Simultaneous separation of artesunate and mefloquine in fixed-dose combination tablets by CZE-UV

2020 ◽  
Vol 12 (47) ◽  
pp. 5709-5717
Author(s):  
Jéssica Cordeiro Queiroz de Souza ◽  
Paula Rocha Chellini ◽  
Alessandra Lifsitch Viçosa ◽  
Marcus Vinícius Nora de Souza ◽  
Marcone Augusto Leal de Oliveira
Keyword(s):  
Indirect Detection ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Simultaneous Separation ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Dose Combination ◽  
Novel Method ◽  
Direct And Indirect Detection

A novel method was proposed for simultaneous determination of artesunate (ATS) and mefloquine (MFQ) in fixed-dose combination tablets by capillary zone electrophoresis with simultaneous direct and indirect detection by ultraviolet (CZE-UV).

Sub-minute determination of rifampicin and isoniazid in fixed dose combination tablets by capillary zone electrophoresis with ultraviolet absorption detection

2018 ◽  
Vol 41 (24) ◽  
pp. 4533-4543 ◽  
Author(s):  
Lucas Mattos Duarte ◽  
Tatiane Lima Amorim ◽  
Paula Rocha Chellini ◽  
Luiz Henrique Cantarino Adriano ◽  
Marcone Augusto Leal de Oliveira
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Ultraviolet Absorption ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Dose Combination

Development and Validation of an HPLC Method for Simultaneous Determination of Rifampicin, Isoniazid, Pyrazinamide, and Ethambutol Hydrochloride in Pharmaceutical Formulations

2015 ◽  
Vol 98 (5) ◽  
pp. 1234-1239 ◽  
Author(s):  
Paula R Chellini ◽  
Eduardo B Lages ◽  
Pedro H C Franco ◽  
Fernando H A Nogueira ◽  
Isabela C César ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Combined Treatment ◽  
Pharmaceutical Formulations ◽  
Gradient Elution ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Array Detector ◽  
Equilibration Time ◽  
Dose Combination

Abstract Tuberculosis treatment consists of a fixed dose combination of rifampicin (RIF), isoniazid (INH), pyrazinamide (PYZ), and ethambutol hydrochloride (EMB). The combined treatment using various drugs is necessary for patient curing, without recrudescence, and for prevention of drug-resistant mutants, which may occur during treatment. An HPLC-diode array detector (DAD) method for the simultaneous determination of RIF, INH, PYZ, and EMB in fixed dose combination tablets was developed and validated. Chromatographic experiments were performed on an Agilent 1200 HPLC system, and the separation was carried out on a Purospher STAR RP18e (250 × 4.6 mm id, 5 μm, Merck) analytical column. Gradient elution was carried out with a mobile phase of 20 mM monobasic sodium phosphate buffer with 0.2% triethylamine (pH 7.0) and acetonitrile at a flow rate of 1.5 mL/min. The total run time was 12 min, and the re-equilibration time was 5 min. EMB detection was performed at 210 nm, and RIF, INH, and PYZ were detected at 238 nm, using a DAD. The method proved to be specific, linear (r2 > 0.99), precise (RSD <2%), accurate, and robust and may be applied to the QC analysis of pharmaceutical formulations.

NOVEL UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF SITAGLIPTIN PHOSPHATE AND SIMVASTATIN BY AREA UNDER CURVE METHOD

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (04) ◽  
pp. 46-52
Author(s):  
V. V. Chopde ◽  
◽  
P. M. Patil ◽  
S. D Rathod ◽  
P. D. Chaudhari
Keyword(s):  
Simultaneous Determination ◽  
Wavelength Range ◽  
Spectrophotometric Method ◽  
Area Under Curve ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Economic Method ◽  
Curve Method ◽  
Dose Combination

