Monodispersity of magnetic immuno-nanoprobes enhances the detection sensitivity of low abundance biomarkers in one drop of serum

The Analyst ◽  
2015 ◽  
Vol 140 (22) ◽  
pp. 7678-7686 ◽  
Author(s):  
Rey Y. Capangpangan ◽  
Mira Anne C. dela Rosa ◽  
Rofeamor P. Obena ◽  
Yu-Jen Chou ◽  
Der-Lii Tzou ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Protein Biomarkers

A streamlined protocol for biocompatible monodisperse magnetic nanoparticles for sensitive detection of low abundance protein biomarkers.

Surface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers via immuno-capture LI-MS

The Analyst ◽  
2020 ◽  
Vol 145 (19) ◽  
pp. 6237-6242
Author(s):  
Toby Siu-Chung Tam ◽  
Yu-Hong Cheng ◽  
Chun-Nam Lok ◽  
Ho Yu Au-Yeung ◽  
Wen-Xiu Ni ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Surface Functionalization ◽  
Protein Detection ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Specific Interactions ◽  
Protein Biomarkers ◽  
Optimal Surface ◽  
Surface Optimization

Optimal surface functionalization of antibody-conjugated AuNPs for modulating specific versus non-specific interactions to enhance protein detection sensitivity.

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

scholarly journals A Proteomic Study on the Personalized Protein Corona of Liposomes. Relevance for Early Diagnosis of Pancreatic DUCTAL Adenocarcinoma and Biomarker Detection

2021 ◽  
Vol 2 (2) ◽  
pp. 82-93
Author(s):  
Luca Digiacomo ◽  
Francesca Giulimondi ◽  
Daniela Pozzi ◽  
Alessandro Coppola ◽  
Vincenzo La Vaccara ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Early Diagnosis ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Protein Corona ◽  
Detection Sensitivity ◽  
Ductal Adenocarcinoma ◽  
Protein Biomarkers ◽  
Ca 19.9 ◽  
Proteomic Study

Due to late diagnosis, high incidence of metastasis, and poor survival rate, pancreatic cancer is one of the most leading cause of cancer-related death. Although manifold recent efforts have been done to achieve an early diagnosis of pancreatic cancer, CA-19.9 is currently the unique biomarker that is adopted for the detection, despite its limits in terms of sensitivity and specificity. To identify potential protein biomarkers for pancreatic ductal adenocarcinoma (PDAC), we used three model liposomes as nanoplatforms that accumulate proteins from human plasma and studied the composition of this biomolecular layer, which is known as protein corona. Indeed, plasma proteins adsorb on nanoparticle surface according to their abundance and affinity to the employed nanomaterial, thus even small differences between healthy and PDAC protein expression levels can be, in principle, detected. By mass spectrometry experiments, we quantified such differences and identified possible biomarkers for PDAC. Some of them are already known to exhibit different expressions in PDAC proteomes, whereas the role of other relevant proteins is still not clear. Therefore, we predict that the employment of nanomaterials and their protein corona may represent a useful tool to amplify the detection sensitivity of cancer biomarkers, which may be used for the early diagnosis of PDAC, with clinical implication for the subsequent therapy in the context of personalized medicine.

A target-responsive autonomous aptamer machine biosensor for enzyme-free and sensitive detection of protein biomarkers

2018 ◽  
Vol 6 (24) ◽  
pp. 4146-4150 ◽  
Author(s):  
Mengqi Zou ◽  
Daxiu Li ◽  
Ruo Yuan ◽  
Yun Xiang
Keyword(s):  
Sensitive Detection ◽  
Protein Biomarkers ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Target-triggered operation of an aptamer machine leads to amplified and highly sensitive detection of protein biomarkers.

Highly sensitive and rapid detection of citrus Huanglongbing pathogen (Candidatus Liberibacter asiaticus) using Cas12a-based methods

2021 ◽  
Author(s):  
Matthew Wheatley ◽  
Yong-Ping Duan ◽  
Yinong Yang
Keyword(s):  
Early Detection ◽  
Nucleic Acids ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Candidatus Liberibacter Asiaticus ◽  
Fluorescent Reporter ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus

Citrus Huanglongbing (HLB) or greening is one of the most devastating diseases of citrus worldwide. Sensitive detection of its causal agent, Candidatus Liberibacter asiaticus (CLas), is critical for early diagnosis and successful management of HLB. However, current nucleic acid-based detection methods are often insufficient for the early detection of CLas from asymptomatic tissue, and unsuitable for high-throughput and field-deployable diagnosis of HLB. Here we report the development of the Cas12a-based DETECTR (DNA endonuclease-targeted CRISPR trans reporter) assay for highly specific and sensitive detection of CLas nucleic acids from infected samples. The DETECTR assay, which targets the five-copy nrdB gene specific to CLas, couples isothermal amplification with Cas12a trans-cleavage of fluorescent reporter oligos and enables detection of CLas nucleic acids at the attomolar level. The DETECTR assay was capable of specifically detecting the presence of CLas across different infected citrus, periwinkle and psyllid samples, and shown to be compatible with lateral flow assay technology for potential field-deployable diagnosis. The improvements in detection sensitivity and flexibility of the DETECTR technology position the assay as a potentially suitable tool for early detection of CLas in infected regions.

Low fouling and ultrasensitive electrochemical immunosensors with dual assay methods based on Fe3O4 magnetic nanoparticles

2019 ◽  
Vol 7 (38) ◽  
pp. 5842-5847 ◽  
Author(s):  
Wenshi Li ◽  
Gao-Chao Fan ◽  
Xiaojian Fan ◽  
Ruiqiao Zhang ◽  
Lei Wang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Analytical Methods ◽  
Sensitive Detection ◽  
Fe3o4 Magnetic Nanoparticles ◽  
Cancer Antigen ◽  
Electrochemical Immunosensors ◽  
Highly Sensitive ◽  
Assay Methods ◽  
Highly Sensitive Detection

Low fouling electrochemical immunosensors for the highly sensitive detection of cancer antigen 15-3 with both “signal-off” and “signal-on” analytical methods were developed.

Luminescent magnetic nanoparticles encapsulated in MOFs for highly selective and sensitive detection of ClO−/SCN− and anti-counterfeiting

Nanoscale ◽  
2018 ◽  
Vol 10 (18) ◽  
pp. 8667-8676 ◽  
Author(s):  
Chaorui Li ◽  
Jun Hai ◽  
Siliang Li ◽  
Baodui Wang ◽  
Zhengyin Yang
Keyword(s):  
Magnetic Nanoparticles ◽  
Fluorescent Probe ◽  
Pure Water ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Zeolitic Imidazolate Framework ◽  
Framework Materials ◽  
Ultra Pure Water

Luminous Eu(iii) complex-functionalized Fe3O4 nanoparticles encapsulated into zeolitic imidazolate framework materials (nano-ZIF-8) were prepared and employed as a fluorescent probe for detecting ClO− and SCN− with good selectivity and high sensitivity in ultra-pure water.

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