Liquid biopsy in combination with solid-state electrochemical sensors and nucleic acid amplification

2019 ◽  
Vol 7 (43) ◽  
pp. 6655-6669 ◽  
Author(s):  
Miyuki Tabata ◽  
Yuji Miyahara
Keyword(s):  
Solid State ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Liquid Biopsy ◽  
Electrochemical Sensors ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification

Solid-state electrochemical sensors are developing as a new platform for liquid biopsy, combining detection and analysis of nucleic acids with isothermal nucleic acid amplification reactions.

A novel exonuclease-assisted isothermal nucleic acid amplification with ultrahigh specificity mediated by full-length Bst DNA polymerase

2018 ◽  
Vol 54 (75) ◽  
pp. 10562-10565 ◽  
Author(s):  
Xin Ye ◽  
Yang Li ◽  
Lijuan Wang ◽  
Xueen Fang ◽  
Jilie Kong
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Dna Polymerase ◽  
Isothermal Amplification ◽  
Full Length ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification

A novel exonuclease-assisted isothermal amplification to amplify and determine nucleic acids very sensitively and with ultrahigh specificity.

Flap endonuclease-initiated enzymatic repairing amplification for ultrasensitive detection of target nucleic acids

Nanoscale ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 3633-3638 ◽  
Author(s):  
Hyowon Jang ◽  
Chang Yeol Lee ◽  
Seoyoung Lee ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Amplification ◽  
Ultrasensitive Detection ◽  
Isothermal Nucleic Acid Amplification ◽  
Amplification Method

A new isothermal nucleic acid amplification method termed FERA (Flap endonuclease-initiated Enzymatic Repairing Amplification) is developed for the ultrasensitive detection of target nucleic acids.

Electricity-free chemical heater for isothermal nucleic acid amplification with applications in COVID-19 home testing

The Analyst ◽  
2021 ◽  
Author(s):  
Rui Jie Li ◽  
Michael G. Mauk ◽  
Youngung Seok ◽  
Haim H. Bau
Keyword(s):  
Nucleic Acid ◽  
Phase Change ◽  
Phase Change Material ◽  
Heat Generation ◽  
Temperature Regulation ◽  
Nucleic Acid Amplification ◽  
Enzymatic Amplification ◽  
Isothermal Nucleic Acid Amplification ◽  
Home Testing ◽  
Change Material

Electricty-free incubation of isothermal enzymatic amplification with a composite comprised of exothermic reactants for heat generation and phase change material for temperature regulation.

scholarly journals Rapid electrostatic DNA enrichment for sensitive detection of Trichomonas vaginalis in clinical urinary samples

2020 ◽  
Vol 12 (8) ◽  
pp. 1085-1093
Author(s):  
Justin M. Rosenbohm ◽  
James M. Robson ◽  
Rishabh Singh ◽  
Rose Lee ◽  
Jane Y. Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Trichomonas Vaginalis ◽  
Nucleic Acid Amplification ◽  
Urine Samples ◽  
Sensitive Detection ◽  
Isothermal Nucleic Acid Amplification

Rapid electrostatic enrichment of DNA from urine samples for improved isothermal nucleic acid amplification-based detection of Trichomonas vaginalis.

scholarly journals Universal amplification-free molecular diagnostics by billion-fold hierarchical nanofluidic concentration

2019 ◽  
Vol 116 (33) ◽  
pp. 16240-16249 ◽  
Author(s):  
Wei Ouyang ◽  
Jongyoon Han
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Molecular Diagnostics ◽  
Troponin I ◽  
Direct Detection ◽  
Signal To Noise Ratio ◽  
Protein Detection ◽  
Clinical Diagnostics ◽  
Nucleic Acid Amplification ◽  
Enzyme Linked Immunosorbent Assay

Rapid and reliable detection of ultralow-abundance nucleic acids and proteins in complex biological media may greatly advance clinical diagnostics and biotechnology development. Currently, nucleic acid tests rely on enzymatic processes for target amplification (e.g., PCR), which have many inherent issues restricting their implementation in diagnostics. On the other hand, there exist no protein amplification techniques, greatly limiting the development of protein-based diagnosis. We report a universal biomolecule enrichment technique termed hierarchical nanofluidic molecular enrichment system (HOLMES) for amplification-free molecular diagnostics using massively paralleled and hierarchically cascaded nanofluidic concentrators. HOLMES achieves billion-fold enrichment of both nucleic acids and proteins within 30 min, which not only overcomes many inherent issues of nucleic acid amplification but also provides unprecedented enrichment performance for protein analysis. HOLMES features the ability to selectively enrich target biomolecules and simultaneously deplete nontargets directly in complex crude samples, thereby enormously enhancing the signal-to-noise ratio of detection. We demonstrate the direct detection of attomolar nucleic acids in urine and serum within 35 min and HIV p24 protein in serum within 60 min. The performance of HOLMES is comparable to that of nucleic acid amplification tests and near million-fold improvement over standard enzyme-linked immunosorbent assay (ELISA) for protein detection, being much simpler and faster in both applications. We additionally measured human cardiac troponin I protein in 9 human plasma samples, and showed excellent agreement with ELISA and detection below the limit of ELISA. HOLMES is in an unparalleled position to unleash the potential of protein-based diagnosis.

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