A photoluminescence biosensor for the detection of N-acyl homoserine lactone using cysteamine functionalized ZnO nanoparticles for the early diagnosis of urinary tract infections

2020 ◽  
Vol 8 (19) ◽  
pp. 4228-4236
Author(s):  
Sahana Vasudevan ◽  
Parthasarathy Srinivasan ◽  
John Bosco Balaguru Rayappan ◽  
Adline Princy Solomon
Keyword(s):  
Urinary Tract ◽  
Early Diagnosis ◽  
Urinary Tract Infections ◽  
Zno Nanoparticles ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones ◽  
Tract Infections ◽  
Quorum Sensing Signals

We report a PL biosensor using cysteamine functionalized ZnO nanoparticles for the detection of quorum sensing signals (N-acyl homoserine lactones).

scholarly journals Metabolism of Acyl-Homoserine Lactone Quorum-Sensing Signals by Variovorax paradoxus

2000 ◽  
Vol 182 (24) ◽  
pp. 6921-6926 ◽  
Author(s):  
Jared R. Leadbetter ◽  
E. P. Greenberg
Keyword(s):  
Energy Source ◽  
Nitrogen Sources ◽  
Homoserine Lactone ◽  
Sole Source ◽  
Acyl Homoserine Lactone ◽  
Homoserine Lactones ◽  
Variovorax Paradoxus ◽  
Acyl Homoserine Lactones ◽  
Quorum Sensing Signals ◽  
Sole Energy

ABSTRACT Acyl-homoserine lactones (acyl-HSLs) serve as dedicated cell-to-cell signaling molecules in many species of the classProteobacteria. We have addressed the question of whether these compounds can be degraded biologically. A motile, rod-shaped bacterium was isolated from soil based upon its ability to utilizeN-(3-oxohexanoyl)-l-homoserine lactone as the sole source of energy and nitrogen. The bacterium was classified as a strain of Variovorax paradoxus. TheV. paradoxus isolate was capable of growth on all of the acyl-HSLs tested. The molar growth yields correlated with the length of the acyl group. HSL, a product of acyl-HSL metabolism, was used as a nitrogen source, but not as an energy source. Cleavage and partial mineralization of the HSL ring were demonstrated by using radiolabeled substrate. This study indicates that some strains of V. paradoxus degrade and grow on acyl-HSL signals as the sole energy and nitrogen sources. This study provides clues about the metabolic pathway of acyl-HSL degradation by V. paradoxus.

scholarly journals Evidence for Acyl Homoserine Lactone Signal Production in Bacteria Associated with Marine Sponges

2004 ◽  
Vol 70 (7) ◽  
pp. 4387-4389 ◽  
Author(s):  
Michael W. Taylor ◽  
Peter J. Schupp ◽  
Harriet J. Baillie ◽  
Timothy S. Charlton ◽  
Rocky de Nys ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Bacterial Colonization ◽  
Homoserine Lactone ◽  
Marine Sponges ◽  
Acyl Homoserine Lactone ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones ◽  
Signal Production ◽  
First Time ◽  
Quorum Sensing Signals

ABSTRACT We report for the first time the production of acyl homoserine lactones (AHLs) by bacteria associated with marine sponges. Given the involvement of AHLs in bacterial colonization of many higher organisms, we speculate that such quorum sensing signals could play a part in interactions between sponges and the dense bacterial communities living within them.

MP61-14 EFFECT OF NEWBORN CIRCUMCISION ON URINARY TRACT INFECTIONS IN PATIENTS WITH AN EARLY DIAGNOSIS OF HYDRONEPHROSIS.

2017 ◽  
Vol 197 (4S) ◽  
Author(s):  
Jonathan Ellison ◽  
Geolani Dy ◽  
Ben Fu ◽  
Sarah Holt ◽  
John Gore ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Early Diagnosis ◽  
Urinary Tract Infections ◽  
Tract Infections

scholarly journals Two Dissimilar N-Acyl-Homoserine Lactone Acylases of Pseudomonas syringae Influence Colony and Biofilm Morphology

2008 ◽  
Vol 75 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Ryan W. Shepherd ◽  
Steven E. Lindow
Keyword(s):  
Pseudomonas Syringae ◽  
Biofilm Formation ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Associated Bacteria ◽  
Homoserine Lactones ◽  
Amide Bonds ◽  
Acyl Homoserine Lactones ◽  
Complex Habitat ◽  
Long Chain

ABSTRACT Plant aerial surfaces comprise a complex habitat for microorganisms, and many plant-associated bacteria, such as the pathogen Pseudomonas syringae, exhibit density-dependent survival on leaves by utilizing quorum sensing (QS). QS is often mediated by diffusible signals called N-acyl-homoserine lactones (AHLs), and P. syringae utilizes N-3-oxo-hexanoyl-dl-homoserine lactone (3OC6HSL) to control traits influencing epiphytic fitness and virulence. The P. syringae pathovar syringae B728a genome sequence revealed two putative AHL acylases, termed HacA (Psyr_1971) and HacB (Psyr_4858), which are N-terminal nucleophile hydrolases that inactivate AHLs by cleaving their amide bonds. HacA is a secreted AHL acylase that degrades only long-chain (C ≥ 8) AHLs, while HacB is not secreted and degrades all tested AHLs. Targeted disruptions of hacA, hacB, and hacA and hacB together do not alter endogenous 3OC6HSL levels under the tested conditions. Surprisingly, targeted disruptions of hacA alone and hacA and hacB together confer complementable phenotypes that are very similar to autoaggregative phenotypes seen in other species. While AHL acylases might enable P. syringae B728a to degrade signals of competing species and block expression of their QS-dependent traits, these enzymes also play fundamental roles in biofilm formation.

