scholarly journals Design, synthesis and evaluation of novel, branched trident small interfering RNA nanostructures for sequence-specific RNAi activity

RSC Advances ◽  
2019 ◽  
Vol 9 (59) ◽  
pp. 34166-34171
Author(s):  
Akash Chandela ◽  
Yoshihito Ueno
Keyword(s):  
Gene Silencing ◽  
Small Interfering Rna ◽  
Target Gene ◽  
Physical Characterization ◽  
Small Interfering Rnas ◽  
Systemic Delivery ◽  
Design Synthesis ◽  
Serum Stability ◽  
Interfering Rna ◽  
Rnai Activity

Branched small interfering RNAs (siRNAs) are potential candidates for on-target gene silencing with enhanced serum stability. Their physical characterization also presents them as a prospective drug for systemic delivery.

Optimization of a synthetic siRNA delivery for the treatment of rhabdomyosarcoma

2008 ◽  
Vol 3 (4) ◽  
pp. 371-379 ◽  
Author(s):  
Ewa Lesko ◽  
Katarzyna Miekus ◽  
Agnieszka Grabowska ◽  
Aleksandra Gładys ◽  
Marcin Majka
Keyword(s):  
Gene Silencing ◽  
Small Interfering Rna ◽  
Target Gene ◽  
Target Genes ◽  
Sirna Delivery ◽  
Basic Research ◽  
Delivery Strategy ◽  
Therapeutic Benefits ◽  
Interfering Rna

AbstractSilencing of a target-genes by small interfering RNA (siRNA) has emerged as a powerful new tool not only for basic research but also with potential therapeutic benefits. This paper demonstrates that optimal delivery strategy is crucial for effective target-gene silencing. Using lipofection, under defined conditions, we were able to markedly down-regulate expression of the selected genes involved in rhabdomyosarcoma metastasis: MET, CXCR4, LIFR and PAX3-FKHR.

Targeted Gene Silencing by Small Interfering RNA-Based Knock-Down Technology

2004 ◽  
Vol 5 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Bentham Science Publisher Zhang J. ◽  
Bentham Science Publisher Hua Z.C.
Keyword(s):  
Gene Silencing ◽  
Small Interfering Rna ◽  
Knock Down ◽  
Interfering Rna

Pre-Injection of Small Interfering RNA (siRNA) Promotes c-Jun Gene Silencing and Decreases the Survival Rate of Axotomy-Injured Spinal Motoneurons in Adult Mice

2018 ◽  
Vol 65 (3) ◽  
pp. 400-410 ◽  
Author(s):  
Ying-qin Li ◽  
Fa-huan Song ◽  
Ke Zhong ◽  
Guang-yin Yu ◽  
Prince Last Mudenda Zilundu ◽  
...  
Keyword(s):  
Survival Rate ◽  
Gene Silencing ◽  
Small Interfering Rna ◽  
Spinal Motoneurons ◽  
Adult Mice ◽  
Interfering Rna

scholarly journals Carbon Dots for Efficient Small Interfering RNA Delivery and Gene Silencing in Plants

2020 ◽  
Vol 184 (2) ◽  
pp. 647-657 ◽  
Author(s):  
Steven H. Schwartz ◽  
Bill Hendrix ◽  
Paul Hoffer ◽  
Rick A. Sanders ◽  
Wei Zheng
Keyword(s):  
Gene Silencing ◽  
Carbon Dots ◽  
Small Interfering Rna ◽  
Interfering Rna

scholarly journals Switching the intracellular pathway and enhancing the therapeutic efficacy of small interfering RNA by auroliposome

2020 ◽  
Vol 6 (30) ◽  
pp. eaba5379 ◽  
Author(s):  
Md. Nazir Hossen ◽  
Lin Wang ◽  
Harisha R. Chinthalapally ◽  
Joe D. Robertson ◽  
Kar-Ming Fung ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gold Nanoparticles ◽  
Gene Silencing ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Ovarian Cancer Cells ◽  
Interfering Rna

Gene silencing using small-interfering RNA (siRNA) is a viable therapeutic approach; however, the lack of effective delivery systems limits its clinical translation. Herein, we doped conventional siRNA-liposomal formulations with gold nanoparticles to create “auroliposomes,” which significantly enhanced gene silencing. We targeted MICU1, a novel glycolytic switch in ovarian cancer, and delivered MICU1-siRNA using three delivery systems—commercial transfection agents, conventional liposomes, and auroliposomes. Low-dose siRNA via transfection or conventional liposomes was ineffective for MICU1 silencing; however, in auroliposomes, the same dose gave >85% gene silencing. Efficacy was evident from both in vitro growth assays of ovarian cancer cells and in vivo tumor growth in human ovarian cell line—and patient-derived xenograft models. Incorporation of gold nanoparticles shifted intracellular uptake pathways such that liposomes avoided degradation within lysosomes. Auroliposomes were nontoxic to vital organs. Therefore, auroliposomes represent a novel siRNA delivery system with superior efficacy for multiple therapeutic applications.

Small interfering RNA delivery and gene silencing using polymeric nanocarrier systems

2005 ◽  
Vol 1 (3) ◽  
pp. 299-299
Author(s):  
K. A. Howard ◽  
X. Liu ◽  
D. Oupicky ◽  
S. Zoffmann ◽  
F. Besenbacher ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Small Interfering Rna ◽  
Interfering Rna ◽  
Polymeric Nanocarrier

scholarly journals Intravaginal gene silencing using biodegradable polymer nanoparticles densely loaded with small-interfering RNA

2009 ◽  
Vol 8 (6) ◽  
pp. 526-533 ◽  
Author(s):  
Kim A. Woodrow ◽  
Yen Cu ◽  
Carmen J. Booth ◽  
Jennifer K. Saucier-Sawyer ◽  
Monica J. Wood ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Biodegradable Polymer ◽  
Small Interfering Rna ◽  
Polymer Nanoparticles ◽  
Interfering Rna
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