High-throughput stem cell-based phenotypic screening through microniches

2019 ◽  
Vol 7 (8) ◽  
pp. 3471-3479
Author(s):  
Laura Kolb ◽  
Simone Allazetta ◽  
Maria Karlsson ◽  
Mehmet Girgin ◽  
Wilfried Weber ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Cell Fate ◽  
High Throughput ◽  
Cell Behavior ◽  
Phenotypic Screening ◽  
Stem Cell Fate ◽  
Screening Platform

Methods for screening combinations of signals for their effects on stem cell behavior are needed in the field of tissue engineering. We introduce a microgel-based screening platform for testing combinations of proteins on stem cell fate.

scholarly journals Development of an inexpensive Raman-compatible substrate for the construction of a microarray screening platform

The Analyst ◽  
2020 ◽  
Vol 145 (21) ◽  
pp. 7030-7039
Author(s):  
Isamar Pastrana-Otero ◽  
Sayani Majumdar ◽  
Aidan E. Gilchrist ◽  
Brittney L. Gorman ◽  
Brendan A. C. Harley ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Raman Spectroscopy ◽  
Stem Cell ◽  
Single Cell ◽  
Cell Fate ◽  
Engineering Applications ◽  
Stem Cell Fate ◽  
Cell Fate Decisions ◽  
Microarray Screening ◽  
Screening Platform

Development of an inexpensive substrate that is compatible with the chemistries used to fabricate biomaterial microarrays for tissue engineering applications and noninvasive single-cell Raman spectroscopy for identifying stem cell fate decisions.

scholarly journals Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

2019 ◽  
Vol 2019 ◽  
pp. 1-16 ◽  
Author(s):  
Fei Xing ◽  
Lang Li ◽  
Changchun Zhou ◽  
Cheng Long ◽  
Lina Wu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Growth Factors ◽  
Cell Fate ◽  
Chemical Cues ◽  
Physical Factors ◽  
Stem Cell Fate ◽  
Physical And Chemical

It is well known that stem cells reside within tissue engineering functional microenvironments that physically localize them and direct their stem cell fate. Recent efforts in the development of more complex and engineered scaffold technologies, together with new understanding of stem cell behavior in vitro, have provided a new impetus to study regulation and directing stem cell fate. A variety of tissue engineering technologies have been developed to regulate the fate of stem cells. Traditional methods to change the fate of stem cells are adding growth factors or some signaling pathways. In recent years, many studies have revealed that the geometrical microenvironment played an essential role in regulating the fate of stem cells, and the physical factors of scaffolds including mechanical properties, pore sizes, porosity, surface stiffness, three-dimensional structures, and mechanical stimulation may affect the fate of stem cells. Chemical factors such as cell-adhesive ligands and exogenous growth factors would also regulate the fate of stem cells. Understanding how these physical and chemical cues affect the fate of stem cells is essential for building more complex and controlled scaffolds for directing stem cell fate.

Artificial niche microarrays for probing single stem cell fate in high throughput

2011 ◽  
Vol 8 (11) ◽  
pp. 949-955 ◽  
Author(s):  
Samy Gobaa ◽  
Sylke Hoehnel ◽  
Marta Roccio ◽  
Andrea Negro ◽  
Stefan Kobel ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
High Throughput ◽  
Stem Cell Fate

scholarly journals Hydrogels Containing Gradients in Vascular Density Reveal Dose-Dependent Role of Angiocrine Cues on Stem Cell Behavior

2021 ◽  
Author(s):  
Mai T. Ngo ◽  
Victoria R. Barnhouse ◽  
Aidan E. Gilchrist ◽  
Christine J. Hunter ◽  
Joy N. Hensold ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Three Dimensional ◽  
Cell Behavior ◽  
Vascular Density ◽  
Microfluidic Mixing ◽  
Hematopoietic Stem ◽  
Stem Cell Fate ◽  
Signaling Axis ◽  
Dose Dependent

AbstractBiomaterials that replicate patterns of microenvironmental signals from the stem cell niche offer the potential to refine platforms to regulate stem cell behavior. While significant emphasis has been placed on understanding the effects of biophysical and biochemical cues on stem cell fate, vascular-derived or angiocrine cues offer an important alternative signaling axis for biomaterial-based stem cell platforms. Elucidating dose-dependent relationships between angiocrine cues and stem cell fate are largely intractable in animal models and two-dimensional cell culture. In this study, we leverage microfluidic mixing devices to generate three-dimensional hydrogels containing lateral gradients in vascular density alongside murine hematopoietic stem cells (HSCs). Regional differences in vascular density can be generated via embossed gradients in cell, matrix, or growth factor density. HSCs co-cultured alongside vascular gradients reveal spatial patterns of HSC phenotype in response to angiocrine signals. Notably, decreased Akt signaling in high vessel density regions led to increased expansion of lineage-positive hematopoietic cells. This approach offers a combinatorial tool to rapidly screen a continuum of microenvironments with varying vascular, biophysical, and biochemical cues to reveal the influence of local angiocrine signals on HSC fate.

Sign in / Sign up

Export Citation Format

Share Document