Sub-minute determination of rifampicin and isoniazid in fixed dose combination tablets by capillary zone electrophoresis with ultraviolet absorption detection

2018 ◽  
Vol 41 (24) ◽  
pp. 4533-4543 ◽  
Author(s):  
Lucas Mattos Duarte ◽  
Tatiane Lima Amorim ◽  
Paula Rocha Chellini ◽  
Luiz Henrique Cantarino Adriano ◽  
Marcone Augusto Leal de Oliveira
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Ultraviolet Absorption ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Dose Combination

Simultaneous separation of artesunate and mefloquine in fixed-dose combination tablets by CZE-UV

2020 ◽  
Vol 12 (47) ◽  
pp. 5709-5717
Author(s):  
Jéssica Cordeiro Queiroz de Souza ◽  
Paula Rocha Chellini ◽  
Alessandra Lifsitch Viçosa ◽  
Marcus Vinícius Nora de Souza ◽  
Marcone Augusto Leal de Oliveira
Keyword(s):  
Indirect Detection ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Simultaneous Separation ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Dose Combination ◽  
Novel Method ◽  
Direct And Indirect Detection

A novel method was proposed for simultaneous determination of artesunate (ATS) and mefloquine (MFQ) in fixed-dose combination tablets by capillary zone electrophoresis with simultaneous direct and indirect detection by ultraviolet (CZE-UV).

Determination of puerarin in pharmaceutical and biological samples by capillary zone electrophoresis with UV detection

Talanta ◽  
2012 ◽  
Vol 91 ◽  
pp. 83-87 ◽  
Author(s):  
Lizhen Liu ◽  
Feng Feng ◽  
Shaomin Shuang ◽  
Yunfeng Bai ◽  
Martin M.F. Choi
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Biological Samples ◽  
Uv Detection ◽  
Zone Electrophoresis ◽  
Capillary Zone

Development and Validation of an HPLC Method for Simultaneous Determination of Rifampicin, Isoniazid, Pyrazinamide, and Ethambutol Hydrochloride in Pharmaceutical Formulations

2015 ◽  
Vol 98 (5) ◽  
pp. 1234-1239 ◽  
Author(s):  
Paula R Chellini ◽  
Eduardo B Lages ◽  
Pedro H C Franco ◽  
Fernando H A Nogueira ◽  
Isabela C César ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Combined Treatment ◽  
Pharmaceutical Formulations ◽  
Gradient Elution ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Array Detector ◽  
Equilibration Time ◽  
Dose Combination

Abstract Tuberculosis treatment consists of a fixed dose combination of rifampicin (RIF), isoniazid (INH), pyrazinamide (PYZ), and ethambutol hydrochloride (EMB). The combined treatment using various drugs is necessary for patient curing, without recrudescence, and for prevention of drug-resistant mutants, which may occur during treatment. An HPLC-diode array detector (DAD) method for the simultaneous determination of RIF, INH, PYZ, and EMB in fixed dose combination tablets was developed and validated. Chromatographic experiments were performed on an Agilent 1200 HPLC system, and the separation was carried out on a Purospher STAR RP18e (250 × 4.6 mm id, 5 μm, Merck) analytical column. Gradient elution was carried out with a mobile phase of 20 mM monobasic sodium phosphate buffer with 0.2% triethylamine (pH 7.0) and acetonitrile at a flow rate of 1.5 mL/min. The total run time was 12 min, and the re-equilibration time was 5 min. EMB detection was performed at 210 nm, and RIF, INH, and PYZ were detected at 238 nm, using a DAD. The method proved to be specific, linear (r2 > 0.99), precise (RSD <2%), accurate, and robust and may be applied to the QC analysis of pharmaceutical formulations.

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