One-step sensitive thrombin detection based on a nanofibrous sensing platform

2019 ◽  
Vol 7 (34) ◽  
pp. 5161-5169 ◽  
Author(s):  
Xiaoling Li ◽  
Yuyang Wu ◽  
Jingjing Niu ◽  
Dagang Jiang ◽  
Dan Xiao ◽  
...  
Keyword(s):  
Nanofibrous Membrane ◽  
Fluorescent Detection ◽  
Sensing Platform ◽  
One Step ◽  
Testing Solution

By integration of a new PiDSD process, CHA amplification and ThT binding, one-step fluorescent detection of thrombin can be achieved by immersing a porous and 3-direction structural B-H2-functionalized nanofibrous membrane in a testing solution.

scholarly journals A selective and sensitive nanosensor for fluorescent detection of specific IgEs to purified allergens in human serum

RSC Advances ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 3547-3555 ◽  
Author(s):  
Jianrong Yun ◽  
Fang Duan ◽  
Liming Liu ◽  
Xiulai Chen ◽  
Jia Liu ◽  
...  
Keyword(s):  
Human Serum ◽  
Fluorescent Detection ◽  
Fluorescence Sensing ◽  
Sensing Platform ◽  
One Step

The effective magnetic fluorescence sensing platform was employed to amplify signal and capture target IgE in one step.

Graphdiyne-Based One-Step DNA Fluorescent Sensing Platform for the Detection of Mycobacterium tuberculosis and Its Drug-Resistant Genes

2019 ◽  
Vol 11 (39) ◽  
pp. 35622-35629 ◽  
Author(s):  
Fan Chang ◽  
Lijun Huang ◽  
Chaozhong Guo ◽  
Guoming Xie ◽  
Jiaqiang Li ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Drug Resistant ◽  
Fluorescent Sensing ◽  
Resistant Genes ◽  
Sensing Platform ◽  
One Step

One-step recovery of noble metal ions from oil/water emulsions by chitin nanofibrous membrane for further recycling utilization

2019 ◽  
Vol 223 ◽  
pp. 115064 ◽  
Author(s):  
Zhiguo Wang ◽  
Peiyuan Li ◽  
Yan Fang ◽  
Liyu Yan ◽  
Weikang Zhou ◽  
...  
Keyword(s):  
Metal Ions ◽  
Noble Metal ◽  
Nanofibrous Membrane ◽  
One Step ◽  
Oil Water

scholarly journals Aptamer Conformation Switching-Induced Two-Stage Amplification for Fluorescent Detection of Proteins

Sensors ◽  
2018 ◽  
Vol 19 (1) ◽  
pp. 77 ◽  
Author(s):  
Qiao Yu ◽  
Fenfen Zhai ◽  
Hong Zhou ◽  
Zonghua Wang
Keyword(s):  
Molecular Beacon ◽  
Limit Of Detection ◽  
Low Cost ◽  
Dna Aptamer ◽  
Fluorescent Detection ◽  
Fluorescence Signal ◽  
Sensing Platform ◽  
Low Concentrations ◽  
Diagnostic Applications ◽  
Recycling Amplification

Basing on the conformation change of aptamer caused by proteins, a simple and sensitive protein fluorescent assay strategy is proposed, which is assisted by the isothermal amplification reaction of polymerase and nicking endonuclease. In the presence of platelet-derived growth factor (PDGF-BB), the natural conformation of a DNA aptamer would change into a Y-shaped complex, which could hybridize with a molecular beacon (MB) and form a DNA duplex, leading to the open state of the MB and generating a fluorescence signal. Subsequently, with further assistance of isothermal recycling amplification strategies, the designed aptamer sensing platform showed an increment of fluorescence. As a benefit of this amplified strategy, the limit of detection (LOD) was lowered to 0.74 ng/mL, which is much lower than previous reports. This strategy not only offers a new simple, specific, and efficient platform to quantify the target protein in low concentrations, but also shows a powerful approach without multiple washing steps, as well as a precious implementation that has the potential to be integrated into portable, low-cost, and simplified devices for diagnostic applications.

