scholarly journals Exposure to microwave irradiation at constant culture temperature slows the growth ofEscherichia coliDE3 cells, leading to modified proteomic profiles

RSC Advances ◽  
2019 ◽  
Vol 9 (21) ◽  
pp. 11810-11817 ◽  
Author(s):  
Sina Atrin Mazinani ◽  
Nour Noaman ◽  
Melissa R. Pergande ◽  
Stephanie M. Cologna ◽  
Jens Coorssen ◽  
...  
Keyword(s):  
Microwave Irradiation ◽  
Culture Temperature ◽  
E Coli

E. coligrowth is slowed by exposure to non-lethal microwave irradiation, accompanied by changes in proteomic profiles.

Microwave Irradiation of PC3 Cells at Constant Culture Temperature Alters the Incorporation of BODIPY into Cells and Reduction of MTT

2017 ◽  
Vol 2 (26) ◽  
pp. 7983-7986 ◽  
Author(s):  
Sina Atrin Mazinani ◽  
Fereshteh Moradi ◽  
Jeffrey A. Stuart ◽  
Hongbin Yan
Keyword(s):  
Microwave Irradiation ◽  
Culture Temperature ◽  
Pc3 Cells

Microwave Synthesis of Nanosilver Colloidal Suspension for Anti-Bacterial Coating

2011 ◽  
Vol 356-360 ◽  
pp. 277-282 ◽  
Author(s):  
Hsi Chi Yang ◽  
Jung Pin Wang ◽  
Chien Te Hsieh
Keyword(s):  
Microwave Irradiation ◽  
Colloidal Suspension ◽  
Bacterial Inactivation ◽  
Narrow Size Distribution ◽  
E Coli ◽  
Microwave Assisted ◽  
Reaction Solution ◽  
Escherchia Coli ◽  
Ag Crystallites ◽  
Heating Up

This article reports a microwave-assisted route to synthesize nanosilver colloidal suspension and to deposit silver nanoparticles onto activated carbon fabrics (ACFs). The properties of the nanosilver suspension are characterized in terms of bacterial inactivation and growth inhibition. The metallic Ag nanocrystals with narrow size distribution are uniformly dispersed onto ACFs under the microwave irradiation of 1 min. Microwave irradiation is capable of heating up the reaction solution homogeneously, inducing uniform nucleation and rapid crystal growth to form the Ag crystallites. This work aims to elucidate how as-grown Ag nanoparticles affect the inactivation of Escherchia coli (E. coli) and how Ag-ACF surface inhibits the bacterial growth. The Ag colloidal suspension offers superior anti-bacterial ability against E. coli cells at a low concentration of 20 mg/L. Thus, the study has established a simple, efficient and effective process in the synthesis of both Ag colloidal suspension and Ag-ACF composite.

Expression of Psychrophilic Genes in Mesophilic Hosts: Assessment of the Folding State of a Recombinant α-Amylase

1998 ◽  
Vol 64 (3) ◽  
pp. 1163-1165 ◽  
Author(s):  
Georges Feller ◽  
Olivier Le Bussy ◽  
Charles Gerday
Keyword(s):  
Escherichia Coli ◽  
Enzyme Stability ◽  
Expression System ◽  
Wild Strain ◽  
Culture Temperature ◽  
E Coli ◽  
System A ◽  
Irreversible Denaturation ◽  
The Antarctic ◽  
Folding State

ABSTRACT α-Amylase from the antarctic psychrophile Alteromonas haloplanktis is synthesized at 0 ± 2°C by the wild strain. This heat-labile α-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the described expression system, a compromise between enzyme stability and E. coli growth rate is reached at 18°C.

scholarly journals The antioxidant and antimicrobial activity for some new synthesized Schiff bases derived from Ascorbic acid

