scholarly journals Hybridizing clinical translatability with enzyme-free DNA signal amplifiers: recent advances in nucleic acid detection and imaging

2021 ◽  
Author(s):  
Raina M. Borum ◽  
Jesse V. Jokerst
Keyword(s):  
Nucleic Acid ◽  
Imaging Agents ◽  
Nucleic Acid Detection ◽  
Free Dna ◽  
Recent Advances ◽  
Acid Sensors

Strand amplifying HCR and CHA are adaptable with signalers for novel and clinically translatable nucleic acid sensors and imaging agents.

scholarly journals Recent advances and perspectives of nucleic acid detection for coronavirus

2020 ◽  
Vol 10 (2) ◽  
pp. 97-101 ◽  
Author(s):  
Minzhe Shen ◽  
Ying Zhou ◽  
Jiawei Ye ◽  
Abdu Ahmed Abdullah AL-maskri ◽  
Yu Kang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Detection ◽  
Recent Advances

scholarly journals Field Effect Sensors for Nucleic Acid Detection: Recent Advances and Future Perspectives

Sensors ◽  
2015 ◽  
Vol 15 (5) ◽  
pp. 10380-10398 ◽  
Author(s):  
Bruno Veigas ◽  
Elvira Fortunato ◽  
Pedro Baptista
Keyword(s):  
Nucleic Acid ◽  
Field Effect ◽  
Nucleic Acid Detection ◽  
Future Perspectives ◽  
Recent Advances

scholarly journals Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities

2020 ◽  
Vol 166 ◽  
pp. 112445 ◽  
Author(s):  
Jeanne E. van Dongen ◽  
Johanna T.W. Berendsen ◽  
Renske D.M. Steenbergen ◽  
Rob M.F. Wolthuis ◽  
Jan C.T. Eijkel ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Nucleic Acid Detection ◽  
Recent Advances ◽  
Challenges And Opportunities

Nucleic acid detection on DNA chips using highly reactive and stable diazo-biotins

2008 ◽  
Author(s):  
Alain Laurent ◽  
Arnaud Burr ◽  
Thibault Martin ◽  
Frédéric Lasnet ◽  
Sébastien Hauser ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Detection ◽  
Dna Chips

scholarly journals Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse

Neurogenetics ◽  
2021 ◽  
Author(s):  
Antonia Maletzko ◽  
Jana Key ◽  
Ilka Wittig ◽  
Suzana Gispert ◽  
Gabriele Koepf ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Nucleic Acid ◽  
Inflammatory Responses ◽  
Subcellular Fractionation ◽  
Inflammatory Factors ◽  
Null Mouse ◽  
Loss Of Function ◽  
Response Factors ◽  
Perrault Syndrome ◽  
Acid Sensors

AbstractMitochondrial dysfunction may activate innate immunity, e.g. upon abnormal handling of mitochondrial DNA in TFAM mutants or in altered mitophagy. Recent reports showed that also deletion of mitochondrial matrix peptidase ClpP in mice triggers transcriptional upregulation of inflammatory factors. Here, we studied ClpP-null mouse brain at two ages and mouse embryonal fibroblasts, to identify which signaling pathways are responsible, employing mass spectrometry, subcellular fractionation, immunoblots, and reverse transcriptase polymerase chain reaction. Several mitochondrial unfolded protein response factors showed accumulation and altered migration in blue-native gels, prominently the co-chaperone DNAJA3. Its mitochondrial dysregulation increased also its extra-mitochondrial abundance in the nucleus, a relevant observation given that DNAJA3 modulates innate immunity. Similar observations were made for STAT1, a putative DNAJA3 interactor. Elevated expression was observed not only for the transcription factors Stat1/2, but also for two interferon-stimulated genes (Ifi44, Gbp3). Inflammatory responses were strongest for the RLR pattern recognition receptors (Ddx58, Ifih1, Oasl2, Trim25) and several cytosolic nucleic acid sensors (Ifit1, Ifit3, Oas1b, Ifi204, Mnda). The consistent dysregulation of these factors from an early age might influence also human Perrault syndrome, where ClpP loss-of-function leads to early infertility and deafness, with subsequent widespread neurodegeneration.

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