scholarly journals Hydration-Enhanced Lubricating Electrospun Nanofibrous Membranes Prevent Tissue Adhesion

Research ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-12 ◽  
Author(s):  
Liang Cheng ◽  
Yi Wang ◽  
Guoming Sun ◽  
Shizhu Wen ◽  
Lianfu Deng ◽  
...  
Keyword(s):  
Comfort Level ◽  
Nanofibrous Membrane ◽  
Tissue Adhesion ◽  
Modification Method ◽  
The Coefficient Of Friction ◽  
One Step ◽  
Biomedical Field ◽  
Resistance Performance

Lubrication is the key to efficient function of human tissues and has significant impact on the comfort level. However, the construction of a lubricating nanofibrous membrane has not been reported as yet, especially using a one-step surface modification method. Here, bioinspired by the superlubrication mechanism of articular cartilage, we successfully construct hydration-enhanced lubricating nanofibers via one-step in situ grafting of a copolymer synthesized by dopamine methacrylamide (DMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC) onto electrospun polycaprolactone (PCL) nanofibers. The zwitterionic MPC structure provides the nanofiber surface with hydration lubrication behavior. The coefficient of friction (COF) of the lubricating nanofibrous membrane decreases significantly and is approximately 65% less than that of pure PCL nanofibers, which are easily worn out under friction regardless of hydration. The lubricating nanofibers, however, show favorable wear-resistance performance. Besides, they possess a strong antiadhesion ability of fibroblasts compared with pure PCL nanofibers. The cell density decreases approximately 9-fold, and the cell area decreases approximately 12 times on day 7. Furthermore, the in vivo antitendon adhesion data reveals that the lubricating nanofiber group has a significantly lower adhesion score and a better antitissue adhesion. Altogether, our developed hydration-enhanced lubricating nanofibers show promising applications in the biomedical field such as antiadhesive membranes.

Facile preparation of N,S-graphene oxide nanosheets as a fluorescence “off–on” sensing platform for sensitive detection of biothiols

2019 ◽  
Vol 43 (6) ◽  
pp. 2790-2796 ◽  
Author(s):  
Xiuxiu Zhao ◽  
Xin Lin ◽  
Jianhua Wang ◽  
Xuwei Chen
Keyword(s):  
Graphene Oxide ◽  
Solvent Free ◽  
Sensitive Detection ◽  
Graphene Oxide Nanosheets ◽  
Sensing Platform ◽  
One Step ◽  
Facile Preparation

Fluorescent N,S-graphene oxide was prepared via a one-step, solvent-free approach, and used as a probe for sensitive detection of biothiols.

One-step nanotopography construction by polyaniline polymerization for a superhydrophobic nanofibrous membrane towards direct contact membrane distillation

2019 ◽  
Vol 6 (8) ◽  
pp. 2553-2564 ◽  
Author(s):  
Lingling Zhong ◽  
Zhigao Zhu ◽  
Yu Han ◽  
Qiao Wang ◽  
Dongmei Liu ◽  
...  
Keyword(s):  
Direct Contact ◽  
Water Flux ◽  
High Water ◽  
Membrane Distillation ◽  
Nanofibrous Membrane ◽  
Direct Contact Membrane Distillation ◽  
One Step ◽  
Nanofibrous Membranes

In situ polymerization of aniline is used to prepare superhydrophobic PVDF nanofibrous membranes for direct contact membrane distillation with high water flux and long wetting times.

Green one-step synthesis of carbon quantum dots from orange peel for fluorescent detection of Escherichia coli in milk

2021 ◽  
Vol 339 ◽  
pp. 127775
Author(s):  
Xuetao Hu ◽  
Yanxiao Li ◽  
Yiwei Xu ◽  
Ziyu Gan ◽  
Xiaobo Zou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quantum Dots ◽  
Orange Peel ◽  
Carbon Quantum Dots ◽  
Fluorescent Detection ◽  
One Step
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