2019 ◽  
Vol 10 (2) ◽  
pp. 1510-1515
Author(s):  
Taiseer Abdul-Kader Saleh ◽  
Rafah Razooq Hameed Al-Samarrai ◽  
Noor Essam Abdul-Razzaq
Keyword(s):  
Ascorbic Acid ◽  
Biological Activity ◽  
Microwave Irradiation ◽  
Schiff Bases ◽  
Traditional Method ◽  
Antioxidant Properties ◽  
Microwave Irradiation Method ◽  
Keto Form ◽  
E Coli ◽  
Antioxidant And Antimicrobial Activity

This study was carried out to synthesise some new Schiff bases compounds through condensation of ascorbic acid (keto form) with some compounds which have amine group in their structure also these compounds synthesized by using of microwave irradiation and traditional method and compare the results obtained. Microwave irradiation of organic reactions has quickly acquired popularity as it increases the speed of the reaction towards multiple kinds of synthetic transformations, solventless procedures without the use of supporting reagents and therefore eco-friendly, the antioxidant properties were studied for all compounds included in this study, the results indicate that IC50 for the compounds synthesized by using of microwave irradiation method were higher than the traditional method, and also the biological activity of the prepared compounds were also studied to estimate capability of suppressing Enterococcus, Staphyllo coccus aureus and E. Coli than the starting materials that have biological activity on these bacteria. This study shows that the diameters of inhibitions in Petri dishes have higher and wider at a concentration (10 mg / ml) for used bacteria.

scholarly journals Unique Plasmids Generated via pUC Replicon Mutagenesis in an Error-Prone Thermophile Derived from Geobacillus kaustophilus HTA426

2015 ◽  
Vol 81 (21) ◽  
pp. 7625-7632 ◽  
Author(s):  
Jyumpei Kobayashi ◽  
Misaki Tanabiki ◽  
Shohei Doi ◽  
Akihiko Kondo ◽  
Takashi Ohshiro ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Plasmid Stability ◽  
Amino Acid Replacement ◽  
Mutant Plasmid ◽  
Culture Temperature ◽  
Gene Encoding ◽  
Chloramphenicol Resistance ◽  
Content Type ◽  
E Coli ◽  
Geobacillus Kaustophilus

ABSTRACTThe plasmid pGKE75-catA138T, which comprises pUC18 and thecatA138Tgene encoding thermostable chloramphenicol acetyltransferase with an A138T amino acid replacement (CATA138T), serves as anEscherichia coli-Geobacillus kaustophilusshuttle plasmid that confers moderate chloramphenicol resistance onG. kaustophilusHTA426. The present study examined the thermoadaptation-directed mutagenesis of pGKE75-catA138Tin an error-prone thermophile, generating the mutant plasmid pGKE75αβ-catA138Tresponsible for substantial chloramphenicol resistance at 65°C. pGKE75αβ-catA138Tcontained no mutation in thecatA138Tgene but had two mutations in the pUC replicon, even though the replicon has no apparent role inG. kaustophilus. Biochemical characterization suggested that the efficient chloramphenicol resistance conferred by pGKE75αβ-catA138Tis attributable to increases in intracellular CATA138Tand acetyl-coenzyme A following a decrease in incomplete forms of pGKE75αβ-catA138T. The decrease in incomplete plasmids may be due to optimization of plasmid replication by RNA species transcribed from the mutant pUC replicon, which were actually produced inG. kaustophilus. It is noteworthy thatG. kaustophiluswas transformed with pGKE75αβ-catA138Tusing chloramphenicol selection at 60°C. In addition, a pUC18 derivative with the two mutations propagated inE. coliat a high copy number independently of the culture temperature and high plasmid stability. Since these properties have not been observed in known plasmids, the outcomes extend the genetic toolboxes forG. kaustophilusandE. coli.

