scholarly journals Preparative separation of seven phenolic acids from Xanthii Fructus using pH-zone-refining counter-current chromatography combined with semi-preparative high performance liquid chromatography

RSC Advances ◽  
2019 ◽  
Vol 9 (63) ◽  
pp. 36524-36529
Author(s):  
Tianyu Ma ◽  
Lihua Xu ◽  
Xinming Wang ◽  
Jia Li ◽  
Lanping Guo ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
High Performance ◽  
Preparative Hplc ◽  
Zone Refining ◽  
Preparative Separation ◽  
Caffeoylquinic Acid ◽  
Counter Current

Segmentation and purification strategy using pH-zone-refining counter-current chromatography combined with semi-preparative HPLC for separation of six caffeoylquinic acid isomers and a caffeic acid from Xanthii Fructus.

scholarly journals Preparative Separation of Monoterpenoid Indole Alkaloid Epimers from Ervatamia yunnanensis Tsiang by pH-Zone-Refining Counter-Current Chromatography Combined with Preparative High-Performance Liquid Chromatography

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1316 ◽  
Author(s):  
Jie Zhou ◽  
Si-Yu Du ◽  
Hong-Jing Dong ◽  
Lei Fang ◽  
Jin-Hong Feng
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Indole Alkaloid ◽  
2D Nmr ◽  
Zone Refining ◽  
Preparative Separation ◽  
Separation And Purification ◽  
Monoterpenoid Indole Alkaloid ◽  
Counter Current

An effective method was developed for the preparative separation and purification of monoterpenoid indole alkaloid epimers from Ervatamia yunnanensis Tsiang using a combination of pH-zone-refining counter-current chromatography and preparative high-performance liquid chromatography. With this method, two pairs of MIA epimers including ervatamine (72 mg, 1), 20-epi-ervatamine (27 mg, 4), dregamine (95 mg, 2), tabernaemontanine (129 mg, 3), along with two MIAs, apparicine (112 mg, 5) and isovoacangine (15 mg, 6), were successfully purified from 2.1 g crude extract of E. yunnanensis, each with a purity of over 95% as determined by HPLC. The structures of the MIAs were identified by ESI-MS, 1D, and 2D NMR.

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