Preparation of a novel sandwich-type electrochemical immunosensor for AFP detection based on an ATRP and click chemistry technique

2020 ◽  
Vol 11 (4) ◽  
pp. 900-908 ◽  
Author(s):  
Xiaoqing Zhao ◽  
Na Wang ◽  
Hou Chen ◽  
Liangjiu Bai ◽  
Hui Xu ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Polymer Brush ◽  
Electrochemical Immunosensor ◽  
Functional Polymer ◽  
Sandwich Type ◽  
Synthesis Methodology ◽  
Application Scope

It is extremely important to explore the synthesis methodology and application scope of functional polymer brush-based nanocomposites.

An efficient method for the synthesis of a polymer brush via click chemistry and its ultrasensitive electrochemical detection of AFP

2018 ◽  
Vol 10 (20) ◽  
pp. 2390-2397 ◽  
Author(s):  
Dongcheng Yang ◽  
Jing Wang ◽  
Hou Chen ◽  
Hui Xu ◽  
Wenxiang Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Click Chemistry ◽  
Tumor Markers ◽  
Electrochemical Detection ◽  
Efficient Method ◽  
Polymer Brush ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection

A sensitive electrochemical immunosensor based on polyacrylonitrile-g-poly(hydroxyethylmethacrylate) (PAN-g-PHEMA)/graphene oxide (GO) composites was prepared for the ultrasensitive detection of tumor markers.

scholarly journals A New Approach for the Preparation of Functional Poly(acrylonitrile-g-glycidyl methacrylate) Polymer Brush

2017 ◽  
Author(s):  
Yating Wang ◽  
Wenxiang Wang ◽  
Hou Chen ◽  
Liangjiu Bai ◽  
Hui Xu
Keyword(s):  
Click Chemistry ◽  
Polymer Brushes ◽  
Glycidyl Methacrylate ◽  
Polymer Brush ◽  
Reactant Ratio ◽  
New Approach ◽  
Functional Polymer ◽  
Design And Synthesis ◽  
Accurate Design ◽  
Poly Acrylonitrile

A new approach for the preparation of polymer brush was developed via the union of controlled/“living” radical polymerization and click chemistry. The application of the approach realized the more quickly and accurate design and synthesis of the polymer brushes. A novel functional polymer brush of poly(acrylonitrile-g-glycidyl methacrylate) with tremendous application potential in the field of biology has been successfully synthesized via the new approach. The reaction conditions of click chemistry were optimized with the reaction time and the reactant ratio and judged from UV-vis spectra. Under the optimum of click chemistry, the novel functional polymer brushes were prepared. The GPC, FTIR analyses, 1H NMR spectrum and TGA were employed to ensure the successful synthesis of poly(acrylonitrile-g-glycidyl methacrylate) polymer brushes.

Electrochemical Immunosensors Based on Nanostructured Materials for Sensing of Prostate-Specific Antigen: A Review

2020 ◽  
Vol 28 ◽  
Author(s):  
Hayati Filik ◽  
Asiye Aslıhan Avan ◽  
Mustafa Özyürek
Keyword(s):  
Prostate Specific Antigen ◽  
Nanostructured Materials ◽  
Specific Antigen ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Electrochemical Immunosensors ◽  
Metal Metal ◽  
Different Types ◽  
Sandwich Type ◽  
Carbon Based Nanomaterials

: The prostate-specific antigen (PSA) has been considered a crucial serological marker for distinguishing prostate based cancer. This surveys recent progress in the construction of nanomaterial-based electrochemical immunosensors for a PSA. This review (from 2015 to 2020) reports the latest progress in PSA sensing based on the employ of different types of nanostructured materials. The most popular used nanostructured materials are metal, metal oxide, carbon-based nanomaterials, and their hybrid architectures utilized for distinct amplification protocols. In this review, the electrochemical immunosensors for prostate-specific antigen sensing are classified into three categories such as sandwich type@labeled, label free@nonlabeled and aptamer-based electrochemical immunosensor.

scholarly journals Sandwich Electrochemical Immunosensor for Early Detection of Tuberculosis Based on Graphene/Polyaniline-Modified Screen-Printed Gold Electrode

