scholarly journals Development of an inexpensive Raman-compatible substrate for the construction of a microarray screening platform

The Analyst ◽  
2020 ◽  
Vol 145 (21) ◽  
pp. 7030-7039
Author(s):  
Isamar Pastrana-Otero ◽  
Sayani Majumdar ◽  
Aidan E. Gilchrist ◽  
Brittney L. Gorman ◽  
Brendan A. C. Harley ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Raman Spectroscopy ◽  
Stem Cell ◽  
Single Cell ◽  
Cell Fate ◽  
Engineering Applications ◽  
Stem Cell Fate ◽  
Cell Fate Decisions ◽  
Microarray Screening ◽  
Screening Platform

Development of an inexpensive substrate that is compatible with the chemistries used to fabricate biomaterial microarrays for tissue engineering applications and noninvasive single-cell Raman spectroscopy for identifying stem cell fate decisions.

scholarly journals Single-cell analyses to reveal hematopoietic stem cell fate decisions

FEBS Letters ◽  
2017 ◽  
Vol 591 (15) ◽  
pp. 2195-2212 ◽  
Author(s):  
Ilaria Lunger ◽  
Malak Fawaz ◽  
Michael A. Rieger
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Cell Fate ◽  
Hematopoietic Stem Cell ◽  
Hematopoietic Stem ◽  
Stem Cell Fate ◽  
Cell Fate Decisions

High-throughput stem cell-based phenotypic screening through microniches

2019 ◽  
Vol 7 (8) ◽  
pp. 3471-3479
Author(s):  
Laura Kolb ◽  
Simone Allazetta ◽  
Maria Karlsson ◽  
Mehmet Girgin ◽  
Wilfried Weber ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Cell Fate ◽  
High Throughput ◽  
Cell Behavior ◽  
Phenotypic Screening ◽  
Stem Cell Fate ◽  
Screening Platform

Methods for screening combinations of signals for their effects on stem cell behavior are needed in the field of tissue engineering. We introduce a microgel-based screening platform for testing combinations of proteins on stem cell fate.

scholarly journals Polyamine-controlled proliferation and protein biosynthesis are independent determinants of hair follicle stem cell fate

2020 ◽  
Author(s):  
Kira Allmeroth ◽  
Christine S. Kim ◽  
Andrea Annibal ◽  
Andromachi Pouikli ◽  
Carlos Andrés Chacón-Martínez ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hair Follicle ◽  
Cell Fate ◽  
Protein Biosynthesis ◽  
Mrna Translation ◽  
Stem Cell Differentiation ◽  
List Type ◽  
Stem Cell Fate ◽  
Cell Fate Decisions ◽  
Hair Follicle Stem Cell

AbstractStem cell differentiation is accompanied by an increase in mRNA translation. The rate of protein biosynthesis is influenced by the polyamines putrescine, spermidine, and spermine that are essential for cell growth and stem cell maintenance. However, the role of polyamines as endogenous effectors of stem cell fate and whether they act through translational control remains obscure. Here, we investigated the function of polyamines in stem cell fate decisions using hair follicle stem cell (HFSC) organoids. HFSCs showed lower translation rates than progenitor cells, and a forced suppression of translation by direct targeting of the ribosome or through specific depletion of natural polyamines elevated stemness. In addition, we identified N1-acetylspermidine as a novel parallel regulator of cell fate decisions, increasing proliferation without reducing translation. Overall, this study delineates the diverse routes of polyamine metabolism-mediated regulation of stem cell fate decisions.Key PointsLow mRNA translation rates characterize hair follicle stem cell (HFSC) stateDepletion of natural polyamines enriches HFSCs via reduced translationN1-acetylspermidine promotes HFSC state without reducing translationN1-acetylspermidine expands the stem cell pool through elevated proliferation

Histone crotonylation regulates neural stem cell fate decisions by activating bivalent promoters

EMBO Reports ◽  
2021 ◽  
Author(s):  
Shang‐Kun Dai ◽  
Pei‐Pei Liu ◽  
Hong‐Zhen Du ◽  
Xiao Liu ◽  
Ya‐Jie Xu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Neural Stem Cell ◽  
Stem Cell Fate ◽  
Cell Fate Decisions
Sign in / Sign up

Export Citation Format

Share Document