A DNA-functionalized biomass nanoprobe for the targeted photodynamic therapy of tumor and ratiometric fluorescence imaging-based visual cancer cell identification/antitumor drug screening

The Analyst ◽  
2021 ◽  
Author(s):  
Pengxiang Lin ◽  
Liangliang Zhang ◽  
Dongxia Chen ◽  
Jiayao Xu ◽  
Yulong Bai ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Cancer Cell ◽  
Drug Screening ◽  
Antitumor Drug ◽  
Ratiometric Fluorescence ◽  
Cell Identification ◽  
Targeted Photodynamic Therapy

A DNA-functionalized biomass nanoprobe was developed for the targeted photodynamic therapy of tumor and ratiometric fluorescence imaging-based visual cancer cell identification/antitumor drug screening.

Ratiometric fluorescence imaging of endogenous selenocysteine in cancer cell matrix

2017 ◽  
Vol 5 (33) ◽  
pp. 6890-6896 ◽  
Author(s):  
Yong Tian ◽  
Fangyun Xin ◽  
Congcong Gao ◽  
Jing Jing ◽  
Xiaoling Zhang
Keyword(s):  
Fluorescence Imaging ◽  
Cancer Cell ◽  
Blue Emission ◽  
Yellow Emission ◽  
Fluorescence Ratio ◽  
Ratiometric Fluorescence ◽  
Specific Reaction ◽  
Quantitative Correlation ◽  
Cell Matrix ◽  
A Cell

A ratiometric method for tracing the variation of selenocysteine (Sec) in a cell matrix was provided. This was constructed by the quantitative correlation between the fluorescence ratio ofRat-Sec(blue emission, the probe) andNap-OH(green-yellow emission, the product from a Sec-specific reaction) and the concentration of Sec.

Cancer cell membrane-coated biomimetic platform for tumor targeted photodynamic therapy and hypoxia-amplified bioreductive therapy

Biomaterials ◽  
2017 ◽  
Vol 142 ◽  
pp. 149-161 ◽  
Author(s):  
Shi-Ying Li ◽  
Hong Cheng ◽  
Wen-Xiu Qiu ◽  
Lu Zhang ◽  
Shuang-Shuang Wan ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cell Membrane ◽  
Cancer Cell ◽  
Targeted Photodynamic Therapy

Enhanced cancer therapy by the marriage of metabolic alteration and mitochondrial-targeted photodynamic therapy using cyclometalated Ir(iii) complexes

2017 ◽  
Vol 53 (71) ◽  
pp. 9878-9881 ◽  
Author(s):  
Jiangping Liu ◽  
Chengzhi Jin ◽  
Bo Yuan ◽  
Yu Chen ◽  
Xingguo Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Therapy ◽  
Cancer Cell ◽  
Tumor Spheroids ◽  
Two Photon ◽  
Metabolic Alteration ◽  
Targeted Photodynamic Therapy

A series of DCA-Ir(iii) co-drug complexes were demonstrated to act in synergy by sensitizing cancer cell for PDT to achieve cancer-specifically enhanced two-photon PDT in the hypoxic muticellular tumor spheroids.

scholarly journals A Dual-Fluorophore Sensor Approach for Ratiometric Fluorescence Imaging of Potassium in Living Cells

2020 ◽  
Author(s):  
Zeming Wang ◽  
Tyler C. Detomasi ◽  
Christopher Chang
Keyword(s):  
Fluorescence Imaging ◽  
Cell Lines ◽  
Cancer Cell ◽  
High Selectivity ◽  
Cancer Cell Lines ◽  
Biological Evaluation ◽  
The Body ◽  
Ratiometric Fluorescence ◽  
Intracellular Potassium ◽  
Ratiometric Detection