scholarly journals Long-Chain Acyl-Homoserine Lactone Quorum-Sensing Regulation of Rhodobacter capsulatus Gene Transfer Agent Production

2002 ◽  
Vol 184 (23) ◽  
pp. 6515-6521 ◽  
Author(s):  
Amy L. Schaefer ◽  
Terumi A. Taylor ◽  
J. Thomas Beatty ◽  
E. P. Greenberg
Keyword(s):  
Gene Transfer ◽  
Quorum Sensing ◽  
Rhodobacter Capsulatus ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones ◽  
Chain Acyl ◽  
Gene Transfer Agent ◽  
Long Chain

ABSTRACT Many proteobacteria use acyl-homoserine lactones as quorum-sensing signals. Traditionally, biological detection systems have been used to identify bacteria that produce acyl-homoserine lactones, although the specificities of these detection systems can limit discovery. We used a sensitive approach that did not require a bioassay to detect production of long-acyl-chain homoserine lactone production by Rhodobacter capsulatus and Paracoccus denitrificans. These long-chain acyl-homoserine lactones are not readily detected by standard bioassays. The most abundant acyl-homoserine lactone was N-hexadecanoyl-homoserine lactone. The long-chain acyl-homoserine lactones were concentrated in cells but were also found in the culture fluid. An R. capsulatus gene responsible for long-chain acyl-homoserine lactone synthesis was identified. A mutation in this gene, which we named gtaI, resulted in decreased production of the R. capsulatus gene transfer agent, and gene transfer agent production was restored by exogenous addition of N-hexadecanoyl-homoserine lactone. Thus, long-chain acyl-homoserine lactones serve as quorum-sensing signals to enhance genetic exchange in R. capsulatus.

scholarly journals The GCR1 and GPA1 Participate in Promotion of Arabidopsis Primary Root Elongation Induced by N-Acyl-Homoserine Lactones, the Bacterial Quorum-Sensing Signals

2012 ◽  
Vol 25 (5) ◽  
pp. 677-683 ◽  
Author(s):  
Fang Liu ◽  
Ziriu Bian ◽  
Zhenhua Jia ◽  
Qian Zhao ◽  
Shuishan Song
Keyword(s):  
Quorum Sensing ◽  
Root Elongation ◽  
Primary Root ◽  
Homoserine Lactone ◽  
Collective Behaviors ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Quorum Sensing Signals

Many gram-negative bacteria use N-acyl-homoserine lactones (AHL) as quorum-sensing signals to coordinate their collective behaviors. Accumulating evidence indicates that plants can respond to AHL. However, little is known about the molecular mechanism of plants reacting to these bacterial signals. In this study, we show that the treatment of Arabidopsis roots with N-3-oxo-hexanoyl-homoserine lactone (3OC6-HSL) and N-3-oxo-octanoyl-homoserine lactone (3OC8-HSL) resulted in significant root elongation. The genetic analysis revealed that the T-DNA insertional mutants of gcr1, encoding a G-protein-coupled receptor GCR1, were insensitive to 3OC6-HSL or 3OC8-HSL in assays of root growth. The loss-of-function mutants of the sole canonical Gα subunit GPA1 showed no response to AHL promotion of root elongation whereas Gα gain-of-function plants overexpressing either the wild type or a constitutively active version of Arabidopsis Gα exhibited the exaggerated effect on root elongation caused by AHL. Furthermore, the expression of GCR1 and GPA1 were significantly upregulated after plants were contacted with both AHL. Taken together, our results suggest that GCR1 and GPA1 are involved in AHL-mediated elongation of Arabidopsis roots. This provides insight into the mechanism of plant responses to bacterial quorum-sensing signals.

scholarly journals Biofilm activity, ammonia removal and cell growth of the heterotrophic nitrifier,Acinetobactersp., facilitated by exogenousN-acyl-homoserine lactones

RSC Advances ◽  
2018 ◽  
Vol 8 (54) ◽  
pp. 30783-30793 ◽  
Author(s):  
Xiujie Wang ◽  
Weiqi Wang ◽  
Yun Li ◽  
Jing Zhang ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Cell Growth ◽  
Nitrogen Removal ◽  
Homoserine Lactone ◽  
Ammonia Removal ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Acyl Homoserine Lactone ◽  
Homoserine Lactones ◽  
Removal Process ◽  
Acyl Homoserine Lactones

In the present study, the heterotrophic nitrification–aerobic denitrification strain,Acinetobactersp. JQ1004, was treated with three typicalN-acyl-homoserine lactone (AHL) molecules (C6-HSL, C8-HSL, and 3-oxo-C10-HSL) during the nitrogen removal process.

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