage Defects ◽  
Articular Cartilage Defects
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