HPLC-MS/MS analysis of anthocyanins in human plasma and urine using protein precipitation and dilute-and-shoot sample preparation methods, respectively

2018 ◽  
Vol 32 (5) ◽  
pp. e4177 ◽  
Author(s):  
Junguo Liu ◽  
Jiuxue Song ◽  
Karen Huang ◽  
Deborah Michel ◽  
Jim Fang
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Protein Precipitation ◽  
Preparation Methods ◽  
Ms Analysis ◽  
Human Plasma And Urine ◽  
Sample Preparation Methods

scholarly journals Assessment of human plasma and urine sample preparation for reproducible and high-throughput UHPLC-MS clinical metabolic phenotyping

The Analyst ◽  
2020 ◽  
Vol 145 (20) ◽  
pp. 6511-6523 ◽  
Author(s):  
Andrew D. Southam ◽  
Liam D. Haglington ◽  
Lukáš Najdekr ◽  
Andris Jankevics ◽  
Ralf J. M. Weber ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Urine Sample ◽  
High Throughput ◽  
Human Urine ◽  
Metabolic Phenotyping ◽  
Preparation Methods ◽  
Human Plasma And Urine ◽  
Multiple Sample ◽  
Sample Preparation Methods

In this study we assess multiple sample preparation methods for UHPLC-MS metabolic phenotyping analysis of human urine and plasma. All methods are discussed in terms of metabolite and lipid coverage and reproducibility.

Determination of Glimepiride in Human Plasma by LC-MS-MS and Comparison of Sample Preparation Methods for Glimepiride

2004 ◽  
Vol 60 (1-2) ◽  
Author(s):  
Hohyun Kim ◽  
Kyu Young Chang ◽  
Chang Hun Park ◽  
Moon Sun Jang ◽  
Jung-Ae Lee ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Preparation Methods ◽  
Sample Preparation Methods

Comparison of Two Sample Preparation Methods for a HPLC-DAD Assay of Tolbutamide from Human Plasma

2000 ◽  
Vol 33 (11) ◽  
pp. 2219-2230 ◽  
Author(s):  
A. Medvedovici ◽  
V. David ◽  
D. Miron ◽  
C. Mircioiu
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Preparation Methods ◽  
Sample Preparation Methods

Matrix interference in LC-ESI-MS/MS analysis of metanephrines in protein precipitated plasma samples

2019 ◽  
Vol 26 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Riin Rebane ◽  
Koit Herodes
Keyword(s):  
Sample Preparation ◽  
Blood Plasma ◽  
Matrix Effects ◽  
Protein Precipitation ◽  
Plasma Samples ◽  
Internal Standards ◽  
Preparation Methods ◽  
Esi Ms ◽  
Sample Preparation Methods ◽  
In Blood Plasma

Metanephrine and normetanephrine are measured in blood plasma to diagnose different diseases. Simpler sample preparation procedures are preferred but tend to yield less purified extracts. Therefore, thorough investigation of matrix effects is required. In this work, several sample preparation methods and chromatographic modes were compared for liquid chromatography tandem mass spectrometric (with electrospray ionization; LC-ESI-MS/MS) analysis of metanephrine and normetanephrine in blood plasma. Protein precipitation with methanol was found to be sufficient for sample preparation and pentafluorophenyl column provided adequate chromatographic separation. A new cheaper and less labor-intensive approach is proposed where necessary quantitation limits are achieved through a sample preparation containing only protein precipitation and dilution of the sample extract. Matrix effects for different sample preparation methods and the use of isotope-labeled internal standards were evaluated. Unusual interference to D3-labeled internal standard of normetanephrine was discovered – signal of interfering compound increased while the matrix effects were reduced by dilution, e.g. dilution eliminates matrix suppression on interfering compound. The results stress the need to monitor interfering compounds and evaluate matrix effects at every step of method development. Matrix effects and interferences can be different for analytes and their corresponding isotopically labeled internal standards. This means that the use of isotopically labeled internal standards cannot guarantee accuracy of obtained results. New method allows quantification of the low nanomolar concentrations of metanephrine and normetanephrine in plasma samples.

Improving Sample Preparation Methods For Cannabis-Infused Edible And Topical Products

Planta Medica ◽  
2016 ◽  
Vol 82 (05) ◽  
Author(s):  
M Wilcox ◽  
M Jacyno ◽  
J Marcu ◽  
J Neal-Kababick
Keyword(s):  
Sample Preparation ◽  
Preparation Methods ◽  
Sample Preparation Methods
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