Peptide amphiphiles with multifunctional fragments promoting cellular uptake and endosomal escape as efficient gene vectors

2015 ◽  
Vol 3 (6) ◽  
pp. 1068-1078 ◽  
Author(s):  
Liang Luan ◽  
Qingbin Meng ◽  
Liang Xu ◽  
Zhao Meng ◽  
Husheng Yan ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cellular Uptake ◽  
Transfection Efficiency ◽  
Endosomal Escape ◽  
Peptide Amphiphiles ◽  
Gene Delivery Vectors ◽  
Efficient Gene ◽  
Gene Vectors ◽  
Lipofectamine 2000

A series of peptides containing multiple functional fragments were designed as gene-delivery vectors with transfection efficiency comparable to Lipofectamine 2000.

scholarly journals Aromatic Thioacetal-Bridged ROS-Responsive Nanoparticles as Novel Gene Delivery Vehicles

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2061 ◽  
Author(s):  
Guo-Qing Lin ◽  
Wen-Jing Yi ◽  
Qiang Liu ◽  
Xue-Jun Yang ◽  
Zhi-Gang Zhao
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Zeta Potentials ◽  
Sar Studies ◽  
Gene Delivery Vectors ◽  
Efficient Gene ◽  
Delivery Vehicles ◽  
Low Cytotoxicity

In this report, a series of polycations are designed and synthesized by conjugating reactive oxygen species (ROS)-responsive thioacetal-linkers to low molecular weight (LMW) polyethylenimine (PEI) via ring-opening polymerization. Their structure–activity relationships (SARs) as gene delivery vectors are systematically studied. Although the MWs of the target polymers are only ~9 KDa, they show good DNA binding ability. The formed polyplexes, which are stable toward serum but decomposed under ROS-conditions, have appropriate sizes (180~300 nm) and positive zeta-potentials (+35~50 mV). In vitro experiments reveal that these materials have low cytotoxicity, and higher transfection efficiency (TE) than controls. Furthermore, the title polymers exhibit excellent serum tolerance. With the present of 10% serum, the TE of the polymers even increases up to 10 times higher than 25 KDa PEI and 9 times higher than Lipofectamine 2000. The SAR studies also reveal that electron-withdrawing groups on the aromatic ring in 4a may benefit to balance between the DNA condensation and release for efficient gene transfection.

Structure–activity relationship studies of symmetrical cationic bolasomes as non-viral gene vectors

2016 ◽  
Vol 4 (33) ◽  
pp. 5575-5584 ◽  
Author(s):  
Zheng Huang ◽  
Yi-Mei Zhang ◽  
Qian Cheng ◽  
Ji Zhang ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Structure Activity Relationship ◽  
Activity Relationship ◽  
Viral Gene ◽  
Structure Activity ◽  
Gene Delivery Vectors ◽  
Gene Vectors

Bolalipids based on lysine or cyclen headgroups were synthesized and their structure–activity relationship as gene delivery vectors was studied.

scholarly journals Cationic lipids with a cyclen headgroup: synthesis and structure–activity relationship studies as non-viral gene vectors

RSC Advances ◽  
2017 ◽  
Vol 7 (30) ◽  
pp. 18681-18689 ◽  
Author(s):  
De-Chun Chang ◽  
Yi-Mei Zhang ◽  
Ji Zhang ◽  
Yan-Hong Liu ◽  
Xiao-Qi Yu
Keyword(s):  
Gene Delivery ◽  
Structure Activity Relationship ◽  
Cationic Lipids ◽  
Activity Relationship ◽  
Viral Gene ◽  
Structure Activity Relationships ◽  
Structure Activity ◽  
Gene Delivery Vectors ◽  
Gene Vectors ◽  
Viral Gene Delivery

The structure–activity relationships of cyclen-based cationic lipids as non-viral gene delivery vectors were studied and clarified.

Flexible cyclic siloxane core enhances the transfection efficiency of polyethylenimine-based non-viral gene vectors

2015 ◽  
Vol 3 (42) ◽  
pp. 8250-8267 ◽  
Author(s):  
Cristina M. Uritu ◽  
Manuela Calin ◽  
Stelian S. Maier ◽  
Corneliu Cojocaru ◽  
Alina Nicolescu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Viral Gene ◽  
Cyclic Siloxane ◽  
Gene Vectors

cD4H–AGE–PEI conjugates, with a favorable balance between hydrophilic and hydrophobic moieties, are promising carriers for gene delivery.

Dry powder aerosols of polyethylenimine (PEI)-based gene vectors mediate efficient gene delivery to the lung

2011 ◽  
Vol 154 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Corinna Pfeifer ◽  
Guenther Hasenpusch ◽  
Senta Uezguen ◽  
Manish Kumar Aneja ◽  
Dietrich Reinhardt ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Dry Powder ◽  
Dry Powder Aerosols ◽  
Efficient Gene ◽  
Gene Vectors ◽  
Powder Aerosols

A dendritic catiomer with an MOF motif for the construction of safe and efficient gene delivery systems

2017 ◽  
Vol 5 (42) ◽  
pp. 8322-8329 ◽  
Author(s):  
Shuqi Dong ◽  
Qixian Chen ◽  
Wei Li ◽  
Zhu Jiang ◽  
Jianbiao Ma ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gold Standard ◽  
Transfection Efficiency ◽  
A549 Cells ◽  
Delivery Systems ◽  
The Core ◽  
Efficient Gene

The dendritic catiomer using biocompatible Zr-MOFs as the core exhibited a markedly higher transfection efficiency and lower cytotoxicity than the commercial gold standard branched PEI25k in A549 cells.

scholarly journals Dendron conjugation to graphene oxide using click chemistry for efficient gene delivery

RSC Advances ◽  
2015 ◽  
Vol 5 (62) ◽  
pp. 50196-50211 ◽  
Author(s):  
Kishor Sarkar ◽  
Giridhar Madras ◽  
Kaushik Chatterjee
Keyword(s):  
Graphene Oxide ◽  
Gene Delivery ◽  
Click Chemistry ◽  
Surface Area ◽  
Cellular Uptake ◽  
Large Surface Area ◽  
Efficient Gene ◽  
Attractive Candidate

Owing to its large surface area and rapid cellular uptake, graphene oxide (GO) is emerging as an attractive candidate material for delivery of drugs and genes.

scholarly journals Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors

2010 ◽  
Vol 5 (11) ◽  
pp. 1804-1811 ◽  
Author(s):  
Min Zhang ◽  
Ya-Nan Xue ◽  
Min Liu ◽  
Ren-Xi Zhuo ◽  
Shi-Wen Huang
Keyword(s):  
Gene Delivery ◽  
Highly Efficient ◽  
Gene Delivery Vectors ◽  
Efficient Gene

Engineered PEI-piperazinyl nanoparticles as efficient gene delivery vectors: evidence from both in vitro and in vivo studies

RSC Advances ◽  
2012 ◽  
Vol 2 (10) ◽  
pp. 4335 ◽  
Author(s):  
Soma Patnaik ◽  
Ritu Goyal ◽  
Sushil K. Tripathi ◽  
Mohammed Arif ◽  
Kailash C. Gupta
Keyword(s):  
Gene Delivery ◽  
In Vivo Studies ◽  
Gene Delivery Vectors ◽  
Efficient Gene
Sign in / Sign up

Export Citation Format

Share Document