scholarly journals An Integrated Self-Powered 3D printed Sample Concentrator for Highly Sensitive Molecular Detection of HIV in Whole Blood at the Point of Care

The Analyst ◽  
2021 ◽  
Author(s):  
Karteek Kadimisetty ◽  
Kun Yin ◽  
Aoife M. Roche ◽  
Yanjie Yi ◽  
Frederic D. Bushman ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Infectious Diseases ◽  
Whole Blood ◽  
Biological Sample ◽  
Molecular Detection ◽  
Point Of Care ◽  
Highly Sensitive ◽  
3D Printed ◽  
Self Powered

Rapid and efficient biological sample preparation and pretreatment are crucial for highly sensitive, reliable and reproducible molecular detection of infectious diseases. Herein, we report a self-powered, integrated sample concentrator (SPISC)...

scholarly journals Miniaturized devices for point of care molecular detection of HIV

Lab on a Chip ◽  
2017 ◽  
Vol 17 (3) ◽  
pp. 382-394 ◽  
Author(s):  
Michael Mauk ◽  
Jinzhao Song ◽  
Haim H. Bau ◽  
Robert Gross ◽  
Frederic D. Bushman ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Molecular Detection ◽  
Point Of Care ◽  
Isothermal Amplification ◽  
Molecular Testing ◽  
Microfluidic Technology ◽  
Technical Advances ◽  
Miniaturized Devices

We review recent technical advances in molecular testing of HIV using microfluidic technology, including sample preparation, isothermal amplification and detection.

scholarly journals 3D-printed Point-of-Care Platform for Genetic Testing of Infectious Diseases Directly in Human Samples Using Acoustic Sensors and a Smartphone

ACS Sensors ◽  
2019 ◽  
Vol 4 (5) ◽  
pp. 1329-1336 ◽  
Author(s):  
George Papadakis ◽  
Alexandros K. Pantazis ◽  
Maria Ntogka ◽  
Konstantinos Parasyris ◽  
Gesthimani-Ioanna Theodosi ◽  
...  
Keyword(s):  
Genetic Testing ◽  
Infectious Diseases ◽  
Point Of Care ◽  
Acoustic Sensors ◽  
Human Samples ◽  
3D Printed

CONTROL OF RESIDUAL QUANTITIES OF ANTIBIOTICS IN BEE BREAD ON THE BASIS OF IMMUNO-MICROCHIPS TECHNOLOGY

2019 ◽  
Vol 1 (4) ◽  
pp. 388-393
Author(s):  
A.M. Smirnov ◽  
◽  
R.T. Klochko ◽  
S.N. Luganskiy ◽  
A.B. Sokhlikov ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Sample Preparation ◽  
Infectious Diseases ◽  
Test System ◽  
Antibiotic Residues ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Highly Sensitive ◽  
Bee Bread ◽  
Chemiluminescent Immunoassay

Unregulated use of antibiotics for the treatment of infectious diseases of bees can lead to their accumulation in beekeeping products, particularly in bee bread. The most effective methods for detecting residual amounts of antibiotics in bee bread are immuno-microchips technology, based on competitive chemiluminescent immunoassay and allowing semi-automated qualitative and quantitative analysis of a large number of samples. For determining the residual amounts of oxytetracycline in bee bread, a test system with scanning chemiluminometer of Randox evidence investigator was used. The sensitivity of the method to the antibiotic oxytetracycline was 5,0 μg/kg with a confidence of 95%; the whole analysis together with sample preparation takes 3...3,5 hours and does not require large expenditures of reagents. The method of immuno-microchips technology is highly sensitive and promising for study of bee products, particularly bee bread, for the presence of antibiotic residues.

Comparison of 2 glucose analytical methodologies in immature Kemp’s ridley sea turtles: dry chemistry of plasma versus point-of-care glucometer analysis of whole blood

2021 ◽  
pp. 104063872110018
Author(s):  
Justin R. Perrault ◽  
Michael D. Arendt ◽  
Jeffrey A. Schwenter ◽  
Julia L. Byrd ◽  
Kathryn A. Tuxbury ◽  
...  
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Sea Turtles ◽  
Sample Volume ◽  
Specific Reference ◽  
Volume Data ◽  
Analytical Methodology ◽  
Glucose Determination ◽  
Kemp's Ridley Sea Turtles ◽  
Kemp's Ridley

Blood glucose measurements provide important diagnostic information regarding stress, disease, and nutritional status. Glucose analytical methodologies include dry chemistry analysis (DCA) of plasma and point-of-care (POC) glucometer analysis of whole blood; however, these 2 methods differ in cost, required sample volume, and processing time. Because POC glucometers use built-in equations based on features of mammalian blood to convert whole blood measurements to plasma equivalent units, obtained glucose data must be compared and validated using gold-standard chemistry analytical methodology in reptiles. For in-water, trawl-captured, immature Kemp’s ridley sea turtles ( Lepidochelys kempii) from Georgia, USA, we observed significant, positive agreement between the 2 glucose determination methods; however, the glucometer overestimated glucose concentrations by 1.4 mmol/L on average in comparison to DCA and produced a wider range of results. The discordance of these results suggests that POC glucometer glucose data should be interpreted in the context of methodology- and brand-specific reference intervals along with concurrent packed cell volume data.

scholarly journals PERFORMANCE CHARACTERISTICS OF A FRET-BASED IMMUNOASSAY FOR QUANTITATION OF INFLIXIMAB ON A POINT-OF-CARE INSTRUMENT SYSTEM

