Sensitive detection of telomerase activity in cells using a DNA-based fluorescence resonance energy transfer nanoprobe

2020 ◽  
Vol 1098 ◽  
pp. 133-139 ◽  
Author(s):  
Guohai Yang ◽  
Qian Zhang ◽  
Lin Ma ◽  
Youwei Zheng ◽  
Fei Tian ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Telomerase Activity ◽  
Sensitive Detection ◽  
Fluorescence Resonance ◽  
In Cells

Sensitive detection of intracellular telomerase activity via double signal amplification and ratiometric fluorescence resonance energy transfer

The Analyst ◽  
2020 ◽  
Vol 145 (21) ◽  
pp. 6992-6999
Author(s):  
Bei Zhang ◽  
Lu Shi ◽  
Wei Liu ◽  
Baoxin Li ◽  
Yan Jin
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Signal Amplification ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Telomerase Activity ◽  
Sensitive Detection ◽  
Ratiometric Fluorescence ◽  
Fluorescence Resonance

A reliable, sensitive and immobilization-free method was developed for the detection of intracellular telomerase activity by combining double signal amplification with ratiometric FRET.

Single quantum dot-based nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase

2017 ◽  
Vol 53 (80) ◽  
pp. 11016-11019 ◽  
Author(s):  
Li-Juan Wang ◽  
Ming-Li Luo ◽  
Qianyi Zhang ◽  
Bo Tang ◽  
Chun-Yang Zhang
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Sensitive Detection ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Single Quantum ◽  
Single Quantum Dot ◽  
Fluorescence Resonance

We developed a single quantum dot-based fluorescence resonance energy transfer nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase.

Monitoring the intracellular transformation process of surface-cleavable PLGA particles containing disulfide bonds by fluorescence resonance energy transfer

2015 ◽  
Vol 3 (45) ◽  
pp. 8865-8873 ◽  
Author(s):  
Dahai Yu ◽  
Guangyang Zou ◽  
Xiaojing Cui ◽  
Zhengwei Mao ◽  
Irina Estrela-Lopis ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Disulfide Bonds ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Transformation Process ◽  
Fluorescence Resonance ◽  
Kinetics Of ◽  
Surface Cleavage ◽  
In Cells

The FRET technique was used to quantify the surface cleavage kinetics of PLGA particles containing disulfide bonds in cells.

Homogeneous and ultrasensitive detection of telomerase activity via gold nanorod-based fluorescence resonance energy transfer

The Analyst ◽  
2016 ◽  
Vol 141 (21) ◽  
pp. 6133-6139 ◽  
Author(s):  
Yanjun Wang ◽  
Luzhu Yang ◽  
Baoxin Li ◽  
Yan Jin
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Telomerase Activity ◽  
Gold Nanorod ◽  
Ultrasensitive Detection ◽  
Fluorescence Resonance

A homogeneous and PCR-free fluorescent strategy has been developed for sensitively and specifically studying telomerase activity and inhibition via GNR-based fluorescence resonance energy transfer.

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