Biodegradable nanocomposite fibrous scaffold mediated local delivery of vancomycin for the treatment of MRSA infected experimental osteomyelitis

2020 ◽  
Vol 8 (9) ◽  
pp. 2653-2665 ◽  
Author(s):  
Amit G. Krishnan ◽  
Raja Biswas ◽  
Deepthy Menon ◽  
Manitha B. Nair
Keyword(s):  
Bacterial Infection ◽  
Bone Regeneration ◽  
Revision Surgery ◽  
Composite Scaffold ◽  
Local Delivery ◽  
Fibrous Scaffold ◽  
Functional Composite ◽  
Experimental Osteomyelitis

The study shows the development of a biodegradable bi-functional composite scaffold that can reduce bacterial infection, while promotes bone regeneration in osteomyelitis, without the need for revision surgery.

Carboxymethyl Chitosan/Hydroxyapatite Composite Scaffold for Bone Regeneration

2010 ◽  
Vol 123-125 ◽  
pp. 299-302 ◽  
Author(s):  
Xu Xu Bao ◽  
Yuan Li ◽  
Akira Teramoto ◽  
Koji Abe
Keyword(s):  
Bone Regeneration ◽  
Composite Scaffold ◽  
Carboxymethyl Chitosan ◽  
Fibrous Scaffold ◽  
Modified Chitosan ◽  
Micro Particles ◽  
Proliferation And Differentiation ◽  
Chitosan Scaffolds ◽  
Ft Ir ◽  
Surface Morphologies

Natural bone is a composite mainly made from nano/micro-structure of hydroxyapatite and collagen fibers. For bone regeneration by tissue engineering, it is important to synthesize nano-composites with good biocompatibility, high bioactivity and great bonding property as potentially useful scaffold. In this study, we fabricated chitosan nano-nonwoven scaffold via electrospinning and modified chitosan scaffolds by carboxymethylation (CM). Moreover scaffolds were macerated in SBF (simulated body fluid) to form hydroxyapatite on its surface. Surface morphologies (SEM) showed that nano/micro particles formed on the surface of the carboxymethyl chitosan fibrous scaffold. Results of FT-IR and XRD confirmed that the nano/micro particles were hydroxyapatite crystalline. Moreover by employed mice osteoblast (MC3T3-E1) cell for adhesion, proliferation and differentiation assays, and the hydroxyapatite particles appeared to have a great effect on the late stages of osteoblast behavior (alkaline phosphatase ).

scholarly journals The Role of Alcohol, LPS Toxicity, and ALDH2 in Dental Bony Defects

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 651
Author(s):  
Hsiao-Cheng Tsai ◽  
Che-Hong Chen ◽  
Daria Mochly-Rosen ◽  
Yi-Chen Ethan Li ◽  
Min-Huey Chen
Keyword(s):  
Bone Loss ◽  
Bacterial Infection ◽  
Bone Regeneration ◽  
Alcohol Drinking ◽  
Bacterial Species ◽  
Dominant Negative ◽  
Enzyme Inactivation ◽  
Wild Type ◽  
Micro Computed Tomography ◽  
Dominant Negative Mutation

It is estimated that 560 million people carry an East Asian-specific ALDH2*2 dominant-negative mutation which leads to enzyme inactivation. This common ALDH2 polymorphism has a significant association with osteoporosis. We hypothesized that the ALDH2*2 mutation in conjunction with periodontal Porphyromonas gingivalis bacterial infection and alcohol drinking had an inhibitory effect on osteoblasts and bone regeneration. We examined the prospective association of ALDH2 activity with the proliferation and mineralization potential of human osteoblasts in vitro. The ALDH2 knockdown experiments showed that the ALDH2 knockdown osteoblasts lost their proliferation and mineralization capability. To mimic dental bacterial infection, we compared the dental bony defects in wild-type mice and ALDH2*2 knockin mice after injection with purified lipopolysaccharides (LPS), derived from P. gingivalis which is a bacterial species known to cause periodontitis. Micro-computed tomography (micro-CT) scan results indicated that bone regeneration was significantly affected in the ALDH2*2 knockin mice with about 20% more dental bony defects after LPS injection than the wild-type mice. Moreover, the ALDH2*2 knockin mutant mice had decreased osteoblast growth and more dental bone loss in the upper left jaw region after LPS injection. In conclusion, these results indicated that the ALDH2*2 mutation with alcohol drinking and chronic exposure to dental bacterial-derived toxin increased the risk of dental bone loss.

Enhanced Bone Regeneration Using a ZIF‐8‐loaded Fibrin Composite Scaffold

2021 ◽  
pp. 2100416
Author(s):  
Wentao Shi ◽  
Lu Bian ◽  
Yiqing Wu ◽  
Zhe Wang ◽  
Yao Dai ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Composite Scaffold

scholarly journals Angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration

2015 ◽  
Vol 19 (1) ◽  
Author(s):  
Beom-Su Kim ◽  
Jin-Seong Kim ◽  
Sun-Sik Yang ◽  
Hyung-Woo Kim ◽  
Hun Jun Lim ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Composite Scaffold ◽  
Powder Composite ◽  
Bone Powder ◽  
Vascularized Bone

scholarly journals Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 497 ◽  
Author(s):  
Moumita Ghosh ◽  
Michal Halperin-Sternfeld ◽  
Itzhak Grinberg ◽  
Lihi Adler-Abramovich
Keyword(s):  
Extracellular Matrix ◽  
Bone Regeneration ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Composite Hydrogel ◽  
Bone Tissue Regeneration ◽  
Pcr Analysis ◽  
Alizarin Red ◽  
In Vitro Biocompatibility

The high demand for tissue engineering scaffolds capable of inducing bone regeneration using minimally invasive techniques prompts the need for the development of new biomaterials. Herein, we investigate the ability of Alginate incorporated with the fluorenylmethoxycarbonyl-diphenylalanine (FmocFF) peptide composite hydrogel to serve as a potential biomaterial for bone regeneration. We demonstrate that the incorporation of the self-assembling peptide, FmocFF, in sodium alginate leads to the production of a rigid, yet injectable, hydrogel without the addition of cross-linking agents. Scanning electron microscopy reveals a nanofibrous structure which mimics the natural bone extracellular matrix. The formed composite hydrogel exhibits thixotropic behavior and a high storage modulus of approximately 10 kPA, as observed in rheological measurements. The in vitro biocompatibility tests carried out with MC3T3-E1 preosteoblast cells demonstrate good cell viability and adhesion to the hydrogel fibers. This composite scaffold can induce osteogenic differentiation and facilitate calcium mineralization, as shown by Alizarin red staining, alkaline phosphatase activity and RT-PCR analysis. The high biocompatibility, excellent mechanical properties and similarity to the native extracellular matrix suggest the utilization of this hydrogel as a temporary three-dimensional cellular microenvironment promoting bone regeneration.

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