Sensitive detection of intracellular telomerase activity via double signal amplification and ratiometric fluorescence resonance energy transfer

The Analyst ◽  
2020 ◽  
Vol 145 (21) ◽  
pp. 6992-6999
Author(s):  
Bei Zhang ◽  
Lu Shi ◽  
Wei Liu ◽  
Baoxin Li ◽  
Yan Jin

A reliable, sensitive and immobilization-free method was developed for the detection of intracellular telomerase activity by combining double signal amplification with ratiometric FRET.

2017 ◽  
Vol 53 (80) ◽  
pp. 11016-11019 ◽  
Author(s):  
Li-Juan Wang ◽  
Ming-Li Luo ◽  
Qianyi Zhang ◽  
Bo Tang ◽  
Chun-Yang Zhang

We developed a single quantum dot-based fluorescence resonance energy transfer nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase.


The Analyst ◽  
2016 ◽  
Vol 141 (21) ◽  
pp. 6133-6139 ◽  
Author(s):  
Yanjun Wang ◽  
Luzhu Yang ◽  
Baoxin Li ◽  
Yan Jin

A homogeneous and PCR-free fluorescent strategy has been developed for sensitively and specifically studying telomerase activity and inhibition via GNR-based fluorescence resonance energy transfer.


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