A simple, versatile, accurate, precise and economic method for simultaneous determination of sitagliptin phosphate and simvastatin in fixed dose combination products was developed. The absorbance values at 267 nm and 239 nm for sitagliptin phosphate and simvastatin. The combination is also estimated by AUC method it involved measurement of area under curve in the wavelength range is 264-270 nm (λ1 -λ2 ) and 236-242 nm (λ3 -λ4 ) sitagliptin phosphate and simvastatin respectively. This method obeyed Beer’s law in the concentration range of 10-60 μg /mL for sitagliptin phosphate and 2-12 μg /mL for simvastatin. The results of analyses have been validated statistically for linearity, accuracy, precision, LOD and LOQ of the proposed method.

scholarly journals DERIVATIVES OF THE RATIO SPECTRA FOR DETERMINATION OF ACETYLSALICYLIC ACID, ACETAMINOPHEN, AND CAFFEINE IN FIXED-DOSE COMBINATION FORMULATIONS: APPLICATION TO DISSOLUTION STUDIES

2020 ◽  
pp. 253-257
Author(s):  
JOSE RAUL MEDINA-LÓPEZ ◽  
JOSUE GIOVANI PACHECO PINEDA ◽  
PEDRO ALBERTO ROJAS GARFIAS ◽  
NICASIO CASTRO CHÁVEZ
Keyword(s):  
Drug Product ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Reference Drug ◽  
Zero Crossing ◽  
Dissolution Studies ◽  
Derivative Method ◽  
Dose Combination ◽  
Derivatives Of

Objective: To develop a ratio-derivative spectrophotometric method for the simultaneous quantification of acetilsalycilic acid (ASA), acetaminophen (ACE), and caffeine (CAF) in fixed-dose combination formulations. The proposed method was applied to the reference drug product Excedrin® in dissolution studies. Methods: The method is based on the use of the first-and second-derivatives of the ratio spectra and measurements at zero-crossing wavelengths. The dissolution profiles of ASA, ACE, and CAF were obtained following pharmacopeial conditions, USP Apparatus 2 at 100 rpm and 900 ml of water. Dissolution samples were treated with the proposed UV-derivative method and the results were compared with those obtained with a validated HPLC procedure. The dissolution efficiency was used to compare dissolution profiles (HPLC vs. UV-derivative method). Results: The method was linear in the range of 5-25 µg/ml of ASA, 2.5-20 µg/ml of ACE, and 1-8 µg/ml of CAF (R2>0.999, *P<0.05). The precision and accuracy of synthetic mixtures were within acceptable criteria (2.11-3.43% and 96.78-104.15%, respectively). Nitrocellulose filters were the best option to filter samples and stability of all drugs was adequate when standard solutions were stored at 4 °C during 24 h. No significant differences were found between dissolution profiles (*P>0.05). Conclusion: The proposed UV-derivative method allows the simultaneous determination of ASA, ACE, and CAF in commercial formulations. The method is simple, accurate, and precise and can be used in dissolution studies. Spectrophotometric methods are of low cost and harmless to the environment and, therefore, a better alternative than chromatographic methods.

Use of chemometrics to compare NIR and HPLC for the simultaneous determination of drug levels in fixed-dose combination tablets employed in tuberculosis treatment

2018 ◽  
Vol 149 ◽  
pp. 557-563 ◽  
Author(s):  
Kelly Sivocy Sampaio Teixeira ◽  
Said Gonçalves da Cruz Fonseca ◽  
Luís Carlos Brigido de Moura ◽  
Mario Luís Ribeiro de Moura ◽  
Márcia Herminia Pinheiro Borges ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Tuberculosis Treatment ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Drug Levels ◽  
Dose Combination

Development of a robust SFC method for evaluation of compatibility for a novel antituberculotic fixed-dose combination

2019 ◽  
Vol 11 (13) ◽  
pp. 1777-1787 ◽  
Author(s):  
Valentina Petruševska ◽  
Iva Krtalić ◽  
Andrea Rašić ◽  
Ana Mornar
Keyword(s):  
Simultaneous Determination ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Chromatography Method ◽  
Dose Combination

A fast and simple supercritical fluid chromatography method for the simultaneous determination of two antituberculotic drugs, isoniazid and rifabutin, and their impurities from a new proposed fixed-dose combination (FDC) was developed.

Sign in / Sign up

Export Citation Format

Share Document