Efficient strand displacement amplification via stepwise movement of a bipedal DNA walker on an electrode surface for ultrasensitive detection of antibiotics

The Analyst ◽  
2020 ◽  
Vol 145 (8) ◽  
pp. 2975-2981 ◽  
Author(s):  
Rufeng Zhang ◽  
Jie Zhang ◽  
Xiaonan Qu ◽  
Shasha Li ◽  
Yihan Zhao ◽  
...  
Keyword(s):  
Electrode Surface ◽  
Ultrasensitive Detection ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Dna Walker

Efficient strand displacement amplification via stepwise movement of bipedal DNA walker on electrode surface for ultrasensitive detection of antibiotic.

scholarly journals An integrated-molecular-beacon based multiple exponential strand displacement amplification strategy for ultrasensitive detection of DNA methyltransferase activity

2019 ◽  
Vol 10 (8) ◽  
pp. 2290-2297 ◽  
Author(s):  
Yun-Xi Cui ◽  
Xue-Nan Feng ◽  
Ya-Xin Wang ◽  
Hui-Yu Pan ◽  
Hua Pan ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Molecular Beacon ◽  
Ultrasensitive Detection ◽  
Strand Displacement ◽  
Methyltransferase Activity ◽  
Strand Displacement Amplification ◽  
Multiple Signal ◽  
Amplification Strategy

An ultra-sensitive biosensor using only two DNA oligos to initiate multiple signal amplification cycles.

Target–aptamer binding triggered quadratic recycling amplification for highly specific and ultrasensitive detection of antibiotics at the attomole level

2015 ◽  
Vol 51 (39) ◽  
pp. 8377-8380 ◽  
Author(s):  
Hongzhi Wang ◽  
Yu Wang ◽  
Su Liu ◽  
Jinghua Yu ◽  
Wei Xu ◽  
...  
Keyword(s):  
Ultrasensitive Detection ◽  
Electrochemical Aptasensor ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Target Recycling ◽  
Nicking Endonuclease ◽  
Recycling Amplification

A novel electrochemical aptasensor for ultrasensitive detection of antibiotics by combining polymerase-assisted target recycling amplification with strand displacement amplification with the help of polymerase and nicking endonuclease has been reported.

scholarly journals Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 602
Author(s):  
Sandra Leonardo ◽  
Anna Toldrà ◽  
Mònica Campàs
Keyword(s):  
Food Safety ◽  
Environmental Monitoring ◽  
Rolling Circle Amplification ◽  
Dna Amplification ◽  
Isothermal Amplification ◽  
In Situ Monitoring ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Bacterial Detection ◽  
Efficient Detection

The easy and rapid spread of bacterial contamination and the risk it poses to human health makes evident the need for analytical methods alternative to conventional time-consuming laboratory-based techniques for bacterial detection. To tackle this demand, biosensors based on isothermal DNA amplification methods have emerged, which avoid the need for thermal cycling, thus facilitating their integration into small and low-cost devices for in situ monitoring. This review focuses on the breakthroughs made on biosensors based on isothermal amplification methods for the detection of bacteria in the field of food safety and environmental monitoring. Optical and electrochemical biosensors based on loop mediated isothermal amplification (LAMP), rolling circle amplification (RCA), recombinase polymerase amplification (RPA), helicase dependent amplification (HDA), strand displacement amplification (SDA), and isothermal strand displacement polymerisation (ISDPR) are described, and an overview of their current advantages and limitations is provided. Although further efforts are required to harness the potential of these emerging analytical techniques, the coalescence of the different isothermal amplification techniques with the wide variety of biosensing detection strategies provides multiple possibilities for the efficient detection of bacteria far beyond the laboratory bench.

Ultra-sensitive MicroRNA-21 detection based on multiple cascaded strand displacement amplification and CRISPR/Cpf1 (MC-SDA/CRISPR/Cpf1)

2021 ◽  
Author(s):  
Xiaolong Chen ◽  
Yuanyi Deng ◽  
Gaihua Cao ◽  
Yifan Xiong ◽  
Danqun Huo ◽  
...  
Keyword(s):  
Cancer Diagnosis ◽  
Analytical Method ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Diagnosis And Prognosis ◽  
Potential Biomarker ◽  
Cancer Diagnosis And Prognosis

MicroRNA-21 (miR-21) has been considered as a potential biomarker for cancer diagnosis and prognosis due to its highly expressed in tumors. Here, an analytical method which integrates the multiple cascaded...

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