Simultaneous quantification of peroxidase and ascorbic acid in biosamples with an automatic system based on a Fe(iii)/methylthymol blue-carbon dot simulative enzyme

The Analyst ◽  
2020 ◽  
Vol 145 (16) ◽  
pp. 5438-5449 ◽  
Author(s):  
Yang Zhao ◽  
Qiao-Jing Li ◽  
Yong-Sheng Li ◽  
Xiu-Feng Gao

Simultaneous and automatic quantification of peroxidase and ascorbic acid based on one reaction system and application of a carbon dot simulative enzyme.

2018 ◽  
Vol 1 (3) ◽  
pp. 777-782 ◽  
Author(s):  
Qingqing Tan ◽  
Ruirui Zhang ◽  
Weisu Kong ◽  
Fengli Qu ◽  
Limin Lu

Talanta ◽  
2017 ◽  
Vol 162 ◽  
pp. 135-142 ◽  
Author(s):  
Tian Tian ◽  
Yaping Zhong ◽  
Chun Deng ◽  
Hao Wang ◽  
Yu He ◽  
...  

2011 ◽  
Vol 110-116 ◽  
pp. 1732-1735
Author(s):  
Jia Hong He ◽  
Qiang Xu ◽  
Zhong Rong Song ◽  
Hai Yan Kuang

A Platinum nanoparticles modified Au electrode has been successfully fabricated by using an in situ growth method. In this method, the Platinum nanoparticles could be grown on the Au electrode surface via the one-step immersion into the mixture of H2PtCl6 (analytical grade, 1g/L), NaBH4 (analytical grade) and polyvinylpyrrolidone K30 (PVP, analytical grade). A certain amount of PVP was added into the reaction system to prevent the coagulation of the Platinum nanoparticles, which obtained by the chemical redox reaction of H2PtCl6 and NaBH4. The structures and morphologies of the Platinum nanoparticles were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) . The direct electrochemical behavior of ascorbic acid in 0.3 mol/L NaCl medium at the Platinum nanoparticles modified electrode has been investigated in detail. Compared to a bare Au electrode, a substantial decrease in the overvoltage of the ascorbic acid was observed at the Platinum nanoparticles modified electrode with oxidation starting at ca. 0.20 V vs. SCE (saturated KCl). At an applied potential of 0.18V, this modified electrode produced high and reproducible sensitivity to ascorbic acid and linear responses were obtained over a concentration range from 0.600 to 3.267 μmol/L with a detection limit of 1.9 nmol/L(S/N=3). The fabrication method of this sensor, which has highly sensitive, low working potential, and fast amperometric sensing to ascorbic acid, is simple and without using complex equipment. In addition, the sensor has been successfully used to detect ascorbic acid in real sample, thus is promising for the future development of ascorbic acid sensors.


2019 ◽  
Vol 14 (3) ◽  
pp. 171-176 ◽  
Author(s):  
Fanglong Yuan ◽  
Ya-Kun Wang ◽  
Geetu Sharma ◽  
Yitong Dong ◽  
Xiaopeng Zheng ◽  
...  

2013 ◽  
Vol 5 (24) ◽  
pp. 13242-13247 ◽  
Author(s):  
Min Zheng ◽  
Zhigang Xie ◽  
Dan Qu ◽  
Di Li ◽  
Peng Du ◽  
...  

2013 ◽  
Vol 562-565 ◽  
pp. 813-816
Author(s):  
Jia Hong He ◽  
Zhi Qiang Gao ◽  
Zhong Rong Song

A Platinum nanoparticles modified Au electrode has been successfully fabricated by using an in situ growth method. In this method, the Platinum nanoparticles could be grown on the Au electrode surface via the one-step immersion into the mixture of H2PtCl6 (analytical grade, 1g/L), NaBH4 (analytical grade) and polyvinylpyrrolidone K30 (PVP, analytical grade). A certain amount of PVP was added into the reaction system to prevent the coagulation of the Platinum nanoparticles, which obtained by the chemical redox reaction of H2PtCl6 and NaBH4. The structures and morphologies of the Platinum nanoparticles were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) . The direct electrochemical behavior of ascorbic acid in 0.3 mol/L NaCl medium at the Platinum nanoparticles modified electrode has been investigated in detail. Compared to a bare Au electrode, a substantial decrease in the overvoltage of the ascorbic acid was observed at the Platinum nanoparticles modified electrode with oxidation starting at ca. 0.20 V vs. SCE (saturated KCl). At an applied potential of 0.18V, this modified electrode produced high and reproducible sensitivity to ascorbic acid and linear responses were obtained over a concentration range from 0.600 to 3.267 μmol/L with a detection limit of 1.9 nmol/L(S/N=3). The fabrication method of this sensor, which has highly sensitive, low working potential, and fast amperometric sensing to ascorbic acid, is simple and without using complex equipment. In addition, the sensor has been successfully used to detect ascorbic acid in real sample, thus is promising for the future development of ascorbic acid sensors.


The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6609-6616 ◽  
Author(s):  
Yan Huang ◽  
Na He ◽  
Qi Kang ◽  
Dazhong Shen ◽  
Xiaoyan Wang ◽  
...  

The fluorescent nanoprobe CD-DB complexation with iron ions was used to determine the fluctuation of ascorbic acid induced by hypoxia in living cells, zebrafish and mice.


2016 ◽  
Vol 15 (10) ◽  
pp. 1282-1288 ◽  
Author(s):  
Houcem Maaoui ◽  
Pawan Kumar ◽  
Anurag Kumar ◽  
Guo-Hui Pan ◽  
Radouane Chtourou ◽  
...  

Prussian blue/carbon dot (PB/CD) nanocomposite was found to be a visible light active photocatalyst for the oxidative cyanation of tertiary amines using NaCN/AcOH as cyanide source and H2O2 as an oxidant.


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