Antibacterial properties of Ag and Ag/AgCl nanoparticles from radish and tea extracts for water treatment applications

2015 ◽  
Vol 16 (1) ◽  
pp. 171-179
Author(s):  
Yuphada Boonto ◽  
Jirapat Ananpattarachai ◽  
Puangrat Kajitvichyanukul
Keyword(s):  
Antibacterial Activity ◽  
Water Treatment ◽  
Microwave Irradiation ◽  
Green Synthesis ◽  
Plant Extracts ◽  
Antibacterial Properties ◽  
Water Disinfection ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Complete Inactivation

Silver nanoparticles (AgNPs) have antibacterial properties and are widely used for water disinfection. This technology is commercially applied in point-of-use water treatment as a post-treatment for filtrate water. However, the current process of synthesizing AgNPs has several disadvantages including the use of hazardous chemicals, consumption of a large amount of energy and the formation of hazardous byproducts. Here, we report an alternative and green synthesis using plant extracts. In this work, the plant extracts came from radish (R) and tea (T), and the AgNPs were derived from a microwave irradiation method. The AgNPs synthesized by chemical-based microwave irradiation (Ag-C) were also used as a control material. The novel method produced a smaller size of nanostructures with good dispersion ability and less agglomeration than those from chemical synthesis. The antibacterial properties of AgNPs on Gram-negative bacteria Escherichia coli (E. coli) and Gram-positive bacteria Staphylococcus aureus (S. aureus) were investigated. The results revealed that AgNPs from both green synthesis and chemical-based methods inactivated both types of bacteria. The green-synthesized AgNPs from radish juice provided a higher percentage of inhibition of E. coli than that of S. aureus. The inactivation rates of the AgNPs increased with increasing concentration of AgNPs. As the concentration of the Ag/AgCl-R and Ag-R increased from 150 μg/mL to 300 μg/mL, complete inactivation required a reduced time for the reaction from 300 minutes to only 30 minutes. Finally, the Ag/AgCl-R and Ag-R offered high antibacterial activity while the Ag-T provided the lowest antibacterial activity. This work provides an alternative method for the eco-synthesis of antibacterial nanomaterials for water treatment.

scholarly journals Basics of the Development of Microwave Intensification of Upstream on the Example of Escherichia coli

2021 ◽  
Vol 10 (4) ◽  
pp. 72-80
Author(s):  
D. B. Kuznetsov ◽  
A. Yu. Mironov ◽  
V. A. Neschislyaev ◽  
I. L. Volkhin ◽  
A. M. Korolyuk ◽  
...  
Keyword(s):  
Microwave Irradiation ◽  
Microwave Radiation ◽  
Coli Strain ◽  
E Coli ◽  
Optimal Processing ◽  
Lux Operon ◽  
Adsorption Of Water ◽  
New Ideas ◽  
Culture Growth ◽  
Number Of Publications

Introduction. E. coli strains are the main microorganisms used for the production of a number of important biopharmaceutical products. There are no natural sources of microwave radiation on Earth, as it is absorbed by the upper atmosphere. No one doubts the importance of studying the biological effect of microwave radiation. The number of publications devoted to this problem is growing every year, and new ideas for the use of microwaves in drug production technology are emerging.Aim. Reveal the main effects of microwave irradiation and develop a technology for microwave intensification of E. coli culture growth.Materials and methods. This study presents the results of atomic force microscopy, refractometry, NMR relaxometry, turbidimetry, and lumimetry, demonstrating the possibility of microwave intensification of the cultivation process.Results and discussion. It was found that microwave irradiation leads to changes in the mobility of protons and the adsorption of water molecules on biopolymers and cells. These are the main links in the mechanism of "non-thermal" microwave action. A single microwave irradiation, depending on a number of parameters, can decrease or increase the growth of biomass. Studies of the bioluminescence of the E. coli strain with the lux-operon have shown that the optimal processing conditions do not negatively affect the luciferase production and metabolic activity of cells. Conclusion. The intensification procedure using microwave radiation can be considered a promising method and can provide new ideas for various applications in biotechnology.

scholarly journals Microwave-assisted multi-component synthesis of 3’-indolyl substituted pyrano[2,3-c]pyrazoles and their antimicrobial activity

