scholarly journals Assessment of human plasma and urine sample preparation for reproducible and high-throughput UHPLC-MS clinical metabolic phenotyping

The Analyst ◽  
2020 ◽  
Vol 145 (20) ◽  
pp. 6511-6523 ◽  
Author(s):  
Andrew D. Southam ◽  
Liam D. Haglington ◽  
Lukáš Najdekr ◽  
Andris Jankevics ◽  
Ralf J. M. Weber ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Urine Sample ◽  
High Throughput ◽  
Human Urine ◽  
Metabolic Phenotyping ◽  
Preparation Methods ◽  
Human Plasma And Urine ◽  
Multiple Sample ◽  
Sample Preparation Methods

In this study we assess multiple sample preparation methods for UHPLC-MS metabolic phenotyping analysis of human urine and plasma. All methods are discussed in terms of metabolite and lipid coverage and reproducibility.

Determination of Glimepiride in Human Plasma by LC-MS-MS and Comparison of Sample Preparation Methods for Glimepiride

2004 ◽  
Vol 60 (1-2) ◽  
Author(s):  
Hohyun Kim ◽  
Kyu Young Chang ◽  
Chang Hun Park ◽  
Moon Sun Jang ◽  
Jung-Ae Lee ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Preparation Methods ◽  
Sample Preparation Methods

Evaluation of horse urine sample preparation methods for metabolomics using LC coupled to HRMS

Bioanalysis ◽  
2014 ◽  
Vol 6 (6) ◽  
pp. 785-803 ◽  
Author(s):  
Natali Stojiljkovic ◽  
Alain Paris ◽  
Patrice Garcia ◽  
Marie-Agnès Popot ◽  
Yves Bonnaire ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Urine Sample ◽  
Preparation Methods ◽  
Sample Preparation Methods

Comparison of Two Sample Preparation Methods for a HPLC-DAD Assay of Tolbutamide from Human Plasma

2000 ◽  
Vol 33 (11) ◽  
pp. 2219-2230 ◽  
Author(s):  
A. Medvedovici ◽  
V. David ◽  
D. Miron ◽  
C. Mircioiu
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Preparation Methods ◽  
Sample Preparation Methods

scholarly journals Optimization of small-scale sample preparation for high-throughput OpenArray analysis

2021 ◽  
Vol 8 (1) ◽  
pp. e143
Author(s):  
Neeta A. Abraham ◽  
Anne C. Campbell ◽  
Warren D. Hirst ◽  
Catherine L. Nezich
Keyword(s):  
Gene Expression ◽  
Sample Preparation ◽  
High Throughput ◽  
Dynamic Range ◽  
Cultured Cells ◽  
Starting Material ◽  
Small Scale ◽  
Preparation Methods ◽  
Single Well ◽  
Sample Preparation Methods

OpenArray is one of the most high-throughput qPCR platforms available but its efficiency can be limited by sample preparation methods that are slow and costly. To optimize the sample workflow for high-throughput qPCR processing by OpenArray, small-scale sample preparation methods were compared for compatibility with this system to build confidence in a method that maintains quality and accuracy while using less starting material and saving time and money. This study is the first to show that the Cells-to-CT kit can be used to prepare samples within the dynamic range of OpenArray directly from cultured cells in a single well of a 96-well plate when used together with a cDNA preamplification PCR step. Use of Cells-to-CT produced results of similar quality and accuracy to that of a preparation method using purified RNA in less than half the sample preparation time. While Cells-to-CT samples also exhibited slightly increased variance, which affects the ability of OpenArray to distinguish small differences in gene expression, overall gene expression mean results correlated well between small-scale methods. This work demonstrates that Cells-to-CT with preamplification can be used to reliably prepare samples for OpenArray analysis while saving time, money, and starting material.

Improving Sample Preparation Methods For Cannabis-Infused Edible And Topical Products

Planta Medica ◽  
2016 ◽  
Vol 82 (05) ◽  
Author(s):  
M Wilcox ◽  
M Jacyno ◽  
J Marcu ◽  
J Neal-Kababick
Keyword(s):  
Sample Preparation ◽  
Preparation Methods ◽  
Sample Preparation Methods
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