Surface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers via immuno-capture LI-MS

The Analyst ◽  
2020 ◽  
Vol 145 (19) ◽  
pp. 6237-6242
Author(s):  
Toby Siu-Chung Tam ◽  
Yu-Hong Cheng ◽  
Chun-Nam Lok ◽  
Ho Yu Au-Yeung ◽  
Wen-Xiu Ni ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Surface Functionalization ◽  
Protein Detection ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Specific Interactions ◽  
Protein Biomarkers ◽  
Optimal Surface ◽  
Surface Optimization

Optimal surface functionalization of antibody-conjugated AuNPs for modulating specific versus non-specific interactions to enhance protein detection sensitivity.

scholarly journals Microneedle-based skin patch for blood-free rapid diagnostic testing

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Xue Jiang ◽  
Peter B. Lillehoj
Keyword(s):  
Interstitial Fluid ◽  
Point Of Care ◽  
Diagnostic Testing ◽  
Protein Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Detection Sensitivity ◽  
Sample Collection ◽  
Protein Biomarkers ◽  
Skin Patch

Abstract Rapid diagnostic tests are one of the most commonly used tests to detect and screen for infectious diseases in the developing world. While these tests are simple, inexpensive, and readily available, they rely on finger-prick blood sampling, which requires trained medical personnel, poses risks of infection, and can complicate cooperation in young children, asymptomatic individuals, and communities with blood taboos. Here, we report a novel microneedle-based skin patch for the rapid detection of protein biomarkers in dermal interstitial fluid. Sample collection is facilitated by a hydrophilic hollow microneedle array that autonomously extracts and transports interstitial fluid to an antibody-based lateral flow test strip via surface tension for colorimetric antigen detection. We employ a simple gold enhancement treatment to enhance the detection sensitivity of this colloidal gold-based lateral flow assay and elucidate the underlying mechanism of this enhancement mechanism through experimental investigation. For proof-of-concept, this device was used to detect Plasmodium falciparum histidine-rich protein 2, a biomarker for malaria infection, which could be detected at concentrations as low as 8 ng/mL. Each test can be completed in <20 min and requires no equipment. To the best of our knowledge, this work is the first demonstration of a microneedle-based lateral flow assay for rapid protein detection in dermal interstitial fluid. In addition to its simplicity, minimally invasive nature, and low cost, this diagnostic device can be readily adapted to detect other protein biomarkers in interstitial fluid, making it a promising tool for point-of-care testing.

Monodispersity of magnetic immuno-nanoprobes enhances the detection sensitivity of low abundance biomarkers in one drop of serum

The Analyst ◽  
2015 ◽  
Vol 140 (22) ◽  
pp. 7678-7686 ◽  
Author(s):  
Rey Y. Capangpangan ◽  
Mira Anne C. dela Rosa ◽  
Rofeamor P. Obena ◽  
Yu-Jen Chou ◽  
Der-Lii Tzou ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Protein Biomarkers

A streamlined protocol for biocompatible monodisperse magnetic nanoparticles for sensitive detection of low abundance protein biomarkers.

scholarly journals A Proteomic Study on the Personalized Protein Corona of Liposomes. Relevance for Early Diagnosis of Pancreatic DUCTAL Adenocarcinoma and Biomarker Detection

2021 ◽  
Vol 2 (2) ◽  
pp. 82-93
Author(s):  
Luca Digiacomo ◽  
Francesca Giulimondi ◽  
Daniela Pozzi ◽  
Alessandro Coppola ◽  
Vincenzo La Vaccara ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Early Diagnosis ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Protein Corona ◽  
Detection Sensitivity ◽  
Ductal Adenocarcinoma ◽  
Protein Biomarkers ◽  
Ca 19.9 ◽  
Proteomic Study

Due to late diagnosis, high incidence of metastasis, and poor survival rate, pancreatic cancer is one of the most leading cause of cancer-related death. Although manifold recent efforts have been done to achieve an early diagnosis of pancreatic cancer, CA-19.9 is currently the unique biomarker that is adopted for the detection, despite its limits in terms of sensitivity and specificity. To identify potential protein biomarkers for pancreatic ductal adenocarcinoma (PDAC), we used three model liposomes as nanoplatforms that accumulate proteins from human plasma and studied the composition of this biomolecular layer, which is known as protein corona. Indeed, plasma proteins adsorb on nanoparticle surface according to their abundance and affinity to the employed nanomaterial, thus even small differences between healthy and PDAC protein expression levels can be, in principle, detected. By mass spectrometry experiments, we quantified such differences and identified possible biomarkers for PDAC. Some of them are already known to exhibit different expressions in PDAC proteomes, whereas the role of other relevant proteins is still not clear. Therefore, we predict that the employment of nanomaterials and their protein corona may represent a useful tool to amplify the detection sensitivity of cancer biomarkers, which may be used for the early diagnosis of PDAC, with clinical implication for the subsequent therapy in the context of personalized medicine.

scholarly journals Electrokinetic Formation of “Microbridges” for Protein Biomarkers as Sensors

2007 ◽  
Vol 12 (5) ◽  
pp. 311-317 ◽  
Author(s):  
Vindhya Kunduru ◽  
Shalini Prasad
Keyword(s):  
Electric Fields ◽  
Microelectrode Array ◽  
Protein Detection ◽  
High Specificity ◽  
Detection Methods ◽  
Label Free ◽  
Protein Biomarkers ◽  
Detection Scheme ◽  
Polystyrene Beads ◽  
Conducting Path

