Rapid and sensitive electrochemical detection of microRNAs by gold nanoparticle-catalyzed silver enhancement

The Analyst ◽  
2020 ◽  
Vol 145 (24) ◽  
pp. 7893-7897 ◽  
Author(s):  
Qilin Wen ◽  
Xiaolin Liang ◽  
Hongcheng Pan ◽  
Jianping Li ◽  
Yun Zhang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Gold Nanoparticle ◽  
Electrochemical Detection ◽  
Silver Enhancement

Using the proposed method the microRNA assay was successfully carried out in less than 70 min and the detection limit was as low as 15 fM.

scholarly journals Optical Readout of Gold Nanoparticle-Based DNA Microarrays without Silver Enhancement

2006 ◽  
Vol 90 (1) ◽  
pp. L13-L15 ◽  
Author(s):  
Gerhard A. Blab ◽  
Laurent Cognet ◽  
Stéphane Berciaud ◽  
Isabelle Alexandre ◽  
Dieter Husar ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Dna Microarrays ◽  
Silver Enhancement ◽  
Optical Readout

scholarly journals Femtomolar and selective dopamine detection by a gold nanoparticle enhanced surface plasmon resonance aptasensor

2018 ◽  
Author(s):  
Yong Cao ◽  
Mark T. McDermott
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cdna Probe ◽  
Dna Aptamer ◽  
Dopamine Detection ◽  
Calibration Curves ◽  
20 Nm

ABSTRACTUltrasensitive and selective detection and quantification of dopamine (DA) plays a key role in monitoring neurodegenerative diseases. However, the detection limit reported for DA detection is typically in the lower nM range. Pushing the detection limit to pM or lower for this particular target to cover the physiological levels (< 130 pM) is significant. Herein, DA DNA aptamer (DAAPT) gold nanoparticle (AuNP) conjugate is utilized to enhance the surface plasmon resonance (SPR) signal, which enables to detect and quantify DA in the femtomolar (200 fM) to picomolar range. To the best of our knowledge, this is the lowest detection limit achieved for SPR sensing of dopamine. The as-prepared 10 nm DAAPT-AuNP conjugate demonstrates strong binding affinity (Kd = 3.1 ± 1.4 nM) to the complementary DNA (cDNA) probe on gold chip. The cDNA probe is immobilized to the chip via polydopamine surface chemistry, which allows the Michael addition of any primary amine-terminated biomolecules. By adjusting the concentration of the DAAPT-AuNP conjugate, two calibration curves are generated with dynamic ranges from 100 µM to 2 mM, and from 200 fM to 20 nM, respectively. Both calibration curves have negative slopes, showing good agreement to a dose-response curve in an enzyme inhibition assay. In addition, the sensing strategy is evaluated to be specific for DA detection using a series of DA analogs and other metabolites as potential interferences.

A multiple-promoted silver enhancement strategy in electrochemical detection of target virus

2014 ◽  
Vol 194 ◽  
pp. 276-282 ◽  
Author(s):  
Xindong Wang ◽  
Laijun Wang ◽  
Wei Yang ◽  
Shiyun Ai
Keyword(s):  
Electrochemical Detection ◽  
Silver Enhancement

scholarly journals Electrochemical detection of plant virus using gold nanoparticle-modified electrodes

2019 ◽  
Vol 1046 ◽  
pp. 123-131 ◽  
Author(s):  
Mohga Khater ◽  
Alfredo de la Escosura-Muñiz ◽  
Daniel Quesada-González ◽  
Arben Merkoçi
Keyword(s):  
Gold Nanoparticle ◽  
Electrochemical Detection ◽  
Plant Virus ◽  
Modified Electrodes

Electrochemical Detection of Tributyrin using Gold Nanoparticle Functionalized Screen-Printed Electrode

2021 ◽  
Author(s):  
A. Aidil ◽  
S. R. Balakrishnan ◽  
C.W. Zanariah ◽  
W.Z. Wan Ismail ◽  
M. Sahrim ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Electrochemical Detection ◽  
Screen Printed Electrode ◽  
Screen Printed

Gold nanoparticle/DNA-based nanobioconjugate for electrochemical detection of Zika virus

2020 ◽  
Vol 187 (11) ◽  
Author(s):  
Sebastian Cajigas ◽  
Daniel Alzate ◽  
Jahir Orozco
Keyword(s):  
Gold Nanoparticle ◽  
Electrochemical Detection ◽  
Zika Virus

Elaborately manufacturing an electrochemical aptasensor based on gold nanoparticle/COF composites for amplified detection performance

2020 ◽  
Vol 8 (47) ◽  
pp. 16984-16991
Author(s):  
Qian-Qian Zhu ◽  
Wen-Wen Zhang ◽  
Han-Wen Zhang ◽  
Rongrong Yuan ◽  
Hongming He
Keyword(s):  
Gold Nanoparticle ◽  
Electrochemical Detection ◽  
Detection Performance ◽  
Electrochemical Aptasensor

An electrochemical aptasensor based on Au@COF is designed and fabricated with excellent electrochemical detection performance.

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