Presence of anthraquinone in coffee and tea samples. An improved methodology based on mass spectrometry and a pilot monitoring programme

2021 ◽  
Vol 13 (1) ◽  
pp. 99-109
Author(s):  
Francisco José Díaz-Galiano ◽  
Maria Murcia-Morales ◽  
María del Mar Gómez-Ramos ◽  
Carmen Ferrer ◽  
Amadeo R. Fernández-Alba
Keyword(s):  
Mass Spectrometry ◽  
Monitoring Programme ◽  
Matrix Interferences

The developed method prevents the occurrence of matrix interferences in the analysis of anthraquinone in coffee and tea.

Supercritical Fluid Extraction and Gas Chromatography/Ion Trap Mass Spectrometry of Pentachloromtrobenzene Pesticides in Vegetables

1995 ◽  
Vol 78 (2) ◽  
pp. 445-452 ◽  
Author(s):  
Steven J Lehotay ◽  
Medina A Ibrahim
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Ion Trap ◽  
Ion Trap Mass Spectrometry ◽  
Fluid Extraction ◽  
Green Beans ◽  
Matrix Interferences ◽  
Vegetable Sample

Abstract An analytical approach using supercritical fluid extraction (SFE) followed by gas chromatography/ion trap mass spectrometry (GC/ITMS) was developed for the analysis of the fungicide pentachloromtrobenzene and several analogues in vegetables. The method was tested in the analysis of carrots, potatoes, green beans, celery, and radishes fortified with pentachloronitrobenzene, tetrachloronitrobenzene, pentachloroanisole, pentachlorothioanisole, pentachlorobenzene, hexachlorobenzene, and pentachloroaniline. An incurred carrot sample analyzed by the method was shown to contain hexachlorobenzene at 7 ± 3 ng/g, which agreed with the concentration (8 ± 4 ng/g) determined using a traditional solvent-based method. The SFE method consisted of the following steps: (1) homogenizing a 50 g vegetable sample and weighing a 3 g subsample; (2) mixing 2 g sorbent (Hydromatrix) with the subsample to absorb moisture and packing a 10 mL extraction vessel; (3) extracting with 40 mL C02 at 200 atm, 40°C, and a flow rate of 3 mL/min; and (4) collecting the extract on a 1 g alumina basic trap at 25°C and flushing with 8 mL isooctane. Collection of the extract on alumina efficiently removed chlorophyll and other matrix interferences. GC/ITMS in the electron-impact mode confirmed and quantitated the analytes at concentrations as low as 1 ng/g.

scholarly journals Quantitative mass spectrometry of unconventional human biological matrices

2016 ◽  
Vol 374 (2079) ◽  
pp. 20150380 ◽  
Author(s):  
Ewelina P. Dutkiewicz ◽  
Pawel L. Urban
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Chromatographic Separation ◽  
Certified Reference Materials ◽  
Mass Spectrometric ◽  
Quantitative Mass Spectrometry ◽  
Biological Matrices ◽  
Matrix Interferences ◽  
Non Invasive ◽  
Potential Biomarkers

The development of sensitive and versatile mass spectrometric methodology has fuelled interest in the analysis of metabolites and drugs in unconventional biological specimens. Here, we discuss the analysis of eight human matrices—hair, nail, breath, saliva, tears, meibum, nasal mucus and skin excretions (including sweat)—by mass spectrometry (MS). The use of such specimens brings a number of advantages, the most important being non-invasive sampling, the limited risk of adulteration and the ability to obtain information that complements blood and urine tests. The most often studied matrices are hair, breath and saliva. This review primarily focuses on endogenous (e.g. potential biomarkers, hormones) and exogenous (e.g. drugs, environmental contaminants) small molecules. The majority of analytical methods used chromatographic separation prior to MS; however, such a hyphenated methodology greatly limits analytical throughput. On the other hand, the mass spectrometric methods that exclude chromatographic separation are fast but suffer from matrix interferences. To enable development of quantitative assays for unconventional matrices, it is desirable to standardize the protocols for the analysis of each specimen and create appropriate certified reference materials. Overcoming these challenges will make analysis of unconventional human biological matrices more common in a clinical setting. This article is part of the themed issue ‘Quantitative mass spectrometry’.

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