2012 ◽  
Vol 77 (8) ◽  
pp. 983-991 ◽  
Author(s):  
Harshad Kathrotiya ◽  
Ranjan Patel ◽  
Manish Patel
Keyword(s):  
Antimicrobial Activity ◽  
Reaction Time ◽  
Microwave Irradiation ◽  
High Yield ◽  
Pyrazole Derivatives ◽  
Human Pathogens ◽  
Broth Microdilution ◽  
E Coli ◽  
Microwave Assisted ◽  
Derivatives Of

A series of pyrano[2,3-c]pyrazole derivatives of indole has been synthesized by multi-component reaction using conventional and microwave irradiation approach. Particularly valuable features of this method include high yield, broad substrate scope, shorter reaction time and straightforward procedure. Antimicrobial screening against eight human pathogens, namely B. subtilis, C. tetani, S. pneumoniae, S. typhi, V. cholerae, E. coli, A. fumigatus and C. albicans by employing broth microdilution MIC method as recommended by NCCLS.

scholarly journals Molecular Diversity via Tetrasubstituted Alkenes Containing a Barbiturate Motif: Synthesis and Biological Activity

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5868
Author(s):  
Ahmed Al-Sheikh ◽  
Masuma Begum ◽  
Bian Zhang ◽  
Richard A. Lewis ◽  
Nicholas E. E. Allenby ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Biological Activity ◽  
Candida Albicans ◽  
Microwave Irradiation ◽  
Ring Opening ◽  
Molecular Diversity ◽  
Pathogenic Fungus ◽  
Pyran Ring ◽  
E Coli

The synthesis of a molecularly diverse library of tetrasubstituted alkenes containing a barbiturate motif is described. Base-induced condensation of N1-substituted pyrimidine-2,4,6(1H,3H,5H)-triones with 5-(bis(methylthio)methylene)-2,2-dimethyl-1,3-dioxane-4,6-dione gave 3-substituted 5-(methylthio)-2H-pyrano[2,3-d]pyrimidine-2,4,7(1H,3H)-triones (‘pyranopyrimidinones’), regioselectively. A sequence of reactions involving ring-opening of the pyran moiety, displacement of the methylthio group with an amine, re-formation of the pyran ring, and after its final cleavage with an amine, gave tetrasubstituted alkenes (3-amino-3-(2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene)propanamides) with a diversity of substituents. Cleavage of the pyranopyrimidinones with an aniline was facilitated in 2,2,2-trifluoroethanol under microwave irradiation. Compounds were tested against Escherichia coli, Staphylococcus aureus, the yeast Schizosaccharomyces pombe, and the pathogenic fungus Candida albicans. No compounds exhibited activity against E. coli, whilst one compound was weakly active against S. aureus. Three compounds were strongly active against S. pombe, but none was active against C. albicans.

scholarly journals Synthesis and Microbial Activity of Novel 3-Methyl-2-pyrazolin-5-one Derivatives

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Mohamed S. Mostafa ◽  
Nasser M. Abd El-Salam ◽  
Othman Y. Alothman
Keyword(s):  
Microwave Irradiation ◽  
Microbial Activity ◽  
Butyric Acid ◽  
Reaction Rate ◽  
Considerable Increase ◽  
Ethyl Esters ◽  
E Coli

2-Oxo-2H-chromene-3-carbohydrazide derivatives2a,breact with 2-{[4-(substituted thiazol-2-yl)iminoethyl)-phenyl]hydrazono}-3-oxo-butyric acid ethyl esters4a–cto give 3-methyl-1-[(2-oxo-2H-chromen-3-yl) carbonyl]-4-{[4-(substituted thiazol-2-yl)iminoethyl)-phenyl]hydrazono}]-2-pyrazolin-5-one derivatives5a–f. A considerable increase in the reaction rate had been observed with better yield using microwave irradiation for the synthesis of compounds2a,b,3a–c, and5a–f. The synthesized products were tested againstB. subtilis,S. aureus, andE. colias well asC. albicanscompared with tetracycline and nystatin as reference drugs.

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