Diffusion of nanoparticles within a semidilute polyelectrolyte solution

Soft Matter ◽  
2019 ◽  
Vol 15 (38) ◽  
pp. 7616-7622 ◽  
Author(s):  
Kavindya K. Senanayake ◽  
Namita Shokeen ◽  
Ehsan Akbari Fakhrabadi ◽  
Matthew W. Liberatore ◽  
Ashis Mukhopadhyay
Keyword(s):  
Molecular Weight ◽  
Gold Nanoparticles ◽  
High Molecular Weight ◽  
Polyacrylic Acid ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Polyelectrolyte Solution ◽  
Fluorescence Correlation ◽  
Semidilute Solution

We studied the diffusion of charged gold nanoparticles within a semidilute solution of weakly charged polyelectrolyte, polyacrylic acid (PAA) of high molecular weight (Mw = 106 g mol−1) by using fluorescence correlation spectroscopy (FCS).

Coumarin 6, Hypericin, Resorufins, and Flavins: Suitable Chromophores for Fluorescence Correlation Spectroscopy of Biological Molecules

2001 ◽  
Vol 66 (6) ◽  
pp. 855-869 ◽  
Author(s):  
Martin Beneš ◽  
Jiří Hudeček ◽  
Pavel Anzenbacher ◽  
Martin Hof
Keyword(s):  
Molecular Weight ◽  
Cytochrome P450 ◽  
Fluorescence Correlation Spectroscopy ◽  
Laser Power ◽  
Slow Component ◽  
Correlation Spectroscopy ◽  
Flavin Mononucleotide ◽  
Cytochrome P450 3A4 ◽  
Fluorescence Correlation ◽  
Coumarin 6

In this work we show that the dyes coumarin 6, hypericin, 7-O-ethylresorufin and resorufin are suitable for fluorescence correlation spectroscopy (FCS) and demonstrate the use of these dyes in physiologically relevant protein studies. Since coumarins are metabolised by cytochromes P450, the binding of coumarin 6 to cytochrome P450 3A4 was investigated by FCS. Coumarin 6 appears to be a very bright non-covalent cytochrome P450 label. When titrating cytochrome P450 3A4 with coumarin 6, the diffusion time of the coumarin 6/ cytochrome P450 3A4 complex increases roughly two-fold at protein concentrations higher than 1 μmol l-1, indicating the formation of cytochrome aggregates. FCS of the flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) shows that both endogenous dyes undergo photobleaching. Moreover, FAD appears to be present to great extent, as a non-fluorescent intramolecular complex. Analysis of the FCS data of the flavoprotein NADPH-cytochrome P450 oxidoreductase (molecular weight 76 500) yielded two components. While the slow component corresponds to a globular protein with the molecular weight about 75 000, the fast component appears to be due to free diffusing FMN and FAD molecules. The amount of free FMN and FAD increases with increasing laser power. At high laser power a complete photodissociation of FMN and FAD occurs.

Fluorescence Correlation Spectroscopy of Gold Nanoparticles

2012 ◽  
Vol 45 (1) ◽  
pp. 22-28 ◽  
Author(s):  
P. Sri Balaji ◽  
A. V. R. Murthy ◽  
Neha Tiwari ◽  
Sulabha Kulkarni
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

A sensitive assay of mercury using fluorescence correlation spectroscopy of gold nanoparticles

Luminescence ◽  
2014 ◽  
Vol 30 (5) ◽  
pp. 605-610 ◽  
Author(s):  
Zhancheng Xu ◽  
Tao Lan ◽  
Xiangyi Huang ◽  
Chaoqing Dong ◽  
Jicun Ren
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Sensitive Assay ◽  
Fluorescence Correlation

A Quantitative Study of the Intracellular Dynamics of Fluorescently Labelled Glyco-Gold Nanoparticles via Fluorescence Correlation Spectroscopy

Small ◽  
2014 ◽  
Vol 10 (13) ◽  
pp. 2602-2610 ◽  
Author(s):  
Richard A. Murray ◽  
Yuan Qiu ◽  
Fabrizio Chiodo ◽  
Marco Marradi ◽  
Soledad Penadés ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Quantitative Study ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

scholarly journals Influence of surface coating on the intracellular behaviour of gold nanoparticles: a fluorescence correlation spectroscopy study

Nanoscale ◽  
2017 ◽  
Vol 9 (38) ◽  
pp. 14730-14739 ◽  
Author(s):  
A. Silvestri ◽  
D. Di Silvio ◽  
I. Llarena ◽  
R. A. Murray ◽  
M. Marelli ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Correlation Spectroscopy ◽  
Surface Coating ◽  
Correlation Spectroscopy ◽  
Spectroscopy Study ◽  
Fluorescence Correlation ◽  
Antifouling Coatings ◽  
Gold Nps ◽  
Intracellular Fate

Fluorescence correlation spectroscopy allows the correlation of the state of aggregation in vitro with the intracellular fate of gold NPs for different antifouling coatings.

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