2021 ◽  
Vol 27 (Supplement_1) ◽  
pp. S57-S57
Author(s):  
Edgar Ong ◽  
Ruo Huang ◽  
Richard Kirkland ◽  
Michael Hale ◽  
Larry Mimms
Keyword(s):  
Whole Blood ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Quantitative Detection ◽  
Therapeutic Drug ◽  
Analytical Sensitivity ◽  
Sample Type ◽  
Hook Effect ◽  
Limit Of Quantitation ◽  
Assay Precision

Abstract Introduction A fast (<5 min), time-resolved fluorescence resonance energy transfer (FRET)-based immunoassay was developed for the quantitative detection of infliximab (IFX) and biosimilars for use in therapeutic drug monitoring using only 20 µL of fingerstick whole blood or serum at the point-of-care. The Procise IFX assay and ProciseDx analyzer are CE-marked. Studies were performed to characterize analytical performance of the Procise IFX assay on the ProciseDx analyzer. Methods Analytical testing was performed by spiking known amounts of IFX into negative serum and whole blood specimens. Analytical sensitivity was determined using limiting concentrations of IFX. Linearity was determined by testing IFX across the assay range. Hook effect was assessed at IFX concentrations beyond levels expected to be found within a patient. Testing of assay precision, cross-reactivity and potential interfering substances, and biosimilars was performed. The Procise IFX assay was also compared head-to-head with another CE-marked assay: LISA-TRACKER infliximab ELISA test (Theradiag, France). The accuracy of the Procise IFX assay is established through calibrators and controls traceable to the WHO 1st International Standard for Infliximab (NIBSC code: 16/170). Results The Procise IFX assay shows a Limit of Blank, Limit of Detection, and Lower Limit of Quantitation (LLoQ) of 0.1, 0.2, and 1.1 µg/mL in serum and 0.6, 1.1, and 1.7 µg/mL in whole blood, respectively. The linear assay range was determined to be 1.7 to 77.2 µg/mL in serum and whole blood. No hook effect was observed at an IFX concentration of 200 µg/mL as the value reported as “>ULoQ”. Assay precision testing across 20 days with multiple runs and reagent lots showed an intra-assay coefficient of variation (CV) of 2.7%, an inter-assay CV of <2%, and a total CV of 3.4%. The presence of potentially interfering/cross-reacting substances showed minimal impact on assay specificity with %bias within ±8% of control. Testing of biosimilars (infliximab-dyyb and infliximab-abda) showed good recovery. A good correlation to the Theradiag infliximab ELISA was obtained for both serum (slope=1.01; r=0.99) and whole blood (slope=1.01; r=0.98) samples (Figure 1). Conclusion Results indicate that the Procise IFX assay is sensitive, specific, and precise yielding results within 5 minutes from both whole blood and serum without the operator needing to specify sample type. Additionally, it shows very good correlation to a comparator assay that takes several hours and sample manipulation to yield results. This makes the Procise IFX assay ideal for obtaining fast and accurate IFX quantitation, thus allowing for immediate drug level dosing decisions to be made by the physician during patient treatment.

3D Printed Smart Silk Wearable Sensors

The Analyst ◽  
2021 ◽  
Author(s):  
Tianshu Chu ◽  
Huili Wang ◽  
Yumeng Qiu ◽  
Haoxi Luo ◽  
Bingfang He ◽  
...  
Keyword(s):  
Point Of Care ◽  
Wearable Sensors ◽  
Point Of Care Testing ◽  
Biochemical Sensing ◽  
3D Printed ◽  
Physiological And Biochemical

Wearable sensors play a key role in point-of-care testing (POCT) for its flexible and integration capability on sensitive physiological and biochemical sensing. Here, we present a multifunction wearable silk patch...

Highly sensitive and stable fructose self-powered biosensor based on a self-charging biosupercapacitor

2021 ◽  
Vol 176 ◽  
pp. 112909
Author(s):  
Paolo Bollella ◽  
Zhanna Boeva ◽  
Rose-Marie Latonen ◽  
Kenji Kano ◽  
Lo Gorton ◽  
...  
Keyword(s):  
Highly Sensitive ◽  
Self Powered

scholarly journals Turntable Paper-Based Device to Detect Escherichia coli

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 194
Author(s):  
Yung-Chih Wang ◽  
Yao-Hung Tsai ◽  
Ching-Fen Shen ◽  
Ming-Yao He ◽  
Yi-Chen Fu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Infectious Diseases ◽  
Point Of Care ◽  
Multiple Integral ◽  
Enzyme Linked Immunosorbent Assay ◽  
Considerable Time ◽  
E Coli ◽  
Colony Forming Unit ◽  
Experimental Implementation ◽  
Colony Forming Units

Escherichia coli has been known to cause a variety of infectious diseases. The conventional enzyme-linked immunosorbent assay (ELISA) is a well-known method widely used to diagnose a variety of infectious diseases. This method is expensive and requires considerable time and effort to conduct and complete multiple integral steps. We previously proposed the use of paper-based ELISA to rapidly detect the presence of E. coli. This approach has demonstrated utility for point-of-care (POC) urinary tract infection diagnoses. Paper-based ELISA, while advantageous, still requires the execution of several procedural steps. Here, we discuss the design and experimental implementation of a turntable paper-based device to simplify the paper-based ELISA protocols for the detection of E. coli. In this process, antibodies or reagents are preloaded onto zones of a paper-based device and allowed to dry before use. We successfully used this device to detect E. coli with a detection limit of 105 colony-forming units (colony-forming unit [CFU])/mL.

3D printed stretchable smart fibers and textiles for self-powered e-skin

Nano Energy ◽  
2021 ◽  
Vol 84 ◽  
pp. 105866 ◽  
Author(s):  
Yuxin Chen ◽  
Zhirong Deng ◽  
Ri Ouyang ◽  
Renhao Zheng ◽  
Zhiqiang Jiang ◽  
...  
Keyword(s):  
3D Printed ◽  
Self Powered
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