scholarly journals Micromirror Total Internal Reflection Microscopy for High-Performance Single Particle Tracking at Interfaces

ACS Photonics ◽  
2021 ◽  
Author(s):  
Xuanhui Meng ◽  
Adar Sonn-Segev ◽  
Anne Schumacher ◽  
Daniel Cole ◽  
Gavin Young ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Total Internal Reflection ◽  
High Performance ◽  
Single Particle Tracking ◽  
Internal Reflection ◽  
Total Internal Reflection Microscopy

scholarly journals Functional Characterization of an Electromagnetic Perceptive Protein

2020 ◽  
Author(s):  
Sunayana Mitra ◽  
Carlo Barnaba ◽  
Jens Schmidt ◽  
Galit Pelled ◽  
Assaf A. Gilad
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Total Internal Reflection ◽  
Magnetic Stimulation ◽  
New Technologies ◽  
Cellular Function ◽  
Single Particle Tracking ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Diffusion Dynamics

AbstractMagnetoreception, the response to geomagnetic fields is a well described phenomenon in nature. However, it is likely that convergent evolution led to different mechanisms in different organisms. One intriguing example is the unique Electromagnetic Perceptive Gene (EPG) from the glass catfish Kryptopterus vitreolus, that can remotely control cellular function, upon magnetic stimulation in in-vitro and in animal models. Here, we report for the first time the cellular location and orientation of the EPG protein. We utilized a differential labelling technique in determining that the EPG protein is a membrane anchored protein with an N-terminal extracellular domain. The kinetics and diffusion dynamics of the EPG protein in response to magnetic stimulation was also elucidated using single particle imaging and tracking. Pulse chase labelling and Total Internal Reflection Fluorescence (TIRF) imaging revealed an increase in EPG kinetics post magnetic stimulation activation at a single particle level. Trajectory analysis show notably different EPG protein kinetics before and after magnetic stimulation in both 2 (free vs bound particle) and 3 state (free vs intermediate vs bound particle) tracking models. These data serve to provide additional information that support and understand the underlying biophysical mechanisms behind EPG activation by magnetic stimulation. In conclusion, our results provide evidence for the basis of magnetoreception in EPG protein that would aid in future studies that seek to understand this novel mechanism. This study is important for understanding the phenomenon of magnetoreception as well as developing new technologies for magnetogenetics – the utilization of electromagnetic fields to remotely control cellular function.Graphical TOCElucidation of magnetoreception in a fish derived Electromagnetic Perceptive Gene (EPG), using genetic tagging and single particle tracking with Total Internal Reflection Fluorescence (TIRF) suggests changes in kinetics of membranal motion upon stimulation by magnetic field.

scholarly journals High performance, LED powered, waveguide based total internal reflection microscopy

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
Srinivasan Ramachandran ◽  
Daniel A. Cohen ◽  
Arjan P. Quist ◽  
Ratnesh Lal
Keyword(s):  
Total Internal Reflection ◽  
High Performance ◽  
Internal Reflection ◽  
Total Internal Reflection Microscopy

scholarly journals Single-particle evanescent light scattering simulations for total internal reflection microscopy

2006 ◽  
Vol 45 (28) ◽  
pp. 7299 ◽  
Author(s):  
Laurent Helden ◽  
Elena Eremina ◽  
Norbert Riefler ◽  
Christopher Hertlein ◽  
Clemens Bechinger ◽  
...  
Keyword(s):  
Light Scattering ◽  
Single Particle ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Microscopy ◽  
Evanescent Light

scholarly journals Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy

Entropy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. 498
Author(s):  
Chen Zhang ◽  
Kevin Welsher
Keyword(s):  
Fisher Information ◽  
Particle Tracking ◽  
Single Particle ◽  
Biological Samples ◽  
Tracking System ◽  
Single Particle Tracking ◽  
Uniform Sampling ◽  
Single Dimension ◽  
3D Localization ◽  
Particle Localization

In this work, we present a 3D single-particle tracking system that can apply tailored sampling patterns to selectively extract photons that yield the most information for particle localization. We demonstrate that off-center sampling at locations predicted by Fisher information utilizes photons most efficiently. When performing localization in a single dimension, optimized off-center sampling patterns gave doubled precision compared to uniform sampling. A ~20% increase in precision compared to uniform sampling can be achieved when a similar off-center pattern is used in 3D localization. Here, we systematically investigated the photon efficiency of different emission patterns in a diffraction-limited system and achieved higher precision than uniform sampling. The ability to maximize information from the limited number of photons demonstrated here is critical for particle tracking applications in biological samples, where photons may be limited.

pH-Mediated nanoparticle dynamics in hydrogel nanocomposites

Soft Matter ◽  
2021 ◽  
Author(s):  
Katie A. Rose ◽  
Daeyeon Lee ◽  
Russell J. Composto
Keyword(s):  
Quantum Dot ◽  
Ethylene Glycol ◽  
Particle Tracking ◽  
Single Particle ◽  
Silica Particles ◽  
Single Particle Tracking ◽  
Poly Ethylene Glycol ◽  
Hydrogel Nanocomposites ◽  
Poly Ethylene ◽  
Nanoparticle Dynamics

The effect of static silica particles on the dynamics of quantum dot (QD) nanoparticles grafted with a poly(ethylene glycol) (PEG) brush in hydrogel nanocomposites is investigated using single particle tracking (SPT).

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