We demonstrate a technique to detect protein biomarkers contained in vulnerable coronary plaque using a platform-based microelectrode array (MEA). The detection scheme is based on the property of high specificity binding between antibody and antigen similar to most immunoassay techniques. Rapid clinical diagnosis can be achieved by detecting the amount of protein in blood by analyzing the protein's electrical signature. Polystyrene beads which act as transportation agents for the immobile proteins (antigen) are electrically aligned by application of homogenous electric fields. The principle of electrophoresis is used to produce calculated electrokinetic movement among the anti-C-reactive protein (CRP), or in other words antibody funtionalized polystyrene beads. The electrophoretic movement of antibody-functionalized polystyrene beads results in the formation of “Microbridges” between the two electrodes of interest which aid in the amplification of the antigen—antibody binding event. Sensitive electrical equipment is used for capturing the amplified signal from the “Microbridge” which essentially behaves as a conducting path between the two electrodes. The technique circumvents the disadvantages of conventional protein detection methods by being rapid, noninvasive, label-free, repeatable, and inexpensive. The same principle of detection can be applied for any receptor—ligand-based system because the technique is based only on the volume of the analyte of interest. Detection of the inflammatory coronary disease biomarker CRP is achieved at concentration levels spanning over the lower microgram/milliliter to higher order nanogram/milliliter ranges.

A target-responsive autonomous aptamer machine biosensor for enzyme-free and sensitive detection of protein biomarkers

2018 ◽  
Vol 6 (24) ◽  
pp. 4146-4150 ◽  
Author(s):  
Mengqi Zou ◽  
Daxiu Li ◽  
Ruo Yuan ◽  
Yun Xiang
Keyword(s):  
Sensitive Detection ◽  
Protein Biomarkers ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Target-triggered operation of an aptamer machine leads to amplified and highly sensitive detection of protein biomarkers.

Highly sensitive and rapid detection of citrus Huanglongbing pathogen (Candidatus Liberibacter asiaticus) using Cas12a-based methods

2021 ◽  
Author(s):  
Matthew Wheatley ◽  
Yong-Ping Duan ◽  
Yinong Yang
Keyword(s):  
Early Detection ◽  
Nucleic Acids ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Candidatus Liberibacter Asiaticus ◽  
Fluorescent Reporter ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus

Citrus Huanglongbing (HLB) or greening is one of the most devastating diseases of citrus worldwide. Sensitive detection of its causal agent, Candidatus Liberibacter asiaticus (CLas), is critical for early diagnosis and successful management of HLB. However, current nucleic acid-based detection methods are often insufficient for the early detection of CLas from asymptomatic tissue, and unsuitable for high-throughput and field-deployable diagnosis of HLB. Here we report the development of the Cas12a-based DETECTR (DNA endonuclease-targeted CRISPR trans reporter) assay for highly specific and sensitive detection of CLas nucleic acids from infected samples. The DETECTR assay, which targets the five-copy nrdB gene specific to CLas, couples isothermal amplification with Cas12a trans-cleavage of fluorescent reporter oligos and enables detection of CLas nucleic acids at the attomolar level. The DETECTR assay was capable of specifically detecting the presence of CLas across different infected citrus, periwinkle and psyllid samples, and shown to be compatible with lateral flow assay technology for potential field-deployable diagnosis. The improvements in detection sensitivity and flexibility of the DETECTR technology position the assay as a potentially suitable tool for early detection of CLas in infected regions.

scholarly journals Sensitive Detection of H2S Using Gold Nanoparticle Decorated Single-Walled Carbon Nanotubes

2010 ◽  
Vol 82 (1) ◽  
pp. 250-257 ◽  
Author(s):  
Syed Mubeen ◽  
Ting Zhang ◽  
Nicha Chartuprayoon ◽  
Youngwoo Rheem ◽  
Ashok Mulchandani ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Gold Nanoparticle ◽  
Single Walled Carbon Nanotubes ◽  
Sensitive Detection ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

scholarly journals All-Metal Terahertz Metamaterial Biosensor for Protein Detection

2021 ◽  
Author(s):  
Gangqi Wang ◽  
Fengjie Zhu ◽  
Tingting Lang ◽  
Jianjun Liu ◽  
Zhi Hong ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Refractive Index ◽  
Bovine Serum Albumin ◽  
Figure Of Merit ◽  
Limit Of Detection ◽  
Protein Detection ◽  
Cost Effective ◽  
Detection Sensitivity ◽  
Drilling Technology ◽  
Simulation Results

Abstract In this paper, a terahertz (THz) biosensor based on all-metal metamaterial is theoretically investigated and experimentally verified. This THz metamaterial biosensor uses stainless steel materials that are manufactured via laser-drilling technology. The simulation results show that the maximum refractive index (RI) sensitivity and the figure of merit (FOM) of this metamaterial sensor are 294.95 GHz/RIU and 4.03, respectively. Then, bovine serum albumin (BSA) was chosen as the detection substance to assess this biosensor’s effectiveness. The experiment results show that the detection sensitivity is 72.81 GHz/(ng/mm2) and the limit of detection (LOD) is 0.035 mg/mL. This THz metamaterial biosensor is simple, cost-effective, easy to fabricate, and have great potential in various biosensing applications.

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