scholarly journals Influence of surface coating on the intracellular behaviour of gold nanoparticles: a fluorescence correlation spectroscopy study

Nanoscale ◽  
2017 ◽  
Vol 9 (38) ◽  
pp. 14730-14739 ◽  
Author(s):  
A. Silvestri ◽  
D. Di Silvio ◽  
I. Llarena ◽  
R. A. Murray ◽  
M. Marelli ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Correlation Spectroscopy ◽  
Surface Coating ◽  
Correlation Spectroscopy ◽  
Spectroscopy Study ◽  
Fluorescence Correlation ◽  
Antifouling Coatings ◽  
Gold Nps ◽  
Intracellular Fate

Fluorescence correlation spectroscopy allows the correlation of the state of aggregation in vitro with the intracellular fate of gold NPs for different antifouling coatings.

scholarly journals A Comprehensive Review of Fluorescence Correlation Spectroscopy

2021 ◽  
Vol 9 ◽  
Author(s):  
Lan Yu ◽  
Yunze Lei ◽  
Ying Ma ◽  
Min Liu ◽  
Juanjuan Zheng ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Fluorescence Correlation Spectroscopy ◽  
Pair Correlation ◽  
Time Correlation ◽  
Correlation Spectroscopy ◽  
Local Concentration ◽  
Fluorescence Correlation ◽  
Pros And Cons

Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of molecular dynamics, and it has been widely applied in diverse fields, e.g., biomedicine, biophysics, and chemistry. By time-correlation of the fluorescence fluctuations induced by molecules diffusing through a focused light, FCS can quantitatively evaluate the concentration, diffusion coefficient, and interaction of the molecules in vitro or in vivo. In this review, the basic principle and implementation of FCS are introduced. Then, the advances of FCS variants are reviewed, covering dual-color FCCS, multi-focus FCS, pair correlation function (pCF), scanning FCS, focus-reduced FCS, SPIM-FCS, and inverse-FCS. Besides, the applications of FCS are demonstrated with the measurement of local concentration, hydrodynamic radius, diffusion coefficient, and the interaction of different molecules. Lastly, a discussion is given by summarizing the pros and cons of different FCS techniques, as well as the outlooks and perspectives of FCS.

Fluorescence Correlation Spectroscopy of Gold Nanoparticles

2012 ◽  
Vol 45 (1) ◽  
pp. 22-28 ◽  
Author(s):  
P. Sri Balaji ◽  
A. V. R. Murthy ◽  
Neha Tiwari ◽  
Sulabha Kulkarni
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

scholarly journals The oligomeric state and stability of the mannitol transporter, EnzymeIImtl, fromEscherichia coli: A fluorescence correlation spectroscopy study

2006 ◽  
Vol 15 (8) ◽  
pp. 1977-1986 ◽  
Author(s):  
Gertjan Veldhuis ◽  
Mark Hink ◽  
Victor Krasnikov ◽  
Geert van den Bogaart ◽  
Jeroen Hoeboer ◽  
...  
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Spectroscopy Study ◽  
Oligomeric State ◽  
Fluorescence Correlation ◽  
Fromescherichia Coli

scholarly journals Antenna Protein Clustering In Vitro Unveiled by Fluorescence Correlation Spectroscopy

2021 ◽  
Vol 22 (6) ◽  
pp. 2969
Author(s):  
Aurélie Crepin ◽  
Edel Cunill-Semanat ◽  
Eliška Kuthanová Trsková ◽  
Erica Belgio ◽  
Radek Kaňa
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Higher Plants ◽  
Fluorescence Emission ◽  
Correlation Spectroscopy ◽  
Photochemical Quenching ◽  
High Ph ◽  
Fluorescence Correlation ◽  
Non Photochemical Quenching

Antenna protein aggregation is one of the principal mechanisms considered effective in protecting phototrophs against high light damage. Commonly, it is induced, in vitro, by decreasing detergent concentration and pH of a solution of purified antennas; the resulting reduction in fluorescence emission is considered to be representative of non-photochemical quenching in vivo. However, little is known about the actual size and organization of antenna particles formed by this means, and hence the physiological relevance of this experimental approach is questionable. Here, a quasi-single molecule method, fluorescence correlation spectroscopy (FCS), was applied during in vitro quenching of LHCII trimers from higher plants for a parallel estimation of particle size, fluorescence, and antenna cluster homogeneity in a single measurement. FCS revealed that, below detergent critical micelle concentration, low pH promoted the formation of large protein oligomers of sizes up to micrometers, and therefore is apparently incompatible with thylakoid membranes. In contrast, LHCII clusters formed at high pH were smaller and homogenous, and yet still capable of efficient quenching. The results altogether set the physiological validity limits of in vitro quenching experiments. Our data also support the idea that the small, moderately quenching LHCII oligomers found at high pH could be relevant with respect to non-photochemical quenching in vivo.

Diffusion of nanoparticles within a semidilute polyelectrolyte solution

Soft Matter ◽  
2019 ◽  
Vol 15 (38) ◽  
pp. 7616-7622 ◽  
Author(s):  
Kavindya K. Senanayake ◽  
Namita Shokeen ◽  
Ehsan Akbari Fakhrabadi ◽  
Matthew W. Liberatore ◽  
Ashis Mukhopadhyay
Keyword(s):  
Molecular Weight ◽  
Gold Nanoparticles ◽  
High Molecular Weight ◽  
Polyacrylic Acid ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Polyelectrolyte Solution ◽  
Fluorescence Correlation ◽  
Semidilute Solution

We studied the diffusion of charged gold nanoparticles within a semidilute solution of weakly charged polyelectrolyte, polyacrylic acid (PAA) of high molecular weight (Mw = 106 g mol−1) by using fluorescence correlation spectroscopy (FCS).

Sign in / Sign up

Export Citation Format

Share Document