Single-particle spectroscopy and defocused imaging of anisotropic gold nanorods by total internal reflection scattering microscopy

The Analyst ◽  
2020 ◽  
Vol 145 (18) ◽  
pp. 6038-6044 ◽  
Author(s):  
Jaeran Lee ◽  
Geun Wan Kim ◽  
Ji Won Ha
Keyword(s):  
Gold Nanorods ◽  
Single Particle ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Single Particle Spectroscopy ◽  
Defocused Imaging

Total internal reflection scattering (TIRS) microscopy provided higher S/N ratio and more accurate angular information of gold nanorods.

scholarly journals Functional Characterization of an Electromagnetic Perceptive Protein

2020 ◽  
Author(s):  
Sunayana Mitra ◽  
Carlo Barnaba ◽  
Jens Schmidt ◽  
Galit Pelled ◽  
Assaf A. Gilad
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Total Internal Reflection ◽  
Magnetic Stimulation ◽  
New Technologies ◽  
Cellular Function ◽  
Single Particle Tracking ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Diffusion Dynamics

AbstractMagnetoreception, the response to geomagnetic fields is a well described phenomenon in nature. However, it is likely that convergent evolution led to different mechanisms in different organisms. One intriguing example is the unique Electromagnetic Perceptive Gene (EPG) from the glass catfish Kryptopterus vitreolus, that can remotely control cellular function, upon magnetic stimulation in in-vitro and in animal models. Here, we report for the first time the cellular location and orientation of the EPG protein. We utilized a differential labelling technique in determining that the EPG protein is a membrane anchored protein with an N-terminal extracellular domain. The kinetics and diffusion dynamics of the EPG protein in response to magnetic stimulation was also elucidated using single particle imaging and tracking. Pulse chase labelling and Total Internal Reflection Fluorescence (TIRF) imaging revealed an increase in EPG kinetics post magnetic stimulation activation at a single particle level. Trajectory analysis show notably different EPG protein kinetics before and after magnetic stimulation in both 2 (free vs bound particle) and 3 state (free vs intermediate vs bound particle) tracking models. These data serve to provide additional information that support and understand the underlying biophysical mechanisms behind EPG activation by magnetic stimulation. In conclusion, our results provide evidence for the basis of magnetoreception in EPG protein that would aid in future studies that seek to understand this novel mechanism. This study is important for understanding the phenomenon of magnetoreception as well as developing new technologies for magnetogenetics – the utilization of electromagnetic fields to remotely control cellular function.Graphical TOCElucidation of magnetoreception in a fish derived Electromagnetic Perceptive Gene (EPG), using genetic tagging and single particle tracking with Total Internal Reflection Fluorescence (TIRF) suggests changes in kinetics of membranal motion upon stimulation by magnetic field.

scholarly journals Single-particle evanescent light scattering simulations for total internal reflection microscopy

2006 ◽  
Vol 45 (28) ◽  
pp. 7299 ◽  
Author(s):  
Laurent Helden ◽  
Elena Eremina ◽  
Norbert Riefler ◽  
Christopher Hertlein ◽  
Clemens Bechinger ◽  
...  
Keyword(s):  
Light Scattering ◽  
Single Particle ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Microscopy ◽  
Evanescent Light

scholarly journals Label-Free Single-Particle Imaging of the Influenza Virus by Objective-Type Total Internal Reflection Dark-Field Microscopy

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e49208 ◽  
Author(s):  
Sawako Enoki ◽  
Ryota Iino ◽  
Nobuhiro Morone ◽  
Kunihiro Kaihatsu ◽  
Shouichi Sakakihara ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Single Particle ◽  
Total Internal Reflection ◽  
Dark Field ◽  
Internal Reflection ◽  
Label Free ◽  
Dark Field Microscopy ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
Objective Type

Single-Particle Spectroscopy of Gold Nanorods beyond the Quasi-Static Limit: Varying the Width at Constant Aspect Ratio

2010 ◽  
Vol 114 (11) ◽  
pp. 4934-4938 ◽  
Author(s):  
Liane S. Slaughter ◽  
Wei-Shun Chang ◽  
Pattanawit Swanglap ◽  
Alexei Tcherniak ◽  
Bishnu P. Khanal ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
Gold Nanorods ◽  
Single Particle ◽  
Static Limit ◽  
Single Particle Spectroscopy

Comparison of sensitivity of the refractometric methods of frustrated total internal reflection and surface plasmon resonance

2020 ◽  
pp. 44-49
Author(s):  
I. N. Pavlov
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Total Internal Reflection ◽  
Resonance Method ◽  
Internal Reflection ◽  
Optical Methods ◽  
Thin Boundary Layer ◽  
Experimental Conditions ◽  
Frustrated Total Internal Reflection

Two optical methods, namely surface plasmon resonance imaging and frustrated total internal reflection, are described in the paper in terms of comparing their sensitivity to change of refractive index of a thin boundary layer of an investigated medium. It is shown that, despite the fact that the theoretically calculated sensitivity is higher for the frustrated total internal reflection method, and the fact that usually in practice the surface plasmon resonance method, on the contrary, is considered more sensitive, under the same experimental conditions both methods show a similar result.

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