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3926 ◽  
Author(s):  
Umi Mohd Azmi ◽  
Nor Yusof ◽  
Norzila Kusnin ◽  
Jaafar Abdullah ◽  
Siti Suraiya ◽  
...  
Keyword(s):  
Gold Electrode ◽  
Differential Pulse ◽  
Sample Volume ◽  
Electrochemical Immunosensor ◽  
X Ray ◽  
Sandwich Type ◽  
Pulse Voltammetry ◽  
Culture Filtrate Protein ◽  
Cast Technique ◽  
Screen Printed

A rapid and sensitive sandwich electrochemical immunosensor was developed based on the fabrication of the graphene/polyaniline (GP/PANI) nanocomposite onto screen-printed gold electrode (SPGE) for detection of tuberculosis biomarker 10-kDa culture filtrate protein (CFP10). The prepared GP/PANI nanocomposite was characterized using Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM). The chemical bonding and morphology of GP/PANI-modified SPGE were studied by Raman spectroscopy and FESEM coupled with energy dispersive X-ray spectroscopy, respectively. From both studies, it clearly showed that GP/PANI was successfully coated onto SPGE through drop cast technique. Cyclic voltammetry was used to study the electrochemical properties of the modified electrode. The effective surface area for GP/PANI-modified SPGE was enhanced about five times compared with bare SPGE. Differential pulse voltammetry was used to detect the CFP10 antigen. The GP/PANI-modified SPGE that was fortified with sandwich type immunosensor exhibited a wide linear range (20–100 ng/mL) with a low detection limit of 15 ng/mL. This proposed electrochemical immunosensor is sensitive, low sample volume, rapid and disposable, which is suitable for tuberculosis detection in real samples.

A Novel Electrochemical Immunosensor Based on DNA/Fe3O4 (Core) /ZrO2（Shell）one Dimension Magnetic Probes for Carcino-Embryonic Antigen

2011 ◽  
Vol 80-81 ◽  
pp. 200-206 ◽  
Author(s):  
Fu Tao Hu ◽  
Yuan Zao Wu ◽  
Yu Ting Cao ◽  
Ning Gan
Keyword(s):  
Electrochemical Immunosensor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Good Precision ◽  
Current Signal ◽  
Separation And Purification ◽  
Rapid Separation ◽  
Catalytic Current ◽  
Nano Gold ◽  
Modified Dna ◽  
Sandwich Type

A novel and sensitive electrochemical enzyme-linked immunosorbent assay (ELISA) system of Hydroquinones (HQ)- peroxide hydantoin (CP) -horseradish-peroxidase (HRP) by using magnetic DNA linked core-shell Fe3O4@ZrO2（ZMPs）nanoparticles as labels and signal amplification was established. In the presence of carcinoembryonic antigen (CEA) analyte, the sandwich-type immunocomplex could be formed between HRP labeled CEA second antibody（CEA Ab2） modified DNA-ZMPs probes (DNA / (ZMPs-HRP-CEA Ab2) n) and nano-gold CEA modified glassy carbon electrode (GCE | CEA Ab1) as an immune electrode.The concentration of CEA was determined based on the current signal, which was generated in the reaction between HQ and CP catalyzed by HRP on the sandwich-type immunocomplex. ZMPs nanoparticles as label material can not only perform the rapid separation and purification of signal antibody on magnetic field, but also immensely enhance the labeled capacity of HRP-anti-CEA which can amplify the catalytic current signal. Furthermore, the electrode modification process was not required. The method provided a linear response range between 0.01 and 200 ng·mL-1with a detection limit of 4 pg·mL-1.Moreover, the proposed electrochemical ELISA method exhibited good precision, high sensitivity and acceptable reproducibility, and could be further developed for clinical detections of CEA and other biomarkers.

Electrolyte-Philic Electrode Material with a Functional Polymer Brush

2019 ◽  
Vol 11 (17) ◽  
pp. 16087-16095 ◽  
Author(s):  
Zhen Wang ◽  
Wenlin Zhang ◽  
Yongtao Tan ◽  
Ying Liu ◽  
Lingbin Kong ◽  
...  
Keyword(s):  
Electrode Material ◽  
Polymer Brush ◽  
Functional Polymer
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