Potassium is the most abundant intracellular metal in the body, playing vital roles in regulating intracellular fluid volume, nutrient transport, and cell-to-cell communication through nerve and muscle contraction. On the other hand, aberrant alterations in K<sup>+</sup> homeostasis contribute to a diverse array of diseases spanning cardiovascular and neurological disorders to diabetes to kidney disease to cancer. Owing to the large differences in intracellular versus extracellular K<sup>+</sup> concentrations ([K<sup>+</sup>]<sub>intra</sub> = 150 mM, [K<sup>+</sup>]<sub>extra</sub> = 3-5 mM), an unmet need for studies of K<sup>+</sup> physiology and pathology remains a relative dearth of methods to reliably measure dynamic changes in intracellular K<sup>+</sup> in biological specimens that meet the dual challenges of low affinity and high selectivity for K<sup>+</sup>, particularly over Na<sup>+</sup>, as currently available fluorescent K<sup>+</sup> sensors are largely optimized with high-affinity receptors that are more amenable for extracellular K<sup>+</sup> detection. We report the design, synthesis, and biological evaluation of Ratiometric Potassium Sensor 1 (<b>RPS-1</b>), a dual-fluorophore sensor that enables ratiometric fluorescence imaging of intracellular potassium in living systems. <b>RPS-1</b> links a potassium-responsive fluorescent sensor fragment (<b>PS525</b>) with a low-affinity, high-selectivity crown ether receptor for K<sup>+</sup> to a potassium-insensitive reference fluorophore (<b>Coumarin 343</b>) as an internal calibration standard through ester bonds. Upon intracellular delivery, esterase-directed cleavage splits these two dyes into separate fragments to enable ratiometric detection of K<sup>+</sup>. <b>RPS-1</b> responds to K<sup>+</sup> in aqueous buffer with high selectivity over competing metal ions and is sensitive to potassium ions at steady-state intracellular levels and can respond to decreases or increases from that basal set point. Moreover, <b>RPS-1</b> was applied for comparative screening of K<sup>+</sup> pools across a panel of different cancer cell lines, revealing elevations in basal intracellular K<sup>+</sup> in metastatic breast cancer cell lines vs normal breast cells. This work provides a unique chemical tool for the study of intracellular potassium dynamics and a starting point for the design of other ratiometric fluorescent sensors based on two-fluorophore approaches that do not rely on FRET or related energy transfer designs.

Dual functionalized natural biomass carbon dots from lychee exocarp for cancer cell targetable near-infrared fluorescence imaging and photodynamic therapy

Nanoscale ◽  
2018 ◽  
Vol 10 (38) ◽  
pp. 18124-18130 ◽  
Author(s):  
Mingyue Xue ◽  
Jingjin Zhao ◽  
Zhihua Zhan ◽  
Shulin Zhao ◽  
Chuanqing Lan ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Cancer Cell ◽  
Carbon Dots ◽  
Near Infrared ◽  
Biomass Carbon ◽  
Near Infrared Fluorescence ◽  
Near Infrared Fluorescence Imaging

Dual functionalized natural biomass carbon dots from lychee exocarp were fabricated for cancer cell targetable near-infrared fluorescence imaging and photodynamic therapy.

scholarly journals A multifunctional nanoprobe for targeting tumors and mitochondria with singlet oxygen generation and monitoring mitochondrion pH changes in cancer cells by ratiometric fluorescence imaging

2020 ◽  
Vol 11 (14) ◽  
pp. 3636-3643 ◽  
Author(s):  
Jingjin Zhao ◽  
Mengbing Zou ◽  
Mengjiao Huang ◽  
Liangliang Zhang ◽  
Keqin Yang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Ratiometric Fluorescence ◽  
Oxygen Generation ◽  
Ph Changes ◽  
Singlet Oxygen Generation ◽  
Multifunctional Nanoprobe

A multifunctional nanoprobe with singlet oxygen generation that can target tumors and mitochondria was developed for photodynamic therapy of tumors and monitoring mitochondrion pH changes in cancer cells by ratiometric fluorescence imaging.

ATP-Activatable Photosensitizer Enables Dual Fluorescence Imaging and Targeted Photodynamic Therapy of Tumor

2017 ◽  
Vol 89 (24) ◽  
pp. 13610-13617 ◽  
Author(s):  
Yizhong Shen ◽  
Qian Tian ◽  
Yidan Sun ◽  
Jing-Juan Xu ◽  
Deju Ye ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Dual Fluorescence ◽  
Targeted Photodynamic Therapy

scholarly journals A Dual-Fluorophore Sensor Approach for Ratiometric Fluorescence Imaging of Potassium in Living Cells

2020 ◽  
Author(s):  
Zeming Wang ◽  
Tyler C. Detomasi ◽  
Christopher Chang
Keyword(s):  
Fluorescence Imaging ◽  
Cell Lines ◽  
Cancer Cell ◽  
High Selectivity ◽  
Cancer Cell Lines ◽  
Biological Evaluation ◽  
The Body ◽  
Ratiometric Fluorescence ◽  
Intracellular Potassium ◽  
Ratiometric Detection

Potassium is the most abundant intracellular metal in the body, playing vital roles in regulating intracellular fluid volume, nutrient transport, and cell-to-cell communication through nerve and muscle contraction. On the other hand, aberrant alterations in K<sup>+</sup> homeostasis contribute to a diverse array of diseases spanning cardiovascular and neurological disorders to diabetes to kidney disease to cancer. Owing to the large differences in intracellular versus extracellular K<sup>+</sup> concentrations ([K<sup>+</sup>]<sub>intra</sub> = 150 mM, [K<sup>+</sup>]<sub>extra</sub> = 3-5 mM), an unmet need for studies of K<sup>+</sup> physiology and pathology remains a relative dearth of methods to reliably measure dynamic changes in intracellular K<sup>+</sup> in biological specimens that meet the dual challenges of low affinity and high selectivity for K<sup>+</sup>, particularly over Na<sup>+</sup>, as currently available fluorescent K<sup>+</sup> sensors are largely optimized with high-affinity receptors that are more amenable for extracellular K<sup>+</sup> detection. We report the design, synthesis, and biological evaluation of Ratiometric Potassium Sensor 1 (<b>RPS-1</b>), a dual-fluorophore sensor that enables ratiometric fluorescence imaging of intracellular potassium in living systems. <b>RPS-1</b> links a potassium-responsive fluorescent sensor fragment (<b>PS525</b>) with a low-affinity, high-selectivity crown ether receptor for K<sup>+</sup> to a potassium-insensitive reference fluorophore (<b>Coumarin 343</b>) as an internal calibration standard through ester bonds. Upon intracellular delivery, esterase-directed cleavage splits these two dyes into separate fragments to enable ratiometric detection of K<sup>+</sup>. <b>RPS-1</b> responds to K<sup>+</sup> in aqueous buffer with high selectivity over competing metal ions and is sensitive to potassium ions at steady-state intracellular levels and can respond to decreases or increases from that basal set point. Moreover, <b>RPS-1</b> was applied for comparative screening of K<sup>+</sup> pools across a panel of different cancer cell lines, revealing elevations in basal intracellular K<sup>+</sup> in metastatic breast cancer cell lines vs normal breast cells. This work provides a unique chemical tool for the study of intracellular potassium dynamics and a starting point for the design of other ratiometric fluorescent sensors based on two-fluorophore approaches that do not rely on FRET or related energy transfer designs.

scholarly journals Fine-tuning the electronic structure of heavy-atom-free BODIPY photosensitizers for fluorescence imaging and mitochondria-targeted photodynamic therapy

2020 ◽  
Vol 11 (25) ◽  
pp. 6479-6484 ◽  
Author(s):  
Sujie Qi ◽  
Nahyun Kwon ◽  
Yubin Yim ◽  
Van-Nghia Nguyen ◽  
Juyoung Yoon
Keyword(s):  
Electronic Structure ◽  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Fluorescence Imaging ◽  
Heavy Atom ◽  
Fine Tuning ◽  
Oxygen Generation ◽  
Singlet Oxygen Generation ◽  
Targeted Photodynamic Therapy ◽  
Mitochondria Targeting

We designed and investigated novel mitochondria-targeting heavy-atom-free BODIPY photosensitizers (R-BODs) that possessed considerable singlet oxygen generation abilities and good fluorescence properties for imaging-guided photodynamic therapy (PDT).

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